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Leica EM VCT500 Sample Transfer Leica Leica Microsystems

Sistema de criotransferencia en vacío Leica EM VCT500

  • High Pressure Freezing with Light Stimulation

    High Pressure Freezing with Light Stimulation

    Sun screen lotion was carefully filled in the 100 μm incision of a 3 mm copper/gold plated flat carrier and covered with 3 mm sapphire disk. The sun screen lotion sample was then high pressure frozen with a Leica EM ICE with and subsequently without light stimulation. The light stimulated samples were exposed to a UV light for 500 milliseconds.
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  • Brief Introduction to Freeze Fracture and Etching

    Brief Introduction to Freeze Fracture and Etching

    Freeze fracture describes the technique of breaking a frozen specimen to reveal internal structures. Freeze etching is the sublimation of surface ice under vacuum to reveal details of the fractured face that were originally hidden. A metal/carbon mix enables the sample to be imaged in a SEM (block-face) or TEM (replica). It is used to investigate for instance cell organelles, membranes, layers and emulsions.
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  • Brief Introduction to Coating Technology for Electron Microscopy

    Brief Introduction to Coating Technology for Electron Microscopy

    Coating of samples is required in the field of electron microscopy to enable or improve the imaging of samples. Creating a conductive layer of metal on the sample inhibits charging, reduces thermal damage and improves the secondary electron signal required for topographic examination in the SEM.
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