Confocal Microscopes
Contact a local imaging specialist for expert advice on the right Confocal Microscopy system for your needs.
Confocal Microscopes
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STELLARIS
With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.
STELLARIS DLS
Discover new applications by combining confocal and light-sheet microscopy
STELLARIS 8 FALCON
The contrast is clear. Lifetime imaging in an instant.
STELLARIS STED & STELLARIS 8 STED
STELLARIS brings the full power and potential of STED in a fully integrated system.
STELLARIS 8 DIVE
STELLARIS 8 DIVE (Deep In Vivo Explorer) is a multiphoton microscope with spectrally tunable detection.
Leica TCS SP8 CARS
Label-free imaging of native specimens
RemoteCare
Smart monitoring and service option for your microscope
News
Plant Cell Development and Morphogenesis
The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence…
Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using…
Laser Microdissection Publication List
This list presents current publications in the major application fields for laser microdissection and is updated monthly.
A New World of Confocal Applications with the Next Generation White Light Lasers
As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the…
Immediate Breast Reconstruction: Benefits of GLOW800 Augmented Reality
Dr. Harold Chatel is a Plastic and Reconstructive Surgeon in a leading hospital in Paris, France. One of his areas of expertise is oncological…
Oncological Reconstructive Surgery with the Leica M530 OHX Microscope
Precision is essential in oncological reconstructive surgery, in particular when it relies on free flap techniques. Microsurgical microscopes provide…
Oncological Reconstructive Surgery: Why Use a Microscope
Recent advances in microsurgery are enhancing breast reconstruction for oncology patients, allowing both functional and aesthetic rehabilitation. More…
Oncological Reconstructive Surgery: Benefits of Microscope & Integrated Vascular Fluorescence Use
Oncological reconstructive surgery requires high precision and attention to details. It can pose a number of challenges: long operating time, flap…
About Confocal
Confocal Laser Scanning Microscopy (CLSM) is one of a series of methods to generate slices from microscopic samples by means of optics. The sample stays intact, and the slicing may be repeated many times. True Confocal Scanning (TCS) is a technique, where only a single, diffraction limited spot is illuminated and observed at a time. The benefit of confocal imaging is a dramatically increased contrast by removal of out-of-focus haze. Z-sequences of optical slices (3D image stacks) are sources for subsequent rendering as anaglyphes, depth-coded maps or 3D movies. TCS is also very well compatible with multi-fluorescence imaging, time-lapse imaging, FLIM, FRAP and FCS measurements – plus a whole world of spectral applications.
Latest Articles
Nobel Prize 2012 in Chemistry for Studies of G-Protein-Coupled Receptors
The Royal Swedish Academy of Sciences has decided to award the Nobel Prize in Chemistry for 2012 to Robert J. Lefkowitz and Brian K. Kobilka for studies of G-protein–coupled receptors.Read article
Nobel Prize 2012 in Physiology or Medicine for Stem Cell Research
The Nobel Prize recognizes two scientists who discovered that mature, specialised cells can be reprogrammed to become immature cells capable of developing into all tissues of the body. Their findings…Read article
Label-free FLIM
Many biological samples exhibit autofluorescence. Its often broad spectra can interfere with fluorescent labeling strategies. This application letter demonstrates how autofluorescence can serve as an…Read article
FRET with FLIM
FLIM combines lifetime measurements with imaging: lifetimes obtained for each image pixel are color-coded to produce additional image contrast. Thus, FLIM delivers information about the spatial…Read article
Fluorescence Correlation Spectroscopy
Fluorescence correlation spectroscopy ( FCS ) measures fluctuations of fluorescence intensity in a sub-femtolitre volume to detect such parameters as the diffusion time, number of molecules or dark…Read article
White Light Laser
The perfect light source for confocal microscopes in biomedical applications has sufficient intensity, tunable color and is pulsed for use in lifetime fluorescence. Furthermore, it should offer means…Read article
Order versus Disorder
In modern biomaterial design the generation of an environment mimicking some of the extracellular matrix features is envisaged to support molecular cross-talk between cells and scaffolds during tissue…Read article
Scientific Illustration and Animation for Research Scientists
One of the crucial tasks of a research scientist is reporting and communicating about his work. This is vital for cutting edge research; it is crucial to gain insights from other experts, to get a…Read article
Live-cell Imaging Techniques
The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focusing on dynamic…Read article
How to Study Protein Recruitment to DNA Lesions by a Combination of UV Laser and White Light Laser
Understanding how DNA lesions are optimally repaired is of functional significance, especially from the view of genome karyotype stability.Read article
The New Repository on the Block
The need for data validation and accessibility has never been greater than it is today. We are inundated with information from a multitude of resources, but how can we easily evaluate the accuracy of…Read article
FLCS – Advances in Fluorescence Correlation Spectroscopy
The characterization of substances at the single molecule level has become part of the standard repertoire of scientific research institutes. One of the most common methods is Fluorescence Correlation…Read article
Beam Splitting
Fluorescence Microscopy usually employs incident light illumination. This requires a device that directs the light for illumination into the sample and transmits the light emitted by the sample to the…Read article
Spectral Imaging
To separate fractions of the emission for recording channels that reproduce the emission of individual fluorochromes, it is necessary to spatially disperse the emission spectrally. This is possible by…Read article
Confocal Excitation
Fluorescence excitation needs specifically colored light. In confocal microscopy, multiline lasers or laser batteries are classically used. This requires devices that pick the requested lines fitting…Read article
Fluorescence Recovery after Photobleaching (FRAP) and its Offspring
FRAP (Fluorescence recovery after photobleaching) can be used to study cellular protein dynamics: For visualization the protein of interest is fused to a fluorescent protein or a fluorescent dye. A…Read article
Förster Resonance Energy Transfer (FRET)
The Förster Resonance Energy Transfer (FRET) phenomenon offers techniques that allow studies of interactions in dimensions below the optical resolution limit. FRET describes the transfer of the energy…Read article
Mosaic Images
Confocal laser scanning microscopes are widely used to create highly resolved 3D images of cells, subcellular structures and even single molecules. Still, an increasing number of scientists are…Read article
Brighter Fluorescence by Resonant Scanning
Fast True Confocal Scanning reduces photobleaching and increases the fluorescence yield at identical acquisition times. The long-lasting triplet state (or any other “dark state”) is less populated…Read article
Find the Needle in the Haystack
The obvious has been explored. These days, biologists strive to identify and analyze hidden and rare events. The task is tackled by automatically screening large numbers of objects – typically growing…Read article
Pinhole Controls Optical Slicing
True confocal scanning microscopes (TCS) use a variable detection pinhole. Good optical sectioning tries to use just the inner core of the PSF.Read article
Confocal Optical Section Thickness
Confocal microscopes are employed to optically slice comparably thick samples.Read article
Lasers for Confocal
True confocal scanning microscopy (TCS) requires bright diffraction-limited illumination.Read article
Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
Organotypic cultures of neuronal tissue were first introduced by Hogue in 1947 and have constituted a major breakthrough in the field of neuroscience. Since then, the technique was developed further…Read article
Confocal Microscopy - Optical Path
“Confocal Microscopy” refers to a particular optical microscope that allows recording optical sections. Optical sectioning is achieved in a confocal system by illuminating and observing a single…Read article
Choose Your Excitation Wavelength
Although time correlated single photon counting (TCSPC) is the method of choice for fluorescence lifetime quantification, it requires dedicated instrumentation including a pulsed laser source, a…Read article
Deep Tissue Imaging
Developmental biology using Multiphoton microscopy with OPO. To gain new insight into the fundamental control of cell response to physical changes and to study the dynamics and roles of biological…Read article
What is an OPO?
Multiphoton microscopy with OPO: imaging with excitation wavelengths up to 1.300 nm. Because light scattering is dependent on the wavelength, better tissue penetration can be achieved by using…Read article
Exploring the Concert of Neuronal Activities
Brain research using Confocal and Multiphoton Microscopy. Using imaging techniques such as confocal and two-photon microscopy, neuronal dendritic arborization of neurons and their synaptic…Read article
CARS and Confocal
The most important drawback of single-photon and multiphoton confocal microscopy is the need to label the specimen. CARS (Coherent Anti-Stokes Raman Spectroscopy) addresses this issue because it is…Read article
Useful Links
Communities and Web Sources
www.researchgate.net/Social network for scientists
www.lsoft.com/scripts/wl.exe?SL1=CONFOCALMICROSCOPY&H=LISTS.UMN.EDUConfocal Microscopy Mailing List, University of Minnesota
www.ibiology.org/Teaching tools, video lectures on biology and microscopy
www.ibiology.org/Teaching tools, video lectures on biology and microscopy
bitesizebio.comOnline magazine and community for molecular and cell biology researchers
www.somersault1824.comResource for high-end scientific illustrations, images and animations
Search Engines and Data Bases
www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells
harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins
www.gopubmed.comSearch interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines
scholar.google.comBeta of Google's search engine for scientific article abstracts
Journals
www.doaj.org/Directory of open access journals
emboj.embopress.org/The EMBO Journal
www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal
www.sciencemag.org/Science
www.nature.com/Nature
www.cell.com/Biweekly publication of exceptional research articles
jcs.biologists.org/Journal of Cell Science
dev.biologists.org/Development
jeb.biologists.org/The Journal of Experimental Biology
dmm.biologists.org/DMM Disease Models & Mechanisms
www.biotechniques.com/International Journal of Life Science Methods
www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics
spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics
onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics
www.plosone.org/home.actionInternational, peer-reviewed, open-access, online publication
rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal
www.microscopy-analysis.com/International Journal for microscopists
Organizations
www.microscopy.org/Microscopy Society of America
www.eurmicsoc.org/European Microscopy Society
www.rms.org.uk/Royal Microscopical Society
www.ascb.org/ASCB American Society of Cell Biology
www.biologists.com/cob_activities.htmlthe company of biologists
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