Enjoy spectral freedom with 4Tune
The number of transgenic markers evolves constantly and rapidly. The SP8 DIVE keeps apace with these developments and can adapt to existing and new transgenic markers with just a few mouse clicks.
Spectral freedom results from the new groundbreaking 4Tune technology for non-descanned detection. The 4Tune detector allows you to adapt to the full emission and to separate strongly overlapping spectra – without mathematical restoration.
The SP8 DIVE enables you to capture twice as much of the fluorescence signal, which translates into improved penetration depth, imaging speed, or less phototoxicity for in vivo imaging.
"The conventional dichroics are never optimal to distinguish all fluorophores, but with the spectral detectors this is now possible and much easier, since we can really optimize the wavelength for each fluorophore you want to detect."
Prof. Dr. Jacco van Rheenen. University of Utrecht, the Netherlands
DIVE with ease – the 4Tune detector
The 4Tune non-descanned detector can be equipped with 2 to 4 detection units and is freely configurable with hybrid detectors (HyDs) photomultipliers (PMTs), or a mix of both. The emission light is separated by a combination of variable dichroics and bandpass filters. The tuning ranges from 380 up to 800 nm wavelength.
The 4Tune user interface allows you to optimize the emission setting for multiple transgenic markers by simple drag and drop. Due to its clear and intuitive design, operating it is easy and requires minimal training.
With the SP8 DIVE, you are equipped for every existing and newly developed transgenic marker and future proved for new developments!
Explore new dimensions in depth
With the SP8 DIVE, you can tune for the deepest insight and finest detail. All excitation beams can be optimally adjusted independently for any objective using the new Vario Beam Expander (VBE).
The VBE allows for optimized colocalization and the right balance of resolution and depth in line with your research question.
Optimize for depth and resolution with the Vario Beam Expander
The Leica Vario Beam Expander VBE combines tunable beam diameter and tunable divergence. This offers you maximum depth, best resolution and full color correction.
Tunable beam diameter for best balance of resolution and depth
You can choose the best individual balance between resolution and energy input by tuning the diameter of the beam. When you widen the beam, you obtain homogeneous illumination of the back focal plane. If used in combination with a high numerical aperture, maximal resolution is reached.
Narrowing the beam will allow more energy input into the focal volume, resulting in a better penetration of the sample and deeper imaging. With the VBE, you can adjust the beams independently, for up to four different