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Alberto Diaspro, Prof.


Alberto Diaspro is Director of the Department of Nanophysics at the Italian Institute of Technology (IIT) as well as Professor of Applied Physics at the Department of Physics, University of Genoa. He also is the scientific coordinator of the Ph.D. Course on Nanosciences at the University of Genoa within an IIT program.

Alberto Diaspro is President of EBSA (European Biophysical Societies' Association), of OWLS (Optics with Life Sciences) and founder of the Nanoscale Biophysics Subgroup of the Biophysical Society. He has directed the design and realization of the first Italian Nanoscopy architecture at the Italian Institute of Technology.

Alberto Diaspro has more than 200 international publications, H index=26 and is editor of three international key books in the Microscopy area: “Nanoscopy and Multidimensional Optical Fluorescence Microscopy”  (2010) Taylor &Francis Group A Chapman & Hall Book Crc Press, pp.448; “Optical Fluorescence Microscopy: from the spectral to the nano dimension.” (2010) Springer-Verlag Berlin and Heidelberg GmbH & Co. K , pp.256.;  “Confocal and Two-Photon Microscopy: Foundations, Applications and Advances” (2001) Wiley-Liss, pp.580.

Alberto Diaspro founded LAMBS in 2003 (Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy). His experience is related with the design, realization and utilization of optical and biophysical instrumentation as conventional and confocal microscopy, two-photon fluorescence microscopy and spectroscopy architecture, differential scanning calorimetry, scanning probe microscopy (STM, SNOM, AFM), polarized light scattering, signal and image digital processing. At his lab, the first Italian two-photon excitation architecture for microscopy and spectroscopy with extension to single molecule imaging was realized. Also, a nanotechnological approach for the realization of nanobiorobot, hybrid nanostructured devices made by living cells and polylelctrolyte layers, has been recently developed within the nanocomposite materials framework.

  • Alberto Diaspro Comments on Receiving the Emily M. Gray Award 2014

    Alberto Diaspro, Director of the Department of Nanophysics at the Italian Institute of Technology (IIT), has received the Emily M. Gray Award 2014 for dedicating his career to mentoring students and serving as the major force in organizing international biophysics workshops that showcase the talent of young researchers. The Biophysical Society ( yearly rewards scientists who particularly stood out for their contributions to education in biophysics. In this brief video he comments on what it was like to receive the Award and on his contributions to education and mentorship in biophysics.
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  • Abstracts of the 3rd European Super-Resolution User-Club Meeting

    The 3rd meeting of the Leica Super-Resolution User Club was held from June 17th to 19th, 2013 in collaboration with Alberto Diaspro and the Italian Institute of Technology (IIT) in Genoa. Confocal and widefield super-resolution users from ten European countries took three days’ out to deepen their knowledge on super-resolution techniques and applications and make use of an opportunity for full exchange of experiences.
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  • Abstracts of the 2nd European Super-Resolution User-Club Meeting

    The 2nd meeting of the Leica Super-resolution User club was held from September 25 to 27, 2012 in collaboration with the Science for Life Laboratory at the Karolinska Institute, Stockholm, Sweden. With a mixture of engaging talks by key experts in the field of super-resolution microscopy and stimulating discussion sessions, the meeting proved as popular as last year’s event, attracting a wide range of scientists interested in both confocal and widefield super-resolution and sample preparation techniques.
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  • Developments in Multiphoton Excitation Microscopy

    Basics, history, and applications of multiphoton microscopy. Honouring Goeppert-Mayer’s prediction of simultaneous two-photon absorption by an atom or molecule reported in the 1930s in her PhD dissertation thesis, we can state that multiphoton excitation (MPE) microscopy, more frequently identified with two-photon excitation (2PE) fluorescence microscopy, is a key microscopy method in many areas from medicine to biology, from biophysics to materials science.
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  • Order versus Disorder

    In modern biomaterial design the generation of an environment mimicking some of the extracellular matrix features is envisaged to support molecular cross-talk between cells and scaffolds during tissue formation/remodeling. In bone substitutes chemical biomimesis has been particularly exploited; conversely, the relevance of pre-determined scaffold architecture for regenerated bone outputs is still unclear.
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  • Abstracts of the First European Super-Resolution User Club Meeting

    The first European Super-resolution User Club meeting took place from October 27 to 29 in Göttingen, Germany. Prof. Stefan Hell, the inventor of the STED technology, has hosted this first meeting. The user club is aimed at pioneering researchers from the European scientific community, who are early adopters and developers of super-resolution techniques.
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  • Webinar: The Solution for Deep Imaging

    Imaging of thick specimen using multiphoton microscopy. Multiphoton microscopy is the method of choice for non-invasive deep-penetration fluorescence microscopy of thick highly scattering samples. Good results have already been obtained with a diversity of specimen, e.g. lymphatic organs, kidney, heart, skin and brain (slices as well as whole organs, fixed specimen as well as living cells).
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  • Stem Cell Biology in Cancer Research

    The comprehension of stem cell biology and its molecular basis is now acquiring paramount importance in cancer research. The need to look at a single, possibly living, cell makes fluorescence microscopy and confocal microscopy invaluable allies in the study of stem cells.
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  • The First Supercontinuum Confocal that Adapts to the Sample

    Until now, biological and medical research fluorescence imaging in multi-user facilities or institutes has been limited by the type or number of dyes that could be excited. The Leica TCS SP5 X supercontinuum confocal unites the broadband capabilities of the Leica TCS SP5 AOBS® and the freedom and flexibility to select any excitation line within the continuous range of 470 to 670 nm.
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  • From Molecules to Tissues

    Cancer research using confocal and multiphoton microscopy. Sequencing of the human genome stimulated a radical change in the approach to biomedical research. The comprehension of the mechanisms regulating life gained a scale-up in throughput to speed up the retrieval of data for a global vision of a system of incomparable complexity.
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  • FRET Measurements on Fuzzy Fluorescent Nanostructures

    In the last decade, fluorescence resonance energy transfer (FRET) has become a useful technique for studying intermolecular interactions applied to the analysis of biological systems. Although FRET measurements may be very helpful in the comprehension of different cellular processes, it can be difficult to obtain quantitative results, hence the necessity of studying FRET on controllable systems.
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  • 4D Photoactivation of pa-GFP in Living Cells Using Two-Photon Excitation Laser Scanning Microscopy

    We report about two-photon activation of a photoactivatable derivative of the Aequorea Victoria green fluorescent protein (pa-GFP). This special form of the molecule increases its fluorescence intensity when excited by 488 nm after irradiation with high intensity light at 413 nm. Two-photon photoactivation produces an effective real three-dimensional (3D) localization of the molecular switching of pa-GFP in the bright state.
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  • Multi-photon Excitation Microscopy

    Advanced microscopical techique for life science: multiphoton microscopy. Multi-photon excitation (MPE) microscopy plays a growing role among microscopical techniques utilized for studying biological matter. In conjunction with confocal microscopy it can be considered the imaging workhorse of life science laboratories. Its roots can be found in a fundamental work written by Maria Goeppert Mayer more than 70 years ago. Nowadays, 2PE and MPE microscopes are expected to increase their impact in areas such biotechnology, neurobiology, embryology, tissue engineering, materials science where imaging can be coupled to the possibility of using the microscopes in an active way, too.
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