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SP8 FALCON Confocal Microscopes Leica Leica Microsystems

Lifetime imaging in an instant SP8 FALCON

  • Live Cell Imaging

    Live Cell Imaging

    Shifting perspective from single microscope components to a full working live cell imaging solution, Leica Microsystems integrates microscope, LAS X imaging software, cameras, and dedicated…
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  • Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope
    Science Lab Topic: Quantitative Fluorescence

    Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope

    Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by…
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  • Free Webinar On-Demand: Super-resolved STED spectroscopy
    Science Lab Topic: Super-Resolution

    Free Webinar On-Demand: Super-resolved STED spectroscopy

    Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules.
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  • FLIM FRET and Biosensors: Versatile Tools for Biomedical Research
    Science Lab Topic: Quantitative Fluorescence

    FLIM FRET and Biosensors: Versatile Tools for Biomedical Research

    Fluorescence Lifetime Imaging (FLIM) in combination with Förster Resonance Energy Transfer (FRET) has proven to be very beneficial for investigations in biomedical research for a wide range of…
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  • Lifetime – a Proper Alternative
    Science Lab Topic: Quantitative Fluorescence

    Lifetime – a Proper Alternative

    „Way too complicated!“ - the notorious feedback when it comes to fluorescence lifetime measurements. This will change now! New technologies and new concepts for data evaluation, all implemented in…
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  • FCS - Fluorescence Correlation Spectroscopy
    Science Lab Topic: Quantitative Fluorescence

    FCS - Fluorescence Correlation Spectroscopy

    FCS is a fluorescence-based measurement method. Fluorescent molecules passing through a strongly focused, fixed laser beam are excited for fluorescence emission. After passing a confocal pinhole, the…
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  • FLIM FRET - Fluorescence Resonance Energy Transfer
    Science Lab Topic: Quantitative Fluorescence

    FLIM FRET - Fluorescence Resonance Energy Transfer

    A typical application of FLIM is FLIM-FRET. FRET is a well-established technique to study molecular interactions. It scrutinizes protein binding and estimates intermolecular distances on an Angström…
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  • FLCS - Fluorescence Lifetime Correlation Spectroscopy
    Science Lab Topic: Quantitative Fluorescence

    FLCS - Fluorescence Lifetime Correlation Spectroscopy

    Essentially, FCS can be performed with a continuous-wave laser. Using pulsed lasers allows even more sophisticated analysis possibilities, such as time-gated FCS or Fluorescence Lifetime Correlation…
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  • FLIM - Fluorescence Lifetime Imaging
    Science Lab Topic: Quantitative Fluorescence

    FLIM - Fluorescence Lifetime Imaging

    The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
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  • SP FLIM - Spectral Fluorescence Lifetime Imaging
    Science Lab Topic: Quantitative Fluorescence

    SP FLIM - Spectral Fluorescence Lifetime Imaging

    The SP8 FALCON is the ideal tool for spectral FLIM detection. No emission filters in front of the FLIM detectors are necessary. This grants a much higher flexibility to the experimental design.
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  • FCCS - Fluorescence Cross-Correlation Spectroscopy
    Science Lab Topic: Quantitative Fluorescence

    FCCS - Fluorescence Cross-Correlation Spectroscopy

    FCCS (Fluorescence Cross-Correlation Spectroscopy) can be measured using the Leica TCS SP8 FCS system. Similar to FCS , it analyzes fluorescence intensity fluctuations derived from a small…
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  • FRET with FLIM
    Science Lab Topic: Live-Cell Imaging

    FRET with FLIM

    FLIM combines lifetime measurements with imaging: lifetimes obtained for each image pixel are color-coded to produce additional image contrast. Thus, FLIM delivers information about the spatial…
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