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Leica EM HPM100 Cryo Preparation Leica Leica Microsystems

High Pressure Freezer for Cryofixation of Biological & Industrial samples Leica EM HPM100

  • Dynamic changes in the subcellular distribution of the tobacco ROS-producing enzyme RBOHD in response to the oomycete elicitor cryptogein, Elodie Noirot1,*, Christophe Der2,*, Jeannine Lherminier1, Franck Robert3, Pavla Moricova3,4, Kiên Kiêu5, Nathalie Leborgne-Castel2, Françoise Simon-Plas3 and Karim Bouhidel2,†; 1 INRA, UMR1347 Agroécologie, ERL CNRS 6300, Plateforme DImaCell, Centre de Microscopie INRA/Université de Bourgogne, BP, 86510, F-21065 Dijon Cedex, France; 2 Université de Bourgogne, UMR1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21065 Dijon Cedex, France; 3 INRA, UMR1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21065 Dijon Cedex, France; 4 Present address: Department of Biochemistry, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, CZ-783 71 Olomouc, Czech Republic; 5 INRA, UR341 Mathématiques et Informatique Appliquées, F-78352 Jouy-en-Josas Cedex, France; * These authors contributed equally to this work.; To whom correspondence should be addressed.; Journal of Experimental Botany, doi:10.1093/jxb/eru265; http://jxb.oxfordjournals.org/content/early/2014/06/30/jxb.eru265.short



  • HIGHLY METHYL ESTERIFIED SEEDS is a Pectin Methyl Esterase involved in embryo development, Gabriel Levesque-Tremblaya, Kerstin Müllerb, Shawn D. Mansfieldc, George W. Haughna,1; a Department of Botany, c Faculty of Forestry, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada; b Department of Biological Science, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada; c Department of Wood Science, Faculty of Forestry, University of British Columbia, Vancouver, BC V6T 1Z4, Canada; American Society of Plant Biologists; DOI:10.1104/pp.114.255604; http://www.plantphysiol.org/content/early/2015/01/08/pp.114.255604.short
  • Formation and excretion of autophagic plastids (plastolysomes) in Brassica napus embryogenic microspores, VerónicaParra-Vega,PatriciaCorral-Martínez,AlbaRivas-SendraandJoseM.Seguí-Simarro*;  Cell Biology Group, COMAV Institute, Universitat Politècnica de València, Valencia, Spain¸*Correspondence: Jose M. Seguí-Simarro, COMAV- Universitat Politècnica de València, Ciudad Politécnica de la Innovación, Edificio 8E-EscaleraI,Caminode Vera,s/n,46022Valencia,Spain; ORIGINAL RESEARCH ARTICLE published: 19February2015 doi: 10.3389/fpls.2015.00094; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333807/
  • Herpes Simplex Virus 1 Us3 Deletion Mutant is Infective Despite Impaired Capsid Translocation to the Cytoplasm, Peter Wild 1,2,*, Sabine Leisinger 1, Anna Paula de Oliveira 2, Elisabeth M. Schraner 1,2, Andres Kaech 3, Mathias Ackermann 2 and Kurt Tobler 2; 1 Institute of Veterinar Anatomy, Winterthurerstrasse 260, CH-8057 Zürich, Switzerland; 2 Institute of Virology, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland; 3 Center for Microscopy and Image Analysis, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland; * Author to whom correspondence should be addressed;  Viruses 2015, 7, 52-71; doi:10.3390/v7010052; http://www.mdpi.com/1999-4915/7/1/52/htm
  • New views of tapetum ultrastructure and pollen exine development in Arabidopsis thaliana, Teagen D. Quilichini, Carl J. Douglas and A. Lacey Samuels*Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, BC V6 T 1Z4, Canada; Oxford University Press on behalf of the Annals of Botany Company, doi:10.1093/aob/mcu042, http://aob.oxfordjournals.org/content/early/2014/04/08/aob.mcu042.short


  • Extended Ultrastructural Characterization of Chordoma Cells: The Link to New Therapeutic Options, Dagmar Kolb1,2, Elisabeth Pritz2, Bibiane Steinecker-Frohnwieser3, Birgit Lohberger4, Alexander Deutsch5, Thomas Kroneis2, Amin El-Heliebi2, Gottfried Dohr2, Katharina Meditz1, Karin Wagner1, Harald Koefeler1, Gerd Leitinger2, Andreas Leithner4, Bernadette Liegl-Atzwanger6Dagmar Zweytick7, Beate Rinner1*; 1 Center for Medical Research, Medical University of Graz, Graz, Austria, 2 Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Graz, Austria, 3 Ludwig Boltzmann Institute for Rehabilitation of Internal Diseases, Ludwig Boltzmann Cluster for Rheumatology, Balneology and Rehabilitation, Saalfelden, Austria, 4 Department of Orthopaedic Surgery, Medical University of Graz, Graz, Austria, 5 Division of Hematology, Department of Internal Medicine, Medical University of Graz, Graz, Austria, 6 Institute of Pathology, Medical University of Graz, Graz, Austria, 7 Biophysics Division, Institute of Molecular Biosciences, University of Graz, Graz, Austria; PLOS ONE | DOI:10.1371/journal.pone.0114251; http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0114251#pone-0114251-g002
  • The transmembrane protein Macroglobulin complement-related is essential for septate junction formation and epithelial barrier function in Drosophila, Tilmann Bätz++, Dominique Förster*,++ and Stefan Luschnig§ Institute of Molecular Life Sciences and PhD Program in Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland, Development141, 899-908, received August 5, 2013, accepted December 8, 2013,
  • Ultrafast endocytosis at mouse hippocampal synapses, Watanabe S1, Rost BR2, Camacho-Pérez M3, Davis MW1, Söhl-Kielczynski B4, Rosenmund C4, Jorgensen EM1. 1Department of Biology and Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112, Utah, USA.21] Neuroscience Research Centre, Charité Universitätsmedizin, 10117 Berlin, Germany [2] German Center for Neurodegenerative Diseases (DZNE), 10117 Berlin, Germany. [3].31] Neuroscience Research Centre, Charité Universitätsmedizin, 10117 Berlin, Germany [2].4Neuroscience Research Centre, Charité Universitätsmedizin, 10117 Berlin, Germany, Nature, 2013 Dec 12, 504 (7479):242-7 doi: 10.1038 nature 12809, Epub 2013 Dec 4

  • The Presynaptic Dense Projection of the Caenorhabiditis elegans Cholinergic Neuromuscular Junction Localizes Synaptic Vesicles at the Active Zone through SYD-2/Liprin and UNC-10/RIM-Dependent Interactions, Christian Stigloher, Hong Zhan, Mei Zhen, Janet Richmond, and Jean-Louis Bessereau, The Journal of Neuroscience, 23 March 2011,  http://www.jneurosci.org/content/31/12/4388.short
  • Electron Tomography and immunogold Labelling as Tools to Analyse De Novo Assembly of Plant Cell Walls, Marisa S. Otegui, The Plant Cell Wall, Methods in Molecular Biology, 2011, Volume 715, 123-140, http://www.springerlink.com/content/rj3033344457u26k/#section=839467&page=1&locus=0
  • A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues, K.L.MCDONALD, Journal of Microscopy, Volume 235, Issue 3, pages 273-281, September 2009, http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2818.2009.03218.x/full
  • Preparation of cryofixed cells for improved 3D ultrastructure with scanning transmission electron tomography, Katharina Höhn, Michaela Sailer, Li Wang, Myriam Lorenz, Marion E. Schneider and Paul Walther, Histochemistry and Cell Biology, Volume 135, Number 1, 1-9, November 2010
  • Immunoelectron Microscopy of Cryofixed Freeze-Substituted Mammalian Tissue Culture Cells, Akira Sawaguchi, Immunoelectron Microscopy, Methods in Moledular Biology, Volume 657, Part 2, 181-190, 2010, http://www.springerlink.com/content/l0672m77t34440j5/#section=726826&page=1
  • Secondary Cell Wall Deposition in Developing Secondary Xylem of Poplar, Minako Kaeda, Kim Rensing, Lacey Samuels, Journal of Integrative Plan Biology, Volume 52, Issue 2, pages 234-243, February 2010,
  • Strategies for Imaging Microtubules in Plant Cells, Andreas Holzinger, Eiko Kawamura, and Geoffrey O. Wasteneys, Cytoskeleton Methods and Protocols, Methods in Molecular Biology, Volume 586, Part 3, 243 - 262, 2010,
  • Leishmania parasitophorous vacuoles interact continuously with the host cells's endoplasmic reticulum; parasitophorous vacuoles are hybrid compartments, Blaise Ndjamen, Byung-Ho Kang, Kiyotaka Hatsuzawa, Peter E. Kima, Cellular Microbiology, Volume 12, Issue 10, pages 1480-1494, October 2010, http://onlinelibrary.wiley.com/doi/10.1111/j.1462-5822.2010.01483.x/full
  • A wide diversity of previously undetected free-living relatives of diplomonads isolated from marine/saline habitats, Martin Kolisko, Jeffrey D. Silberman, Ivan Cepicka,, Naoji Yubuki, Kiyotaka Takishita, Akinori Yabuki, Brioan S. Leander, Isao Inouye, Yuji Inagaki, Andrew J. Roger, Alaistair G.B. Simpson, Enrivonmental Microbiology, Volume 12, Issue s10, pages s2700-2710, October 2010, http://onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2010.02239.x/full