Leica LMD6 & LMD7 Laser Microdissection Microscopes

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

DNA Research

Characterization of the evolution trajectory and immune profiling of new histologic patterns in lung adenocarcinoma
Song M, Xie H, Liu W, Zhao M, Deng Z, Zhang L, Liu H, Huang Y, Li H, Ren Y, Chen C
J Gene Med. 2022 Oct 4:e3455. doi: 10.1002/jgm.3455.

Ordered and deterministic cancer genome evolution after p53 loss.
Baslan T, Morris JP 4th, Zhao Z, Reyes J, Ho YJ, Tsanov KM, Bermeo J, Tian S, Zhang S, Askan G, Yavas A, Lecomte N, Erakky A, Varghese AM, Zhang A, Kendall J, Ghiban E, Chorbadjiev L, Wu J, Dimitrova N, Chadalavada K, Nanjangud GJ, Bandlamudi C, Gong Y, Donoghue MTA, Socci ND, Krasnitz A, Notta F, Leach SD, Iacobuzio-Donahue CA, Lowe SW
Nature. 2022 Aug;608(7924):795-802. doi: 10.1038/s41586-022-05082-5.

Anatomical Laser Microdissection of the Ileum Reveals mtDNA Depletion Recovery in A Mitochondrial Neuro-Gastrointestinal Encephalomyopathy (MNGIE) Patient Receiving Liver Transplant
Boschetti E, Caporali L, D'Angelo R, Malagelada C, Accarino A, Dotti MT, Costa R, Cenacchi G, Pironi L, Rinaldi R, Stanghellini V, Ratti S, Manzoli L, Carelli V, De Giorgio R
Int J Mol Sci. 2022 Aug 8;23(15):8792. doi: 10.3390/ijms23158792.

RNA Research

The cellulose–lignin balance affects the twisted growth of Yunnan pine trunk
Cui K, Liu Z, Li J, Gao C, and Miao Y
Authorea Preprints, 10 Oct 2022 

RNA-seq profiling reveals different pathways between remodelled vessels and myocardium in Hypertrophic Cardiomyopathy
Pisano A, Le Pera L, Carletti R, Cerbelli B, Pignataro MG, Pernazza A, Ferre F, Lombardi M, Lazzeroni D, Olivotto I, Rimoldi OE, Foglieni C, Camici PG, d'Amati G
Microcirculation. 2022 Oct 5:e12790. doi: 10.1111/micc.12790.

mRNA-miRNA networks identify metabolic pathways associated to the anti-tumorigenic effect of thyroid hormone on preneoplastic nodules and hepatocellular carcinoma
Serra M, Pal R, Puliga E, Sulas P, Cabras L, Cusano R, Giordano S, Perra A, Columbano A and Kowalik MA
Front. Oncol. 12:941552. (2022), doi: 10.3389/fonc.2022.941552

Proteomics, Lipidomics, Metabolomics

Sex Differences in the Systemic and Local Immune Response of Pancreatic Cancer Patients
Ahmed A, Köhler S, Klotz R, Giese N, Hackert T, Springfield C, Jäger D, and Halama N
Cancers 2023, 15(6), 1815; doi.org/10.3390/cancers15061815

Target formation in muscle fibres indicates reinnervation - a proteomic study in muscle samples from peripheral neuropathies
Krause K, Eggers B, Uszkoreit J, Eulitz S, Rehmann R, Güttsches AK, Schreiner A, van der Ven PFM, Fürst DO, Marcus K, Vorgerd M, Kley RA
Neuropathol Appl Neurobiol. 2022 Sep 30:e12853. doi: 10.1111/nan.12853.

The addition of FAIMS increases targeted proteomics sensitivity from FFPE tumor biopsies
Sweet S, Chain D, Yu W, Martin P, Rebelatto M, Chambers A, Cecchi F, Kim YJ
Sci Rep. 2022 Aug 16;12(1):13876. doi: 10.1038/s41598-022-16358-1

Spatial quantitation of antibiotics in bone tissue compartments by laser-capture microdissection coupled with UHPLC-tandem mass spectrometry
Kaya F, Zimmerman MD, Antilus-Sainte R, Gengenbacher M, Carter CL, Dartois V
Anal Bioanal Chem. 2022 Aug 10. doi: 10.1007/s00216-022-04257-3.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

Target formation in muscle fibres indicates reinnervation - a proteomic study in muscle samples from peripheral neuropathies
Krause K, Eggers B, Uszkoreit J, Eulitz S, Rehmann R, Güttsches AK, Schreiner A, van der Ven PFM, Fürst DO, Marcus K, Vorgerd M, Kley RA
Neuropathol Appl Neurobiol. 2022 Sep 30:e12853. doi: 10.1111/nan.12853.

Ordered and deterministic cancer genome evolution after p53 loss.
Baslan T, Morris JP 4th, Zhao Z, Reyes J, Ho YJ, Tsanov KM, Bermeo J, Tian S, Zhang S, Askan G, Yavas A, Lecomte N, Erakky A, Varghese AM, Zhang A, Kendall J, Ghiban E, Chorbadjiev L, Wu J, Dimitrova N, Chadalavada K, Nanjangud GJ, Bandlamudi C, Gong Y, Donoghue MTA, Socci ND, Krasnitz A, Notta F, Leach SD, Iacobuzio-Donahue CA, Lowe SW
Nature. 2022 Aug;608(7924):795-802. doi: 10.1038/s41586-022-05082-5

Deciphering the dynamic niches and regeneration-associated transcriptional program of motoneurons following peripheral nerve injury
Zhang Y, Xu L, Li X, Chen Z, Chen J, Zhang T, Gu X, Yang J
iScience. 2022 Aug 11;25(9):104917. doi: 10.1016/j.isci.2022.104917.

Cancer Research

Characterization of the evolution trajectory and immune profiling of new histologic patterns in lung adenocarcinoma
Song M, Xie H, Liu W, Zhao M, Deng Z, Zhang L, Liu H, Huang Y, Li H, Ren Y, Chen C
J Gene Med. 2022 Oct 4:e3455. doi: 10.1002/jgm.3455.

mRNA-miRNA networks identify metabolic pathways associated to the anti-tumorigenic effect of thyroid hormone on preneoplastic nodules and hepatocellular carcinoma
Serra M, Pal R, Puliga E, Sulas P, Cabras L, Cusano R, Giordano S, Perra A, Columbano A and Kowalik MA
Front. Oncol. 12:941552. (2022), doi: 10.3389/fonc.2022.941552

A novel lncRNA MTAR1 promotes cancer development through IGF2BPs mediated post-transcriptional regulation of c-MYC
Gao Y, Jiang M, Guo F, Liu X, Zhang Q, Yang S, Yeung YT, Yang R, Wang K, Wu Q, Zhang D, Zhang C, Laster KV, Ge M, Nie W, Liu K, Dong Z
Oncogene. 2022 Sep 15. doi: 10.1038/s41388-022-02464-x.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Neurosciences

Endogenous oxytocin exerts anti-nociceptive and anti-inflammatory effects in rats
Nishimura H, Yoshimura M, Shimizu M, Sanada K, Sonoda S, Nishimura K, Baba K, Ikeda N, Motojima Y, Maruyama T, Nonaka Y, Baba R, Onaka T, Horishita T, Morimoto H, Yoshida Y, Kawasaki M, Sakai A, Muratani M, Conway-Campbell B, Lightman S, Ueta Y
Commun Biol. 2022 Sep 5;5(1):907. doi: 10.1038/s42003-022-03879-8.

Neuromedin U-deficient Rats do not Lose Body Weight or Food Intake
Yokogi K, Goto Y, Otsuka M, Ojima F, Kobayashi T, Tsuchiba Y, Takeuchi Y, Namba M, Kohno M, Tetsuka M, Takeuchi S, Matsuyama M, Aizawa S
Research Square, 16. Aug 2022, DOI: doi.org/10.21203/rs.3.rs-1907525/v1

Deciphering the dynamic niches and regeneration-associated transcriptional program of motoneurons following peripheral nerve injury
Zhang Y, Xu L, Li X, Chen Z, Chen J, Zhang T, Gu X, Yang J
iScience. 2022 Aug 11;25(9):104917. doi: 10.1016/j.isci.2022.104917.

Clinical/Pathology/Immunology Research Applications

RNA-seq profiling reveals different pathways between remodelled vessels and myocardium in Hypertrophic Cardiomyopathy
Pisano A, Le Pera L, Carletti R, Cerbelli B, Pignataro MG, Pernazza A, Ferre F, Lombardi M, Lazzeroni D, Olivotto I, Rimoldi OE, Foglieni C, Camici PG, d'Amati G
Microcirculation. 2022 Oct 5:e12790. doi: 10.1111/micc.12790.

Target formation in muscle fibres indicates reinnervation - a proteomic study in muscle samples from peripheral neuropathies
Krause K, Eggers B, Uszkoreit J, Eulitz S, Rehmann R, Güttsches AK, Schreiner A, van der Ven PFM, Fürst DO, Marcus K, Vorgerd M, Kley RA
Neuropathol Appl Neurobiol. 2022 Sep 30:e12853. doi: 10.1111/nan.12853.

Deletion of Tfam in Prx1-Cre expressing limb mesenchyme results in spontaneous bone fractures.
Yoshioka H, Komura S, Kuramitsu N, Goto A, Hasegawa T, Amizuka N, Ishimoto T, Ozasa R, Nakano T, Imai Y, Akiyama H
J Bone Miner Metab. 2022 Aug 10. doi: 10.1007/s00774-022-01354-2.

Developmental Research, Epigenetics  & Aging

TGF-β3 induces lactate production in Sertoli cell through inhibiting Notch pathway
Xu Y, Jiang S, Hu Y, Zhang Q, Su W
Andrology. 2022 Sep 4. doi: 10.1111/andr.13288.

Biomimetic Tubular Matrix Induces Periodontal Ligament Principal Fiber Formation and Inhibits Osteogenic Differentiation of Periodontal Ligament Stem Cells
Liang Y, Shakya A, Liu X
ACS Appl Mater Interfaces. 2022 Aug 7. doi: 10.1021/acsami.2c09420.

Slc12a8 in the lateral hypothalamus maintains energy metabolism and skeletal muscle functions during aging
Ito N, Takatsu A, Ito H, Koike Y, Yoshioka K, Kamei Y, Imai SI
Cell Rep. 2022 Jul 26;40(4):111131. doi: 10.1016/j.celrep.2022.111131.

Plant Research

The cellulose–lignin balance affects the twisted growth of Yunnan pine trunk
Cui K, Liu Z, Li J, Gao C, and Miao Y
Authorea Preprints, 10 Oct 2022, 

Natural variation of DROT1 confers drought adaptation in upland rice
Sun X, Xiong H, Jiang C, Zhang D, Yang Z, Huang Y, Zhu W, Ma S, Duan J, Wang X, Liu W, Guo H, Li G, Qi J, Liang C, Zhang Z, Li J, Zhang H, Han L, Zhou Y, Peng Y, Li Z.
Nat Commun. 2022 Jul 23;13(1):4265. doi: 10.1038/s41467-022-31844-w.

Plant-Fungal Interactions: Laser Microdissection as a Tool to Study Cell Specificity
Balestrini R, Sillo F
Methods Mol Biol. 2022;2536:369-380. doi: 10.1007/978-1-0716-2517-0_20.

Forensics

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J:
BioMed Research International, Volume 2021 | Article ID 7269237

Application and utility of alternative methods in isolation of pure cells from forensic biological mixtures in modern-day: a review
Prajapati S, Rajmane P, Jayakrishna P, Nair MS, Kshirsagar P and Meshram M:
JFSR – Journal of Forensic Science and Research, Aug 2021, ISSN 2575-0186

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics: 
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Improved Spatial Resolution of Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) Mass Spectrometry Imaging (MSI) Using a Reflective Objective
Joignant AN, Bai H, Manni JG Sr, Muddiman DC 
Rapid Commun Mass Spectrom. 2022 Sep 4:e9392. doi: 10.1002/rcm.9392.

Profiling cell-type specific gene expression in post-mortem human brain samples through laser capture microdissection
Almeida D, Turecki G
Methods. 2022 Sep 2:S1046-2023(22)00196-7. doi: 10.1016/j.ymeth.2022.08.013.

Hepatic cytochrome P450 8B1 and cholic acid potentiate intestinal epithelial injury in colitis by suppressing intestinal stem cell renewal
Chen L, Jiao T, Liu W, Luo Y, Wang J, Guo X, Tong X, Lin Z, Sun C, Wang K, He Y, Zhang Y, Xu H, Wang J, Zuo J, Ding Q, He S, Gonzalez FJ, Xie C
Cell Stem Cell. 2022 Sep 1;29(9):1366-1381.e9. doi: 10.1016/j.stem.2022.08.008.

Live Cell Culture

Heterotypic cell-in-cell structures between cancer and NK cells are associated with enhanced anticancer drug resistance
Choe YJ, Min JY, Lee H, Lee SY, Kwon J, Kim HJ, Lee J, Kim HM, Park HS, Cho MY, Hyun JY, Kim HM, Chung YH, Ha SK, Jeong HG, Choi I, Kim TD, Hong KS, and Han EH
iScience 25, 105017 September 16, 2022

Activation of CD44 signaling in leader cells induced by tumor-associated macrophages drives collective detachment in luminal breast carcinomas
Gao F, Zhang G, Liu Y, He Y, Sheng Y, Sun X, Du Y, Yang C
Cell Death Dis. 2022 Jun 9;13(6):540. doi: 10.1038/s41419-022-04986-4.

Transcriptome Analysis of Schwann Cells at Various Stages of Myelination Implicates Chromatin Regulator Sin3A in Control of Myelination Identity
Zhang B, Su W, Hu J, Xu J, Askar P, Bao S, Zhou S, Chen G, Gu Y
Neurosci Bull. 2022 Apr 10. doi: 10.1007/s12264-022-00850-9.