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  • Live Cell Imaging

    Shifting perspective from single microscope components to a full working live cell imaging solution, Leica Microsystems integrates microscope, LAS X imaging software, cameras, and dedicated…
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  • Science Lab Topic: Quantitative Fluorescence

    Development of fluorescence lifetime imaging microscopy (FLIM) and its relevance for functional imaging

    Prof. Ammasi Periasamy, Director, Keck Center for Cellular Imaging, University of Virginia, was interviewed by Dr. Giulia Ossato, Product Manager functional imaging, during Leica Microsystems Meets…
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  • Science Lab Topic: Quantitative Fluorescence

    How FRET/FLIM Biosensors and Lifetime Imaging Helped Identify the Importance of Zinc for Human B-Cell Development

    In her latest studies, Dr. Mukta Deobagkar-Lele investigated the role of ZIP7, a zinc transporter protein, in modulating B cell activity and its effects on human immunity. During an interview with Dr.…
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  • Science Lab Topic: Quantitative Fluorescence

    Phasor Analysis for FLIM (Fluorescence Lifetime Imaging Microscopy)

    The Phasor analysis approach to analyze fluorescence lifetime does not require any fitting. Phasor FLIM (fluorescence lifetime imaging microscopy) provides a 2D graphical view of lifetime…
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  • primary murine pre B cells expressing the eCALWY-6 Zn2+
    Science Lab Topic: Quantitative Fluorescence

    Researchers use Functional Imaging to Elucidate the Role of Cellular Zn²⁺ in Human Immunity

    An international consortium of researchers has found a new mutation in a gene harboring a reticulum-to-cytoplasm zinc transporter. The gene is called ZIP7 and it is responsible for the early onset of…
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  • Science Lab Topic: Quantitative Fluorescence

    Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope

    Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by…
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  • Science Lab Topic: Super-Resolution

    Free Webinar On-Demand: Super-resolved STED spectroscopy

    Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules.
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  • Science Lab Topic: Quantitative Fluorescence

    FLIM FRET and Biosensors: Versatile Tools for Biomedical Research

    Fluorescence Lifetime Imaging (FLIM) in combination with Förster Resonance Energy Transfer (FRET) has proven to be very beneficial for investigations in biomedical research for a wide range of…
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  • Science Lab Topic: Quantitative Fluorescence

    Lifetime – a Proper Alternative

    „Way too complicated!“ - the notorious feedback when it comes to fluorescence lifetime measurements. This will change now! New technologies and new concepts for data evaluation, all implemented in…
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  • Science Lab Topic: Quantitative Fluorescence

    FCS - Fluorescence Correlation Spectroscopy

    FCS is a fluorescence-based measurement method. Fluorescent molecules passing through a strongly focused, fixed laser beam are excited for fluorescence emission. After passing a confocal pinhole, the…
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  • Science Lab Topic: Quantitative Fluorescence

    FLIM FRET - Förster Resonance Energy Transfer

    A typical application of FLIM is FLIM-FRET. FRET is a well-established technique to study molecular interactions. It scrutinizes protein binding and estimates intermolecular distances on an Angström…
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  • Science Lab Topic: Quantitative Fluorescence

    FLCS - Fluorescence Lifetime Correlation Spectroscopy

    Essentially, FCS can be performed with a continuous-wave laser. Using pulsed lasers allows even more sophisticated analysis possibilities, such as time-gated FCS or Fluorescence Lifetime Correlation…
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  • Science Lab Topic: Quantitative Fluorescence

    FLIM - Fluorescence Lifetime Imaging

    The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
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  • Science Lab Topic: Quantitative Fluorescence

    SP FLIM - Spectral Fluorescence Lifetime Imaging

    The SP8 FALCON is the ideal tool for spectral FLIM detection. No emission filters in front of the FLIM detectors are necessary. This grants a much higher flexibility to the experimental design.
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  • Science Lab Topic: Quantitative Fluorescence

    FCCS - Fluorescence Cross-Correlation Spectroscopy

    FCCS (Fluorescence Cross-Correlation Spectroscopy) can be measured using the Leica TCS SP8 FCS system. Similar to FCS , it analyzes fluorescence intensity fluctuations derived from a small…
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  • Science Lab Topic: Live-Cell Imaging

    FRET with FLIM

    FLIM combines lifetime measurements with imaging: lifetimes obtained for each image pixel are color-coded to produce additional image contrast. Thus, FLIM delivers information about the spatial…
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