Fast super-resolution for every task
HyVolution 2 helps you turn your Leica TCS SP8 confocal system into a super-resolution system without compromise. With the photon budget afforded by Leica HyD hybrid detectors, you have the freedom to use HyVolution 2 with all standard fluorescence staining protocols and fluorescent proteins.
Seamless integration with Huygens by SVI: you have the freedom to use fast parallel processing on GPU. HyVolution 2 thus becomes part of the image acquisition process making it the fastest super-resolution system in every respect.
Developed with our partners from SVI.
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Unlike super-resolution systems using a single external detection channel, HyVolution 2 integrates seamlessly with all confocal imaging modalities.
Therefore, you can record all five channels simultaneously – without the need for sequential scanning, which would only slow down the acquisition speed.
With HyVolution 2, you can study interactions of multiple molecules correctly at full speed.
Video: HyVolution in multicolor live cells. All three colors were acquired simultaneously (no sequential scanning) in 5D. HeLa Kyoto cells. GPI-YFP (yellow), H2B-mCherry (blue), Tubulin-SiR (red).
Multiple colors in up to five channels simultaneously
HyVolution 2 allows multicolor acquisition with high speed. The filter-free spectral detection system of the Leica TCS SP8 can collect up to five simultaneous channels.
This allows much faster parallel data collection compared to single channel solution: You get eight multicolor images with a Leica TCS SP8 with HyVolution 2 in the same time that single-detector solutions take to provide only one single-color image.
Super-resolution going live
Standard confocal systems sacrifice speed for resolution.
HyVolution 2 does not limit the speed of image acquisition and also provides the full framerate of the Leica TCS SP8 (40 fps @ 512 x 512 pixels) with multicolor super-resolution.
This allows you to capture events that occur within milliseconds and observe cellular dynamics in great detail and with unprecedented sharpness.