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Módulo DMi8 S Infinity TIRF

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    Imagen de célula viva

    Con un cambio de perspectiva desde componentes individuales del microscopio a una solución de captura y procesamiento de imágenes de células vivas totalmente operativa, Leica Microsystems integra…
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  • Fotomanipulación Leica

    Fotomanipulación

    El término fotomanipulación engloba una serie de técnicas que utilizan las propiedades de las moléculas fluorescentes para iniciar eventos y observar el comportamiento de compuestos dinámicos en…
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  • Microscopios de súper-resolución y nanoscopios

    Leica Microsystems es una empresa pionera en microscopía de superresolución, que trabaja en estrecha colaboración con científicos de primera línea en este campo. Leica Microsystems es el único…
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  • [Translate to spanish:] Leica Cell Biology

    Biología celular

    Descubra cómo puede extraer el máximo rendimiento a sus investigaciones en materia de biología celular y amplíe sus descubrimientos con las soluciones de generación de imágenes de Leica Microsystems.
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  • Virología

    ¿Su interés en investigación se centra en infecciones víricas y enfermedades? Descubra cómo puede adquirir conocimientos sobre virología con soluciones de generación de imágenes y preparación de…
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  • Images from 2 time-lapse series of the same Sf21 moth larval cells
    Science Lab Topic: Widefield Microscopy

    Studying Actin Cytoskeleton and Microfilament Assembly

    This article demonstrates how actin microfilament assemblies can be clearly visualized with superior resolution at the plasma membrane of a cell using a DMi8 S Infinity TIRF High Power imaging…
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  • Science Lab Topic: Fluorescence Microscopy

    Plant Cell Development and Morphogenesis

    The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin…
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  • Science Lab Topic: TIRF Microscopy

    TIRF Publication List

    This monthly updated references list presents current papers using Leica AM TIRF in the major application fields for TIRF microscopy.
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  • Science Lab Topic: Live-Cell Imaging

    Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex

    Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the…
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  • Science Lab Topic: Super-Resolution

    Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex

    Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been extensively investigated, the…
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  • Science Lab Topic: Super-Resolution

    Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles

    Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic vesicles have identical protein content has been…
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  • Science Lab Topic: TIRF Microscopy

    Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy

    Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique…
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  • Science Lab Topic: TIRF Microscopy

    Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

    The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
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  • Widefield fluorescence image of brest carcinoma tumor cells expressing GFP tagged cell adhesion Molecule CD44 that is expressed on the cell membrane, imagined in TIRF.
    Science Lab Topic: TIRF Microscopy

    Total Internal Reflection Fluorescence (TIRF) Microscopy

    Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
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  • Science Lab Topic: TIRF Microscopy

    TIRF Microscopy Explores Cellular Transport Processes

    Due to their special role in organ function and the exchange of biological components some body cells developed certain polarization characteristics. These are reflected in differences of their plasma…
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  • Science Lab Topic: Live-Cell Imaging

    TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells

    Application of TIRF microscopy (Total Internal Reflection Fluorescence) allows the visualization of structures at the apical surface of polarized epithelial cells that have been hidden in conventional…
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  • Science Lab Topic: Live-Cell Imaging

    Exploring Cell Logistics

    Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a…
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