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Galvo Flow: Sweeping through live specimens in 4D

For the fastest recording speed in 4D experiments, the Leica SuperZ stage offers a unique z-stacking strategy. A galvanometric stage insert is used for rapid an precise z-positioning. However, as xy scanning speed increases especially with the Tandem Scanners, the SuperZ can become rate limiting for large stacks e.g. in multiphoton imaging. The reason are inertia effects caused by the discontinuous movement of the sample. When moving to a z-position the galvanometer has to accelerate and then decelerate again. To match the speed of the Tandem Scanner in z, the speed of the SuperZ has been increasing by galvo flow. By continuously propelling the insert rather than in discreet steps, the system minimizes inertia. Thus, an otherwise rate-limiting repeated acceleration and deceleration is circumvented (Figure 1). The result are fast and clear 4D time series were the Tandem Scanner in xy direction and the SuperZ act self-synergistically.

Figure 1 In order to minimize inertia effects with galvo flow the SuperZ insert is continuously propelled. This results in accerlated data recording of 4D time series; especially involving large stacks in tissue.