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Leica LMD6 & LMD7 Laser Microdissection Microscopes
Dissection Perfection
Dedicated Laser Microdissection Objectives
Leica Microsystems offers a portfolio of dry objectives dedicated for Leica LMD systems. These special LMD objectives feature the highest possible UV-transmission and outstanding imaging performance – the Leica SmartCut series (5x–150x).
| Objective | Mag. | NA | WD (mm) | BF, POL | FL | DIC, PH | LMD | CI | SOV |
| HCX PL FLUOTAR** | 1.25x | 0.04 | 3.7 | + | + | – | – | – | +++ |
| PLAN** | 4x | 0.1 | 26.2 | + | + | – | + | ++ | ++ |
| UVI | 5x | 0.12 | 11.7 | + | + | – | +++ | ++ | ++ |
| HI PLAN | 6.3x | 0.13 | 12.8 | + | + | – | +++ | ++ | ++ |
| HCX PL FLUOTAR | 10x | 0.3 | 11.0 | + | + | + | +++ | + | + |
| UVI | 10x | 0.25 | 2.9 | + | + | + | +++ | – | + |
| HCX PL FLUOTAR | 20x | 0.4 | 6.9 | + | + | + | +++ | + | + |
| HCX PL FLUOTAR | 40x | 0.6 | 3.3–1.9 | + | + | + | +++ | – | – |
| HCX PL FLUOTAR | 63x | 0.7 | 2.6–1.8 | + | + | + | +++ | – | – |
| HCX PL FLUOTAR | 150x | 0.9 | 0.25 | + | + | + | +++ | – | – |
Mag. – magnification; NA – numerical aperture; WD – working distance; BF – brightfield; POL – polarized light; FL – fluorescence; DIC – differential interference contrast; PH – phase contrast; LMD – laser microdissection; CI – cap inspection; SOV – specimen overview; + = suitable; – = not suitable; ++ = dedicated for; +++ = most suitable
** Additionally recommended objectives
Technical Specifications
| Dissection | Dissection and Collection Unit Based on Scanning Stage | Dissection and Collection Unit Based on Motorized Stage | Dissection and Collection Unit Based on Scanning Stage LMT350 |
| Specimen Collection | Contact- and contamination-free | ||
| Dissection modes | Draw & Cut Move & Cut (direct online cutting) PtoP (point-to-point) Draw & Scan (dot dissection scan) LMD screw (cut subsequently in Z-direction) Shadow Cuts (parallel draw and dissection) | ||
| Serial section cutting | Yes | No | Yes |
| AVC+ basic AVC+ professional | Yes Yes | Yes Not recommended | Yes |
| Universal holder | Yes | No | No |
| Stage repeatability | +/- 1 µm | +/- 10 µm | <=+/- 0.5 µm |
| Stage max. travel speed | 20 mm/s | 200 mm/s | |
| Stage drive | Ball bearing screw | Rack/ pinion | Linear motor drives |
| Holders | 3x standard slides (25 mm x 76 mm) Optional: 1x big slide (50 mm x 76 mm) or 2x standard slides (25 mm x 76 mm) Petri dish (50 mm) 18-well slide (stack) | 1x standard slide (25 mm x 76 mm) Optional: 1x big slide (50 mm x 76 mm) or 2x standard slides (25 mm x 76 mm) Petri dish (50 mm) | 4x standard slides (25 mm x 76 mm) Optional (combinations possible): 1x big slide (50 mm x 76 mm) 2x Petri dishes (50 mm) 2x 18-well Ibidi slide stacks |
| Collectors | 4x 0.2 ml standard PCR tubes 4x 0.5 ml standard PCR tubes Petri dish (50 mm) Optional: 2x 8-well strips building up a 96-well plate Height adjustable universal collector (for multi-well slides, 8-strips, 8-strip tube caps, ...) 48-well collector (6x8-strip tube caps) (2x for a full 96-well plate) | 4x 0.2 ml standard PCR tubes 4x 0.5 ml standard PCR tubes Optional: 1x 8-well strips building up a 96-well plate | 4x 0.2 ml standard PCR tubes 4x 0.5 ml standard PCR tubes 2x Petri dishes (50 mm) Optional: 96-PCR-well plate (non-skirted or semi-skirted) 96-LCC-well plate 2x 18-well Ibidi slides 1 x 8-strip, 1 x 12-strip 1.5 ml tube (with or without filling) |
| Power supply | CTR6/CTR6 LED | Dual SP box | |
| Laser | Leica LMD7 | Leica LMD6 | |||
| Type | Diode pumped, solid state | Diode pumped, solid state | |||
| Wavelength | 349 nm | 355 nm | |||
| Average pulse energy | 120 µJ | 70 µJ | |||
| Repetition rate | 10 – 5000 Hz (free adjustable) | 80 Hz (fix) | |||
| Laser aperture control | Yes, continuously adjustable | ||||
| Free intensity control | 1-100% | ||||
| UV offset freely adjustable and saved for each objective | Yes | ||||
| Laser beam movement | Via optics | ||||
| Microscope stand (fully automated DM6 B) | |||||
| Transmitted light (TL) axis | Contrast methods | BF, optional PH, DF, POL, DIC (fully automated) | |||
| Illumination | LED (PMax = 15 W ) Lamphousing | ||||
| Automation | Automated illumination manager Automated contrast manager Constant color intensity control (CCIC) for Halogen TL | ||||
| Condenser | Condenser head S28, 0.55 NA Motorized 7x condenser disk Motorized polarizer | ||||
| Fluorescence axis | Filter cube turret | Motorized 5x or 8x | |||
| Automation | Fluorescence intensity manager (FIM) for brightness adjustment Circular and rectangular field diaphragms for eyepiece or camera viewing Internal filter wheel and motorized Excitation Manager | ||||
| Illumination | Leica EL6000 (120 W metal halide) or 100 W HBO | ||||
| Cubes (individual filters available upon request) | Special cubes (size k) for simultaneous fluorescence and laser application | ||||
| LMD-BGR LMD-GFP band pass LMD-GFP long pass LMD-Cy3 LMD-DAPI | LMD-Alexa594 LMD-CFP LMD-GFP/Cy3 LMD-YFP LMD-Cy5 | ||||
| Operation | Focus | Motorized: – 5 electronic ratios – Includes parfocal function Memory function for two z-positions | |||
| Objective turret | Motorized 7x M25 thread including dry and immersion modes | ||||
| Controls | 6 programmable function buttons | ||||
| Leica SmartMove Controls for z (focus) movement and x, y (stage) movement 4 programmable function buttons | |||||
| Optional Leica STP8000 Controls for z (coarse and fine focus) and x, y (stage) movement 11 programmable function buttons Touchscreen with information and control panels | |||||
| Stand | Display | With integrated touchscreen Leica SmartTouch | |||
| Interfaces | 2 x USB 2.0, 2 x I2C | ||||
| Dimensions | With scanning stage: 649.6 mm height, 512.0 mm width, 596.5 mm depth | ||||
| System software (Operating System Microsoft® Windows® 7) | |||||
| Package includes (selected features) | Dissection | Automated collection devices and positioning of PCR tubes Fully automated inspection mode Multi-cutting over entire slide Save and load drawn shapes | |||
| User guidance | Workflow based graphical user interface Free scaling of drawn shapes Save user profiles Overview images in BF and Fluorescence | ||||
| Control | Full laser control Control software of microscope stand Laser and illumination settings are linked to objectives | ||||
| Interfaces | Export shape list data for Microsoft Excel or OpenOffice | ||||
| Optional software packages | Automated vision control (AVC+ basic) for automated cell recognition within field of view only Automated vision control (AVC+ professional) for fully or semi automated cell recognition over freely defined area Integrated database to transfer relevant data (laser, microscope and camera; database as optional) | ||||
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