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Images à caractérisation spectrale Leica TCS SP8 X

  • primary murine pre B cells expressing the eCALWY-6 Zn2+

    Researchers use Functional Imaging to Elucidate the Role of Cellular Zn²⁺ in Human Immunity

    An international consortium of researchers has found a new mutation in a gene harboring a reticulum-to-cytoplasm zinc transporter. The gene is called ZIP7 and it is responsible for the early onset of…
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  • A Molecular Link between Cell Migration and Vascular Disease

    Blood vessels transport vital nutrients and oxygen to all the cells in the body. Guided by a complex signaling network, endothelial cells sprout, proliferate, and migrate to form those vessels. One of…
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  • You Shall Not Pass! Time-gated Detection Takes Care of Endogenous Fluorescence in Plant Research.

    Yutaka Kodama is Associate Professor at the Biosciences Education Research Center, Utsunomiya University, Japan. He graduated from Saga University, Faculty of Agriculture, in 2004 and received his PhD…
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  • Chemical Basis for Alteration of an Intraocular Lens Using a Femtosecond Laser

    The chemical basis for the alteration of the refractive properties of an intraocular lens with a femtosecond laser was investigated. Three different microscope setups have been used for the study:…
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  • Autocrine Regulation of Stomatal Differentiation Potential by EPF1 and ERECTA-LIKE1 Ligand-receptor Signaling

    Development of stomata, valves on the plant epidermis for optimal gas exchange and water control, is fine-tuned by multiple signaling peptides with unique, overlapping, or antagonistic activities.…
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  • The Apical Ectodermal Ridge of the Mouse Model of Ectrodactyly Dlx5;Dlx6−/− Shows Altered Stratification and Cell Polarity, which are Restored by Exogenous Wnt5a Ligand

    The congenital malformation split hand/foot (SHFM) is characterized by missing central fingers and dysmorphology or fusion of the remaining ones. During limb development, the apical ectodermal ridge…
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  • Contributions of Microbiome and Mechanical Deformation to Intestinal Bacterial Overgrowth and Inflammation in a Human Gut-on-a-Chip

    A human gut-on-a-chip microdevice was used to coculture multiple commensal microbes in contact with living human intestinal epithelial cells for more than a week in vitro and to analyze how gut…
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  • The Oncogenic Triangle of HMGA2, LIN28B and IGF2BP1 Antagonizes Tumor-Suppressive Actions of the let-7 Family

    The tumor-suppressive let-7 microRNA family targets various oncogene-encoding mRNAs. We identify the let-7 targets HMGA2, LIN28B and IGF2BP1 to form a let-7 antagonizing self-promoting oncogenic…
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  • ERK2 and Akt are Negative Regulators of Insulin and Tumor Necrosis Factor-α Stimulated VCAM-1 Expression in Rat Aorta Endothelial Cells

    Diabetes is quickly becoming the most widespread disorder in the Western world. Among the most prevalent effects of diabetes is atherosclerosis, which in turn is driven in part by inflammation. Both…
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  • Video: The White Light Laser – How to Effectively Excite Multiple Fluorophores with a Single Light Source

    The Leica White Light Laser produces a continuous spectral output between the wavelengths of 470 and 670 nm. It allows you to select 8 excitation lines from 3 trillion unique combinations for…
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  • How to Choose the Right Confocal Microscope for Your Lab?

    Confocal microscopy has come a very long way since its invention more than a half-century ago. Today, with novel technology driven by leading imaging companies, it has become the standard for…
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  • Video Talk by Karl Deisseroth: Optogenetics

    Optogenetics is a combination of genetics and optics to achieve a gain or loss of function of biochemical events such as action potentials in a particular neuron or tissue. Opsin genes encode proteins…
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  • Clearing Procedures for Deep Tissue Imaging

    Multi-channel multiphoton microscopy with dedicated optics for CLARITY. Why clearing? Curiosity is human nature. And nothing attracts as much curiosity as the inside of living organisms. While in…
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  • Map the Brain with CLARITY

    Imaging whole brains with CLARITY and multiphoton microscopy. Image a whole brain without sectioning? Investigate neuronal circuits without reconstruction? Perform molecular phenotyping without…
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  • Acousto Optics in True Confocal Spectral Microscope Systems

    Acousto-optical elements have successfully replaced planar filters in many positions. The white confocal, regarded as the fully spectrally tunable confocal microscope, was not possible without this…
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  • Spectral Detection – How to Define the Spectral Bands that Collect Probe-specific Emission

    To specifically collect emission from multiple probes, the light is first separated spatially and then passes through a device that defines a spectral band. Classically, this is a common glass-based…
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  • Multiple Microscopy Modes in a Single Sweep with Supercontinuum White Light

    Lasers have been critical to the advancement on confocal microscopy, and the white light laser (WLL) offers particular advantages. Finessing WLL output for bioimaging is a complex task, though, and…
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  • The White Confocal – Spectral Gaps Closed

    This article summarizes the development and differences in design and functionality of confocal technology as far as spectral properties are concerned, from classical filter-based excitation and…
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  • The White Confocal

    Modern biomedical research is currently dominated by imaging and measuring with optical microscopes. One branch of the microscopy technology is confocal microscopy. For correlation purposes,…
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  • Optogenetics

    Optogenetics is a technique that allows light-controlled responses of transfected cells. The cells are genetically modified by introduction of genes that code for light-induced channels or ion pumps.…
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  • Sensors for True Confocal Scanning

    In this article, advantages and disadvantages of different types of sensors for single point true confocal scanning devices are discussed. Traditionally, photomultiplier tubes have been employed in…
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  • White Light Laser

    The perfect light source for confocal microscopes in biomedical applications has sufficient intensity, tunable color and is pulsed for use in lifetime fluorescence. Furthermore, it should offer means…
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  • Quantitative Fluorescence

    Seeing is believing – and measuring is knowing. Microscopes generate images that are not only used for illustration, but are also subject to quantification. More advanced techniques use illumination…
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  • How to Study Protein Recruitment to DNA Lesions by a Combination of UV Laser and White Light Laser

    Understanding how DNA lesions are optimally repaired is of functional significance, especially from the view of genome karyotype stability.
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  • Beam Splitting

    Fluorescence Microscopy usually employs incident light illumination. This requires a device that directs the light for illumination into the sample and transmits the light emitted by the sample to the…
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  • Spectral Imaging

    To separate fractions of the emission for recording channels that reproduce the emission of individual fluorochromes, it is necessary to spatially disperse the emission spectrally. This is possible by…
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  • Confocal Excitation

    Fluorescence excitation needs specifically colored light. In confocal microscopy, multiline lasers or laser batteries are classically used. This requires devices that pick the requested lines fitting…
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  • Förster Resonance Energy Transfer (FRET)

    The Förster Resonance Energy Transfer (FRET) phenomenon offers techniques that allow studies of interactions in dimensions below the optical resolution limit. FRET describes the transfer of the energy…
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  • Brighter Fluorescence by Resonant Scanning

    Fast True Confocal Scanning reduces photobleaching and increases the fluorescence yield at identical acquisition times. The long-lasting triplet state (or any other “dark state”) is less populated…
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  • Pinhole Controls Optical Slicing

    True confocal scanning microscopes (TCS) use a variable detection pinhole. Good optical sectioning tries to use just the inner core of the PSF.
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  • Confocal Optical Section Thickness

    Confocal microscopes are employed to optically slice comparably thick samples.
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  • Lasers for Confocal

    True confocal scanning microscopy (TCS) requires bright diffraction-limited illumination.
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  • Confocal Microscopy

    “Confocal Microscopy” refers to a particular optical microscope that allows recording optical sections. Optical sectioning is achieved in a confocal system by illuminating and observing a single…
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  • Choose Your Excitation Wavelength

    Although time correlated single photon counting (TCSPC) is the method of choice for fluorescence lifetime quantification, it requires dedicated instrumentation including a pulsed laser source, a…
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