Then they were cryofixed and freeze-substituted with Leica EM AFS2 in acetone containing 0.5 % glutaraldehyde and 0.2 % uranyl acetate and low-temperature embedded in Lowicryl HM20.
E.G. van Donselaar, B.M. Humbel
Electron Microscopy & Structure Analysis, Department of Molecular Cell Biology, Utrecht University, The Netherlands
Department of Cell Biology, University Medical Center Utrecht, The Netherlands