Leica LMD Systems Laser Microdissection Microscopes

This curated list features recent scientific publications that have employed Leica’s LMD6 and LMD7 laser microdissection systems in key areas such as cancer research, neuroscience, molecular pathology, forensics, and spatial omics. It is updated regularly to reflect the latest scientific publications utilizing laser microdissection applications.

Explore the full list below to see how Leica LMD systems are enabling scientific success across the globe.

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Single Cell Applications

A molecularly defined neural circuit for cold defense revealed by targeted single-nucleus transcriptomic mapping
Wu Y, Liu Z, Xiong T, Fan L, Chen Y, Duan X, Liu Y, Jing H, Liu X, Zhu Q, Zhang S, Liang W, Lu X, Ji SJ, Chen W, Zhu Y, Zhai J, Song K
Research Square, May 27th, 2026

Expanding the landscape of tRNA pathogenic variants in mitochondrial DNA
Siragusa SL, Blando S, Fiorini C, Pasti AP, Ormanbekova D, Casadei F, Morandi L, Pegoraro E, Liguori R, Carelli V, La Morgia C, Caporali L, Valentino ML
Brain Communications, fcag178, 20 May 2026, doi.org/10.1093/braincomms/fcag178

Spatial Single-Cell Proteomics Reveals Molecular Trajectories Of Tangle-Bearing Neurons In Alzheimer’s Disease
Foiani MS, Bourdenx M, Kraller L, Nirujogi RS, Yiu A, Davies H, Patel S, Damoc LS, Mitchener L, Jaunmuktane Z, Coscia F, Duff KE
bioRxiv, 29 April 2026

Spatial Genomics (DNA-Reserach)

3D pathology-guided microdissection
Hsieh HC, Gao G, Han Q, Brenes D, Baraznenok E, Yan R, Serafin R, Bishop KW, Wang R, Konnick EQ, Pritchard CC, Figiel S, Hamdy FC, Mills IG, Reder NP, Reddi DM, Paulson TG, Grady WM, Valk JE, True LD, Haffner MC, Rao SR, Woodcock DJ, Liu JTC
Nat Methods (2026).

Geospatial genetic evolution and phenotypic plasticity in triple-negative breast cancer
Hong R, Wu P, Qiu Y, Dong S, Huang Y, Lee K, Wang H, Li M, Zhang C, Ye F, Mo S, Xue S, Wu W, Hu Z, Zhang W, Zhan Q, Li G, Wang S
Genome Med. 2026 Jun 10. doi: 10.1186/s13073-026-01684-0.

Clonal origin and early lineage divergence in adenosquamous carcinoma
Poulain F, Malassigné V, Roncarati P, Puech J, Imbeaud S, Zucman-Rossi J, Crum CP, Delvenne P, Hubert P, Péré H, Veyer D, Herfs M
Cell Rep. 2026 May 28;45(6):117470. doi: 10.1016/j.celrep.2026.117470.

Spatial Transcriptomics (RNA Research)

Integrative analysis of smart-seq2 and DIA proteomics reveal dynamic formation of the Balbiani body during oocyte development in leopard coral grouper (Plectropomus leopardus)
Liu J, Wang G, Zhang X, Wu X, Zhang X, Zheng T, Wen X, Wu X, Luo J
Research Square, 15th June 2026

Patient-matched transcriptomics of in vivo and in vitro colonic epithelium substantiate organoids as a translational model for ulcerative colitis
Walaas GAE, Sridhar A, Kuraas LF, Sæterstad S, Schioldborg AY, Van Beelen Granlund A, Sandvik AK, Østvik AE, Bakke I, Bruland T
Sci Rep. 2026 Jun 14. doi: 10.1038/s41598-026-54857-7.

Improved laser capture microdissection combined with transcriptome sequencing reveals gene expression reprogramming during rice panicle branch meristems differentiation
Yan G, Song T, Xu L, Li J, Wan B, Zhou L, Wu H, Zhang Y, Zhao J, Tong L, He L, Song Z, Zhang X, Wang W, Cai D, He Y
BMC Plant Biol. 2026 Jun 9. doi: 10.1186/s12870-026-08988-8.

Proteomics, Lipidomics, Metabolomics

Quantitative Global Proteomics of Formalin-Fixed Paraffin-Embedded Tumors Identifies Dimethylated G3BP1 at Arginine 460 as a Robust Pharmacodynamic Biomarker of Methylthioadenosine Phosphorylase-Selective Protein Arginine Methyltransferase 5 Inhibition
Tu A, Wiseman EJ, Sweet SM, Urosevic J, Loizou J, Barnicle A, Richardson C, Abhishek S, Chain DC, Kim YJ, Chambers AG
J Proteome Res. 2026 Jun 19. doi: 10.1021/acs.jproteome.6c00112.

Proteomics reveals spatial and molecular heterogeneities in advanced atherosclerotic carotid artery plaques
Sinha A, Sachs N, Kratz E, Pauli J, Steigenwald S, Albrecht V, Nordmann T, Ugur E, Rodriguez EH, Engl M-L, Skowronek P, Oliinyk D, Metousis A, von Scheidt M, Winter H, Schunkert H, Branzan D, Maegdefessel L, Mann M
Nat Cardiovasc Res (2026). 

Proteomic analyses reveal molecular changes underlying the preferential vulnerability of the human female CA1 hippocampal subfield across Braak stages in Alzheimer’s disease
Soriano-Herrador C, Ubeda-Banon I, Villanueva-Anguita P, Montesinos-Vinader JL, Saiz-Sanchez D, Astillero-Lopez V, Alberdi-Velez P, Fernandez-Irigoyen J, Lachen-Montes M, Santamaria E, Martinez-Marcos A, Flores-Cuadrado
Research Square, 10th June 2026

Spatial Single-Cell Proteomics Reveals Molecular Trajectories Of Tangle-Bearing Neurons In Alzheimer’s Disease
Foiani MS, Bourdenx M, Kraller L, Nirujogi RS, Yiu A, Davies H, Patel S, Damoc LS, Mitchener L, Jaunmuktane Z, Coscia F, Duff KE
bioRxiv, 29 April 2026

Deep Visual Proteomics (DVP) related

Spatial Visual Proteomics: Insights into Tumor Microenvironment Dynamics
Huang P, Fu C, Kong Q, Tian R
Genomics Proteomics Bioinformatics. 2026 Jun 16:qzag045. doi: 10.1093/gpbjnl/qzag045

Light-driven spatial proteomics: Photocatalytic strategies for mapping protein microenvironments
Somalwar N, Nan X, Emili A
Curr Opin Chem Biol. 2026 May 13;93:102696. doi: 10.1016/j.cbpa.2026.102696.

Evolution in single-cell proteomics drives new frontiers in cancer biology and precision medicine
Liu Y, Du Y, Su R, and Chen 
Cancer Biol Med 2026. doi: 10.20892/j.issn.2095-3941.2025.0464

Fluorescence Applications

A molecularly defined neural circuit for cold defense revealed by targeted single-nucleus transcriptomic mapping
Wu Y, Liu Z, Xiong T, Fan L, Chen Y, Duan X, Liu Y, Jing H, Liu X, Zhu Q, Zhang S, Liang W, Lu X, Ji SJ, Chen W, Zhu Y, Zhai J, Song K
Research Square, May 27th, 2026

Deep visual proteomics uncovers nociceptor diversity and pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
Nat Commun. 2026 Apr 11;17(1):3437. doi: 10.1038/s41467-026-71418-8.

Pathogenic Role of SERPINB3-positive Neutrophils in Reinforcing Thrombus Stiffening during Ischemic Stroke
Zang J, Lu A, Yang B, Tan N, Liu Q, Wei L, Liang Y, Zhou S, Tan Z, Xin X, He S, Wu P, Li Y, Xu Z, Su X, Mai H, Xu A, Lu D
Blood. 2026 Mar 3:blood.2025029516. doi: 10.1182/blood.2025029516.

Cancer Research

Quantitative Global Proteomics of Formalin-Fixed Paraffin-Embedded Tumors Identifies Dimethylated G3BP1 at Arginine 460 as a Robust Pharmacodynamic Biomarker of Methylthioadenosine Phosphorylase-Selective Protein Arginine Methyltransferase 5 Inhibition
Tu A, Wiseman EJ, Sweet SM, Urosevic J, Loizou J, Barnicle A, Richardson C, Abhishek S, Chain DC, Kim YJ, Chambers AG
J Proteome Res. 2026 Jun 19. doi: 10.1021/acs.jproteome.6c00112.

3D pathology-guided microdissection
Hsieh HC, Gao G, Han Q, Brenes D, Baraznenok E, Yan R, Serafin R, Bishop KW, Wang R, Konnick EQ, Pritchard CC, Figiel S, Hamdy FC, Mills IG, Reder NP, Reddi DM, Paulson TG, Grady WM, Valk JE, True LD, Haffner MC, Rao SR, Woodcock DJ, Liu JTC
Nat Methods (2026).

Geospatial genetic evolution and phenotypic plasticity in triple-negative breast cancer
Hong R, Wu P, Qiu Y, Dong S, Huang Y, Lee K, Wang H, Li M, Zhang C, Ye F, Mo S, Xue S, Wu W, Hu Z, Zhang W, Zhan Q, Li G, Wang S
Genome Med. 2026 Jun 10. doi: 10.1186/s13073-026-01684-0.

Neurosciences

Proteomic analyses reveal molecular changes underlying the preferential vulnerability of the human female CA1 hippocampal subfield across Braak stages in Alzheimer’s disease
Soriano-Herrador C, Ubeda-Banon I, Villanueva-Anguita P, Montesinos-Vinader JL, Saiz-Sanchez D, Astillero-Lopez V, Alberdi-Velez P, Fernandez-Irigoyen J, Lachen-Montes M, Santamaria E, Martinez-Marcos A, Flores-Cuadrado A
Research Square, 10th June 2026

POINTseq: Cell-type-specific barcoding reveals single-cell projection architecture of the mouse dopaminergic system
Kim H, Xu C, Washington C, Shi C, Lowman M, Kebschull JM
Neuron. 2026 Jun 4:S0896-6273(26)00376-4. doi: 10.1016/j.neuron.2026.05.005.

Spatiotemporal patterns of gene expression changes in the mouse dentate gyrus following entorhinal denervation
Schlaudraff J, Del Turco D, Key J, Deller T, Auburger G
Front. Mol. Neurosci., 19 May 2026, Sec. Neuroplasticity and Development, Volume 19 - 2026

Clinical/Pathology/Immunology Research Applications

Proteomics reveals spatial and molecular heterogeneities in advanced atherosclerotic carotid artery plaques
Sinha A, Sachs N, Kratz E, Pauli J, Steigenwald S, Albrecht V, Nordmann T, Ugur E, Rodriguez EH, Engl M-L, Skowronek P, Oliinyk D, Metousis A, von Scheidt M, Winter H, Schunkert H, Branzan D, Maegdefessel L, Mann M
Nat Cardiovasc Res (2026). 

Patient-matched transcriptomics of in vivo and in vitro colonic epithelium substantiate organoids as a translational model for ulcerative colitis
Walaas GAE, Sridhar A, Kuraas LF, Sæterstad S, Schioldborg AY, Van Beelen Granlund A, Sandvik AK, Østvik AE, Bakke I, Bruland T
Sci Rep. 2026 Jun 14. doi: 10.1038/s41598-026-54857-7.

Activation of α7nAChR Signaling Preserves Podocyte Structure and Suppresses Inflammatory Injury in Sepsis-Associated AKI
Wu HC, Yang, A, Shimoyama K, YamamotoR, Umene R, Nakamura Y, Zhang Y, Inoue T
Research Square, 25th May 2026

Developmental Research, Epigenetics & Aging

Integrative analysis of smart-seq2 and DIA proteomics reveal dynamic formation of the Balbiani body during oocyte development in leopard coral grouper (Plectropomus leopardus)
Liu J, Wang G, Zhang X, Wu X, Zhang X, Zheng T, Wen X, Wu X, Luo J
Research Square, 15th June 2026

CrAGL62 and potential paternally expressed gene CrbZIP contribute to paternal-excess postzygotic barriers in citrus
He JJ, Shen MY, Hu G, Xia QM, Duan YY, Xiao GA, Wu XM, Guo WW, Xie KD
New Phytol. 2026 Jun 17. doi: 10.1111/nph.71369.

Fetal inflammatory signals regulate maternal investment during marsupial pregnancy
Stadtmauer DJ, Maziarz JD, Griffith OW, Wagner GP
PLoS Biol. 2026 Feb 24;24(2):e3003670. doi: 10.1371/journal.pbio.3003670.

Plant Research

CrAGL62 and potential paternally expressed gene CrbZIP contribute to paternal-excess postzygotic barriers in citrus
He JJ, Shen MY, Hu G, Xia QM, Duan YY, Xiao GA, Wu XM, Guo WW, Xie KD
New Phytol. 2026 Jun 17. doi: 10.1111/nph.71369.

Improved laser capture microdissection combined with transcriptome sequencing reveals gene expression reprogramming during rice panicle branch meristems differentiation
Yan G, Song T, Xu L, Li J, Wan B, Zhou L, Wu H, Zhang Y, Zhao J, Tong L, He L, Song Z, Zhang X, Wang W, Cai D, He Y
BMC Plant Biol. 2026 Jun 9. doi: 10.1186/s12870-026-08988-8.

Extração de RNA total de células-alvo  isoladas por microdissecção a laser
Schietti R, Faria DA, Aguiar NN, Araujo ACG
Embrapa Recursos Genéticos e Biotecnologia. Circular Técnica, 100

Forensics

Separation and identification of two-individual mixed bloodstains using human neutrophil antigen-1 antibodies
Xia X, Zheng JL, Wang SW, Liu Z, Li Z, Wang DY, Li XN, Wu X, Yao J
Int J Legal Med. 2026 Jan 27. doi: 10.1007/s00414-026-03718-z.

Invisible WItness: Innovations in the Collection of Touch DNA and the Risk of Forensic Corss Contamination
Irfan M, Haider SA, Yasir M, Akram MA, Adeel M
FORENSIC INSIGHTS AND HEALTH SCIENCES BULLETIN, Volume 3: Issue 2: 2025

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Miscellaneous

Spatial Visual Proteomics: Insights into Tumor Microenvironment Dynamics
Huang P, Fu C, Kong Q, Tian R
Genomics Proteomics Bioinformatics. 2026 Jun 16:qzag045. doi: 10.1093/gpbjnl/qzag045

Development and Validation of a HAUS-Related Prognostic Signature in Liver Hepatocellular Carcinoma: A Multi-Omics Integration from PanCancer Analysis to Single-Cell Resolution
Liu Y, Liu K, Ding Y, Li X, Shi X, Zhang J, Zhang X, Peng W, Deng J & Chen J
Journal of Hepatocellular Carcinoma, 598179, DOI: 10.2147/JHC.S598179

Cryptosporidium secreted proteins form a complex layered interface with the host cell
Puthenpurackal A, Sanchez SM, Schaer T, Falconnet L, Niu C, Vadas O, Guérin A
PLoS Pathog. 2026 May 14;22(5):e1014212. doi: 10.1371/journal.ppat.1014212.

Live Cell Culture

Cyanobacterial extracellular polysaccharides (EPS) produced at high CO2 enhance sequestration of heavy metals into mineral precipitate
Rajapitamahuni S, Khilyas IV, Kusibu MM, Cohen MF, Kamennaya NA
Sci Rep. 2026 May 14. doi: 10.1038/s41598-026-53196-x.

Deep visual proteomics uncovers nociceptor diversity and pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
Nat Commun. 2026 Apr 11;17(1):3437. doi: 10.1038/s41467-026-71418-8.

Muscle Structure and Function Recovery: Adalimumab-Calcium Channel Synergy in Post-Ischemic Stroke Sarcopenia
Qi H, Zhang XW, Zhang ZY, Ou SS, Gao YL, Tian D, Jiang YN, Min XR, Zhao AT, Zou JM, Zeng JS, Pu QY, Yang RC, Zeng N
J Cachexia Sarcopenia Muscle. 2025 Dec;16(6):e70097. doi: 10.1002/jcsm.70097.

Ultrasensitive proteomics uncovers nociceptor diversity and novel pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
bioRxiv, Sept 21, 2025

Laser microdissection (LMD) is an advanced, highly precise sample preparation technique that enables researchers to isolate pure, defined regions of interest, including single cells, from complex tissue samples. The method ensures reproducibility and accuracy for downstream analyses such as Next Generation Sequencing (NGS) including RNA-seq, and mass spectrometry for spatial genomics, spatial transcriptomics, spatial proteomics & spatial multiomics.

LMD allows non-contact dissection, isolation and gravity-assisted collection of targeted single cells, tissue compartments, cell clusters and even subcellular structures under visual control. These systems uniquely bridge microscopy and molecular biology to unlock true spatial omics insights. Combined with software-guided segmentation, Leica LMD systems enable high throughput workflows for biomarker discovery and identification of therapeutical interaction points. The study by Mund & Coscia et al., describing the Deep Visual Proteomics (DVP) workflow powered by the Leica LMD7 system, was featured on the cover of Nature Biotechnology in August 2022.  Follow-up research by Nordmann et al., published in Nature in 2024, demonstrated how DVP contributed to a life-saving therapy for toxic epidermal necrolysis (TEN), a deadly skin disease. These breakthroughs also contributed to the selection of “spatial proteomics” as Nature’s Method of the Year 2024.