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Confocal Microscopes

Confocal microscopes from Leica Microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in three-dimensional imaging and exact examination of subcellular structures and dynamic processes.

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STELLARIS - Confocal Microscope Platform

STELLARIS. GET CLOSER TO THE TRUTH.

With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.

SP8 DIVE

The first multiphoton microscope with spectrally tunable detection.

Maximum information from each specimen with LIGHTNING

LIGHTNING

The maximum of information from each specimen

Leica TCS SP8 CARS Confocal Microscope-Label Free Imaging

Leica TCS SP8 CARS

Label-free imaging of native specimens

Leica TCS SPE with Leica DMi8 and climate chamber

Leica TCS SPE

The personal spectral confocal for daily research

Smart monitoring and service for scientific instruments RemoteCare

RemoteCare

Smart monitoring and service option for your microscope

Featured image

Mouse embryo mosaic image

High resolution mouse embryo mosaic image of 722 tiles containing 190 Megapixels. FLIM data fitted with four characteristic fluorescence lifetimes, color coded. Acquisition: 1:23 h. Analysis: 1:00 h

STELLARIS FALCON

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Confocal Microscopes Latest News

Image Gallery: THUNDER Imager

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using…

Leica Science Lab

Explore Innovative Techniques to Separate Fluorophores with Overlapping Spectra

In this article we explore several strategies you can take to improve the separation of fluorophores and increase the number of fluorescent probes you…

Leica Science Lab

Add an Extra Dimension to Confocal Results with TauSense

Leica Microsystems’ TauSense technology is a set of imaging modes based on fluorescence lifetime. Found at the core of the STELLARIS confocal…

Leica Science Lab

How to Uncover Hidden Dimensions in Research with Lifetime Imaging

The vast majority of imaging experiments measure fluorescence intensity but there is another key property of fluorescence—its lifetime, which is…

Leica Science Lab

Learn how to Remove Autofluorescence from your Confocal Images

Autofluorescence can significantly reduce what you can see in a confocal experiment. This article explores causes of autofluorescence as well as…

Leica Science Lab

Interview with Magali Mondin on THUNDER Imagers

Magali Mondin, an engineer at the BIC (Bordeaux Imaging Center) in France, describes her experience using a THUNDER Imager in this interview. She had…

Leica Science Lab

Multiphoton Microscopy Publication List

Multiphoton Microscopy is an advanced technique for imaging thick samples. Applications range from the visualization of the complex architecture of…

Leica Science Lab

How to prepare samples for Stimulated Raman Scattering

Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows…

Leica Science Lab

Principles of Multiphoton Microscopy for Deep Tissue Imaging

Basics of multiphoton microscopy. This tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy…

Leica Science Lab

Step by Step Guide to the Molecular Basics of CARS Microscopy

CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic…

Leica Science Lab

About Confocal

Confocal Laser Scanning Microscopy (CLSM) is one of a series of methods to generate slices from microscopic samples by means of optics. The sample stays intact, and the slicing may be repeated many times. True Confocal Scanning (TCS) is a technique, where only a single, diffraction limited spot is illuminated and observed at a time. The benefit of confocal imaging is a dramatically increased contrast by removal of out-of-focus haze. Z-sequences of optical slices (3D image stacks) are sources for subsequent rendering as anaglyphes, depth-coded maps or 3D movies. TCS is also very well compatible with multi-fluorescence imaging, time-lapse imaging, FLIMFRAP and  FCS  measurements – plus a whole world of spectral applications.

Useful Links

Communities and Web Sources

www.researchgate.net/Social network for scientists

www.lsoft.com/scripts/wl.exe?SL1=CONFOCALMICROSCOPY&H=LISTS.UMN.EDUConfocal Microscopy Mailing List, University of Minnesota

www.ibiology.org/Teaching tools, video lectures on biology and microscopy

www.ibiology.org/Teaching tools, video lectures on biology and microscopy

bitesizebio.comOnline magazine and community for molecular and cell biology researchers

www.somersault1824.comResource for high-end scientific illustrations, images and animations

Search Engines and Data Bases

www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells

harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins

www.gopubmed.comSearch interface for pubmed

en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines

scholar.google.comBeta of Google's search engine for scientific article abstracts

Journals

www.doaj.org/Directory of open access journals

emboj.embopress.org/The EMBO Journal

www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal

www.sciencemag.org/Science

www.nature.com/Nature

www.cell.com/Biweekly publication of exceptional research articles

jcs.biologists.org/Journal of Cell Science

dev.biologists.org/Development

jeb.biologists.org/The Journal of Experimental Biology

dmm.biologists.org/DMM Disease Models & Mechanisms

www.biotechniques.com/International Journal of Life Science Methods

www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics

spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics

onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics

www.plosone.org/home.actionInternational, peer-reviewed, open-access, online publication

rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal

www.microscopy-analysis.com/International Journal for microscopists

Organizations

www.microscopy.org/Microscopy Society of America

www.eurmicsoc.org/European Microscopy Society

www.rms.org.uk/Royal Microscopical Society

www.ascb.org/ASCB American Society of Cell Biology

www.biologists.com/cob_activities.htmlthe company of biologists

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