Phase Contrast and Microscopy
This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.
How to Prepare your Specimen for Immunofluorescence Microscopy
Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM,…
Fluorescent Dyes
A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP –…
暗視野顕微鏡
暗視野コントラスト法は、生体試料の構造や物質試料の不均一な特徴から生じる光の回折や散乱を利用する方法です。
位相コントラスト
位相差顕微鏡では、染色することなく、多くの種類の生物試料の構造をより優れたコントラストで観察できます。
Introduction to Widefield Microscopy
This article gives an introduction to widefield microscopy, one of the most basic and commonly used microscopy techniques. It also shows the basic differences between widefield and confocal…
Photoactivatable, Photoconvertible, and Photoswitchable Fluorescent Proteins
Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…
Metallography with Color and Contrast
The examination of microstructure morphology plays a decisive role in materials science and failure analysis. There are many possibilities of visualizing the real structures of materials in the light…
Optical Contrast Methods
Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the…
