Leica LMD6 & LMD7 Laser Microdissection Microscopes

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

SERBP1-PCIF1 complex-controlled m6Am modification in glutamatergic neurons of the primary somatosensory cortex is required for neuropathic pain in mice
Huang Y, Ma G, Xie S, Wei R, Liu Y, Zeng Y, Zhao Y, Wang Q, Yang L, Huang H, Hao L, Zhao X, Wang H, Shen W, Wong SSC, Cao JL, Tao YX, Pan ZQ
Nat Commun. 2025 Aug 5;16(1):7225. doi: 10.1038/s41467-025-62565-5.

Frontal cortex pyramidal neuron expression profiles differentiate the prodromal stage from progressive degeneration across the Alzheimer's disease spectrum
Labuza A, Alldred MJ, Pidikiti H, Malek-Ahmadi MH, Lee SH, Heguy A, Coleman PD, Chakrabarty S, Chiosis G, Mufson EJ, Ginsberg SD
Alzheimers Dement. 2025 Jul;21(7):e70395. doi: 10.1002/alz.70395.

Maternal smoking in early pregnancy disrupts placental function through syncytiotrophoblast and macrophage dysregulation
Valdes DS, Nimo J, Nonn, O, Ulrich J, Forstner D, Haider S, Ressler M, Pignitter M, Muller DN, Dechend R, Gauster M, Coscia F, Herse F
bioRxiv, July 15, 2025.

DNA Research

A novel m.14677 T > C variant in mitochondrial tRNAGlu gene causes chronic progressive external ophthalmoplegia
Ueda NK, Mimaki M, Ito S, Murakami A, Yokoi S, Nishino I, Katsuno M, Goto YI
J Hum Genet. 2025 Aug 6. doi: 10.1038/s10038-025-01381-7.

Origin of Endometrioid Carcinoma Adjacent to Extraovarian Endometriosis: Detailed Molecular Analyses.
Yamagata T, Watanabe K, Yamanoi K, Kakiuchi N, Mizuno R, Taki M, Murakami R, Yamaguchi K, Hamanishi J, Itoh H, Ogawa S, Mandai M
Int J Gynecol Pathol. 2025 Jul 29. doi: 10.1097/PGP.0000000000001128.

Clinicopathologic and Genomic Features of Gastric-Type Intraductal Papillary Neoplasm of the Bile Duct: Potential Role of STK11 in Malignant Progression
Shimada Y, Yamamoto T, Shindo K, Nakanishi Y, Matsumoto T, Noguchi S, Aishima S, Nakamura M, Oda Y
Am J Surg Pathol. 2025 Jul 11. doi: 10.1097/PAS.0000000000002451.

RNA Research

A cell isolation method from Ligusticum chuanxiong Hort. suitable for obtaining high-quality RNA for Smart-seq
Li R, Wu M, Chen S, Huang L, Wang C, Yu Z, Huang F, Liu X, Zhu N, Song C, Jiang G, Yin X
Plant Methods. 2025 Aug 10;21(1):109. doi: 10.1186/s13007-025-01425-1.

Effects of proximal tubule-specific ATRAP enhancement on hypertension in a remnant kidney chronic kidney disease model of mice
Okami N, Wakui H, Tanaka S, Kobayashi R, Tsukamoto S, Taguchi S, Morita R, Kubo E, Yamashita A, Azushima K, Tamura K
Sci Rep. 2025 Jul 28;15(1):27391. doi: 10.1038/s41598-025-12168-3.

Single-cell and spatial multiomics identifies heterogeneous xylem development driven by mechanical stress in Populus
Hsieh JA, Liou PC, Lin CC, Dai X, Hu CW, Wang IF, Dang JF, Ho YC, Cheng KW, Xu W, Kuo SC, Kao CT, Yang DX, Wang R, Xiao K, Lin JS, Hsu CC, Hsu CC, Mai TL, Cheng Y, Tseng MC, Li W, Chen YL, Lin YJ
Dev Cell. 2025 Jul 21:S1534-5807(25)00436-8. doi: 10.1016/j.devcel.2025.06.038.

Proteomics, Lipidomics, Metabolomics

A new paradigm of islet adaptations in human pregnancy: insights from immunohistochemistry and proteomics.
Seedat F, Holden K, Davis S, Fischer R, Bancroft J, Drydale E, Kandzija N, Todd JA, Vatish M, Stefana MI
Nat Commun. 2025 Jul 21;16(1):6687. doi: 10.1038/s41467-025-61852-5.

Development of a novel targeted LC-MS/MS Methods for the typing of cardiac amyloidosis
Wang K, Liu R, Li L, Duan X, Zhao X, Cong H, Gao X, Yin K, Gao Z, Zhou Z
Analytica Chimica Acta, 18 July 2025, 344453

A perspective on integrating digital pathology, proteomics, clinical data and AI analytics in cancer research.
Guedes J, Woldmar N, Szasz AM, Wieslander E, Pawłowski K, Horvatovich P, Malm J, Szadai L, Németh IB, Marko-Varga G, Gil J
J Proteomics. 2025 Jul 16;320:105493. doi: 10.1016/j.jprot.2025.105493.

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Highlight 2025: 

Rosenberger FA, et al.
Nature (2025 Apr 16)
https://www.nature.com/articles/s41586-025-08885-4

Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2

Highlights 2024: 

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

SERBP1-PCIF1 complex-controlled m6Am modification in glutamatergic neurons of the primary somatosensory cortex is required for neuropathic pain in mice
Huang Y, Ma G, Xie S, Wei R, Liu Y, Zeng Y, Zhao Y, Wang Q, Yang L, Huang H, Hao L, Zhao X, Wang H, Shen W, Wong SSC, Cao JL, Tao YX, Pan ZQ
Nat Commun. 2025 Aug 5;16(1):7225. doi: 10.1038/s41467-025-62565-5.

Assessing blood-brain barrier (BBB) integrity in an Alzheimer's disease mouse model: is the BBB globally or locally disrupted?
Nozohouri E, Noorani B, Patel D, Ahn Y, Zoubi S, Bickel U
Fluids Barriers CNS. 2025 Jul 23;22(1):79. doi: 10.1186/s12987-025-00685-2.

A new paradigm of islet adaptations in human pregnancy: insights from immunohistochemistry and proteomics.
Seedat F, Holden K, Davis S, Fischer R, Bancroft J, Drydale E, Kandzija N, Todd JA, Vatish M, Stefana MI
Nat Commun. 2025 Jul 21;16(1):6687. doi: 10.1038/s41467-025-61852-5.

Cancer Research

Proteogenomic characterization of invasive breast tumors in young women
Raj-Kumar PK, Liu J, Soltis AR, Bateman NW, Chen Q, Sturtz LA, Deyarmin B, Pierobon M, Abulez TA, Praveen-Kumar A, Zhang X, Nguyen T, Yan C, Hu Y, Guion K, Hooke JA, Kovatich AJ, Fantacone-Campbell L, Mostoller B, Kvecher L, Somiari S, Steeg PS, Rajagopal PS, Darcy KM, Lee JSH, Dalgard CL, Conrads TP, Petricoin EF, Meerzaman D, Wilkerson MD; APOLLO Research Network; Lin X, Shriver CD, Lipkowitz S, Hu H
NPJ Breast Cancer. 2025 Aug 18;11(1):94. doi: 10.1038/s41523-025-00793-0.

Origin of Endometrioid Carcinoma Adjacent to Extraovarian Endometriosis: Detailed Molecular Analyses.
Yamagata T, Watanabe K, Yamanoi K, Kakiuchi N, Mizuno R, Taki M, Murakami R, Yamaguchi K, Hamanishi J, Itoh H, Ogawa S, Mandai M
Int J Gynecol Pathol. 2025 Jul 29. doi: 10.1097/PGP.0000000000001128.

Genomic characterization of malignant progression in neoplastic pancreatic cysts
Noë M, Niknafs N, Fischer CG, Hackeng WM, Beleva Guthrie V, Hosoda W, Debeljak M, Papp E, Adleff V, White JR, Luchini C, Pea A, Scarpa A, Butturini G, Zamboni G, Castelli P, Hong SM, Yachida S, Hiraoka N, Gill AJ, Samra JS, Offerhaus GJA, Hoorens A, Verheij J, Jansen C, Adsay NV, Jiang W, Winter J, Albores-Saavedra J, Terris B, Thompson ED, Roberts NJ, Hruban RH, Karchin R, Scharpf RB, Brosens LAA, Velculescu VE, Wood LD
Nat Commun. 2020 Aug 14;11(1):4085. doi: 10.1038/s41467-020-17917-8.

Neurosciences

Frontal cortex pyramidal neuron expression profiles differentiate the prodromal stage from progressive degeneration across the Alzheimer's disease spectrum
Labuza A, Alldred MJ, Pidikiti H, Malek-Ahmadi MH, Lee SH, Heguy A, Coleman PD, Chakrabarty S, Chiosis G, Mufson EJ, Ginsberg SD
Alzheimers Dement. 2025 Jul;21(7):e70395. doi: 10.1002/alz.70395.

Proteomics of the temporal cortex in semantic dementia reveals brain-region specific molecular pathology and regulation of the TDP-43-ANXA11 interactome
Miedema SSM, Rajicic A, Mol MO, Paliukhovich I, Klaassen RV, van Buuren R, Li KW, Koopmans FTW, Seelaar H, van Rooij JGJ, Smit AB, van Swieten JC
Acta Neuropathol Commun. 2025 Jul 25;13(1):162. doi: 10.1186/s40478-025-02077-x.

Assessing blood-brain barrier (BBB) integrity in an Alzheimer's disease mouse model: is the BBB globally or locally disrupted?
Nozohouri E, Noorani B, Patel D, Ahn Y, Zoubi S, Bickel U
Fluids Barriers CNS. 2025 Jul 23;22(1):79. doi: 10.1186/s12987-025-00685-2.

Clinical/Pathology/Immunology Research Applications

Impact of PEGylation and hyaluronan functionalization on lipoplex-mediated mRNA delivery to the canine retina
Appelbaum T, Smith DA, Takahashi K, Kwok JC, Sorenson H, Beltran WA
Drug Deliv. 2025 Dec;32(1):2544688. doi: 10.1080/10717544.2025.2544688.

A novel m.14677 T > C variant in mitochondrial tRNAGlu gene causes chronic progressive external ophthalmoplegia
Ueda NK, Mimaki M, Ito S, Murakami A, Yokoi S, Nishino I, Katsuno M, Goto YI
J Hum Genet. 2025 Aug 6. doi: 10.1038/s10038-025-01381-7.

Effects of proximal tubule-specific ATRAP enhancement on hypertension in a remnant kidney chronic kidney disease model of mice
Okami N, Wakui H, Tanaka S, Kobayashi R, Tsukamoto S, Taguchi S, Morita R, Kubo E, Yamashita A, Azushima K, Tamura K
Sci Rep. 2025 Jul 28;15(1):27391. doi: 10.1038/s41598-025-12168-3.

Parenchymal and Dyshoric Fibrillar Amyloid Pathology in the rTg-D Rat Model of Cerebral Amyloid Angiopathy Type-2
Schrader JM, Xu F, Zhu X, Majchrzak M, Davis J, Van Nostrand WE
Am J Pathol. 2025 Jul 17:S0002-9440(25)00241-X. doi: 10.1016/j.ajpath.2025.06.008.

Developmental Research, Epigenetics & Aging

Single-cell and spatial multiomics identifies heterogeneous xylem development driven by mechanical stress in Populus
Hsieh JA, Liou PC, Lin CC, Dai X, Hu CW, Wang IF, Dang JF, Ho YC, Cheng KW, Xu W, Kuo SC, Kao CT, Yang DX, Wang R, Xiao K, Lin JS, Hsu CC, Hsu CC, Mai TL, Cheng Y, Tseng MC, Li W, Chen YL, Lin YJ
Dev Cell. 2025 Jul 21:S1534-5807(25)00436-8. doi: 10.1016/j.devcel.2025.06.038.

A new paradigm of islet adaptations in human pregnancy: insights from immunohistochemistry and proteomics.
Seedat F, Holden K, Davis S, Fischer R, Bancroft J, Drydale E, Kandzija N, Todd JA, Vatish M, Stefana MI
Nat Commun. 2025 Jul 21;16(1):6687. doi: 10.1038/s41467-025-61852-5.

Maternal smoking in early pregnancy disrupts placental function through syncytiotrophoblast and macrophage dysregulation
Valdes DS, Nimo J, Nonn, O, Ulrich J, Forstner D, Haider S, Ressler M, Pignitter M, Muller DN, Dechend R, Gauster M, Coscia F, Herse F
bioRxiv, July 15, 2025.

Plant Research

A cell isolation method from Ligusticum chuanxiong Hort. suitable for obtaining high-quality RNA for Smart-seq
Li R, Wu M, Chen S, Huang L, Wang C, Yu Z, Huang F, Liu X, Zhu N, Song C, Jiang G, Yin X
Plant Methods. 2025 Aug 10;21(1):109. doi: 10.1186/s13007-025-01425-1.

Plant defense-directed discovery of a natural anti-psoriasis agent targeting GAPDH
Zhou TT, Zheng Y, Zhang MW, Gong LH, Guo K, He XP, Liu YC, Gershenzon J, Liu Y, Li SH
Sci Adv. 2025 Jul 25;11(30):eadw2578. doi: 10.1126/sciadv.adw2578.

Comprehensive analysis of imprinted genes in citrus endosperm and their contributions to seed development
He JJ, Hu G, Shen MY, Fan YJ, Shi XS, Wu XM, Guo WW, Xia QM, Xie KD
Plant J. 2025 Jun;122(6):e70290. doi: 10.1111/tpj.70290.

Single-cell and spatial multiomics identifies heterogeneous xylem development driven by mechanical stress in Populus
Hsieh JA, Liou PC, Lin CC, Dai X, Hu CW, Wang IF, Dang JF, Ho YC, Cheng KW, Xu W, Kuo SC, Kao CT, Yang DX, Wang R, Xiao K, Lin JS, Hsu CC, Hsu CC, Mai TL, Cheng Y, Tseng MC, Li W, Chen YL, Lin YJ
Dev Cell. 2025 Jul 21:S1534-5807(25)00436-8. doi: 10.1016/j.devcel.2025.06.038.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Functional food prevents diabetic nephropathy in a rat model through inhibition of aldose reductase and accumulation of advanced glycation end-products
Kalahasti KK, Nagaraju M, Jakhotia S, Reddy SS, Reddy GB
PharmaNutrition, Volume 33, September 2025, 100446

In Situ Metabolic Rates of Alkane-Degrading Sulphate-Reducing Bacteria in Hydrocarbon Seep Sediments Revealed by Combining CARD-FISH, NanoSIMS, and Mathematical Modelling
Kleindienst S, Polerecky L, Amann R, Musat F, Knittel K
Environ Microbiol. 2025 Aug;27(8):e70151. doi: 10.1111/1462-2920.70151.

Morphological Control of Microbial Ecosystems and Carbon Cycling in Greenlandic Cryoconite Holes
Takeuchi N, Takahiro S, Murakami T, Ishiwatari K, Watanabe A
Research Square

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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416