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Confocal Technology

Technology helps to overcome bottlenecks in imaging for optimal performance

Leica Microsystems confocal instruments have been designed with an overall goal in mind, such as sensitivity or speed. Many innovations of the system complement each other to reinforce each other’s strength. Thus we create unique performance of our confocal systems as a whole.

Learn more about the components of the Leica TCS SP8

Leica Confocal Scanner

FOV Scanner

  • See full specimen in one shot (time saved on stitching)
  • Increased time resolution
  • Higher throughput in screening experiments
  • More about FOV Scanner

Galvo Flow (3D stacking without inertia)

  • Vibration-free, continuous movement
  • Real-time xz-slices
  • Faster 4D imaging
  • Ideally complements rapid scanning with Tandem Scanner
  • More about Galvo Flow

Tandem Scanner (FOV and resonant scanner combined)

Leica HyD™ (Hybrid photo detector)

  • Reduced light dosage improves cell viability
  • Ideal for high-speed imaging
  • Descanned or non-descanned detection
  • Quantitative through single photon counting (optional)
  • More about HyD

Visible Light Lasers

Solid State Lasers

  • Supply unit with small footprint
  • Flexible combinations of excitation lasers
  • Ideal for FRET with 448/514 nm and 488/552 nm pairs
  • More about lasers

White Light Laser

  • LightGate technology for higher image contrast
  • Freely tuneable excitation
    from 470 to 670 nm (1 nm steps)
  • Up to 8 fully tunable laser lines simultaneously out over 3 trillion unique combinations
  • Comprehensive spectroscopy with 2D excitation-emission scans
  • Pulsed excitation source for FLIM and gSTED
  • Develop new imaging strategies with full spectral freedom

Gas Lasers

  • Most flexible choice of laser lines
  • Atomic energy transitions for monochromatic light
  • Monochromatic light source for ideal spectral separation
  • Ideal for multi-color imaging
    with > 4 colors

Intermediate Optics

LIAchroics: Low incident angle dichroic beam splitters

  • High suppression of excitation light for high contrast
  • Custom designed low incident angle dichroics
  • Cost efficient beam splitting
  • More about Beam Splitters

Square Pinhole

SP Detector (Spectral Detector)

  • Gapless emission bands for faithful rendition of fluorescence spectra
  • Multi-band spectrophotometer
  • Prism-based dispersion for highest efficiency
  • Free from photon waste
  • More about SP Detector

AOBS: Acousto-optical beam splitter

  • Programmable beam splitter provides the highest flexibility
  • Support of White Light Laser (WLL)
  • Completely transparent for unhindered spectroscopy
  • Steep cutoff for high photon efficiency
  • Increased sample viability with low power of excitation laser
  • Switching in microseconds for fast multi-color kinetics
  • Be quantitative with constant laser power using Setlight

Objectives and Scan Optics

CS2 objectives

  • Improved color correction throughout the whole field of view
  • Perfect VIS – 405 overlay for best colocalization results
  • More about objectives

König-Rotator: Optical scan field rotation

  • Perfect image geometry independent of rotation angle
  • High transmission for minimal losses
  • Interactivity due to direct access by control panel

IRAPO objectives (dedicated apochromats for MP and OPO imaging)

  • High transmission in visible and infrared for brighter images from multiphoton and CARS
  • Exceptional axial and lateral color correction in the infrared for multiphoton excitation of multiple fluorophores

Scan optics dedicated to specific application ranges

  • Optimal transmission for each application
  • Dedicated intermediate optics for maximal photon efficiency
  • HIVIS from 400 to 800 nm (reduced reflection by 150%)
  • VISIR from 400 to 1300 nm
  • UVIS from 350 to 800 nm