Leica LMD6 & LMD7 Laser Microdissection Microscopes

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Integration of cell-type resolved spatial proteomics and transcriptomics reveals novel mechanisms in early ovarian cancer
Metousis A, Kenny HA, Shimizu A, Schweizer L. Ben-Moshe S, Bilecz A, Krishnan R, Zhang J, Alcazar I, Kelliher L, Ravi M, Samantaray T, Richter S, Li Y, Wang J, Steigerwald S, Theis FJ, Rosenberger FA, Nordmann TM, Yamada SD, Lastra R, Mann M, and Lengyel E
medRxiv, 28 Aug 2025

ALS/FTD-linked TBK1 deficiency in microglia induces an aged-like microglial signature and drives social recognition deficits in mice
Lenoel I, Ribon M, Lorenc F, Diebold A, Philibert CE, Robaldo D, Badsi M, Perronnet J, Lameth J, Berriat F, Misawa H, Coutelier M, Cassel R, Sarrazin N, Jost-Mousseau C, Bohl D, Millecamps S, Mallat M, Brenner D, Weishaupt JH, Boillée S, Lobsiger CS
Nat Commun. 2025 Aug 26;16(1):7951. doi: 10.1038/s41467-025-63211-w.

Single-cell multi-omics in cancer immunotherapy: from tumor heterogeneity to personalized precision treatment
Le J, Dian Y, Zhao D, Guo Z, Luo Z, Chen X, Zeng F, Deng G
Mol Cancer. 2025 Aug 25;24(1):221. doi: 10.1186/s12943-025-02426-3.

DNA Research

A novel m.14677 T > C variant in mitochondrial tRNAGlu gene causes chronic progressive external ophthalmoplegia
Ueda NK, Mimaki M, Ito S, Murakami A, Yokoi S, Nishino I, Katsuno M, Goto YI
J Hum Genet. 2025 Aug 6. doi: 10.1038/s10038-025-01381-7.

Origin of Endometrioid Carcinoma Adjacent to Extraovarian Endometriosis: Detailed Molecular Analyses.
Yamagata T, Watanabe K, Yamanoi K, Kakiuchi N, Mizuno R, Taki M, Murakami R, Yamaguchi K, Hamanishi J, Itoh H, Ogawa S, Mandai M
Int J Gynecol Pathol. 2025 Jul 29. doi: 10.1097/PGP.0000000000001128.

Clinicopathologic and Genomic Features of Gastric-Type Intraductal Papillary Neoplasm of the Bile Duct: Potential Role of STK11 in Malignant Progression
Shimada Y, Yamamoto T, Shindo K, Nakanishi Y, Matsumoto T, Noguchi S, Aishima S, Nakamura M, Oda Y
Am J Surg Pathol. 2025 Jul 11. doi: 10.1097/PAS.0000000000002451.

RNA Research

Temporal transcriptional regulation of mitochondrial morphology primes activity-dependent circuit connectivity
Mohylyak I, Andriatsilavo M, Bengochea M, Pascual-Caro C, Asfogo N, Fonseca-Topp S, Danda N, Cassar M, Marie C, Atak ZK, De Waegeneer M, Aerts S, Corti O, de Juan-Sanz J, Hassan BA
Nat Commun. 2025 Sep 1;16(1):8173. doi: 10.1038/s41467-025-62908-2.

Adipose tissue macrophage-derived PPBP exacerbates psoriasis-associated atherosclerosis by inducing mitochondrial dysfunction in aortic endothelial cells
Zhu L, Du L, Wu J, Ding D, Wang J, Wang R, Liu Z, Kuzmina N, Wang H, Yang Y, Ståhle M, Landén NX, Li S, Li D
J Invest Dermatol. 2025 Aug 29:S0022-202X(25)02396-6. doi: 10.1016/j.jid.2025.05.041.

ALS/FTD-linked TBK1 deficiency in microglia induces an aged-like microglial signature and drives social recognition deficits in mice
Lenoel I, Ribon M, Lorenc F, Diebold A, Philibert CE, Robaldo D, Badsi M, Perronnet J, Lameth J, Berriat F, Misawa H, Coutelier M, Cassel R, Sarrazin N, Jost-Mousseau C, Bohl D, Millecamps S, Mallat M, Brenner D, Weishaupt JH, Boillée S, Lobsiger CS
Nat Commun. 2025 Aug 26;16(1):7951. doi: 10.1038/s41467-025-63211-w.

Proteomics, Lipidomics, Metabolomics

Spatially Resolved Lipid Composition of the Human Brain Cortical Layers
Senko D, Zavolskova M, Efimova O, Osetrova M, Stekolshchikova E, Vladimirov G, Nikolaev E, Khaitovich P
Neurosci Bull. 2025 Aug 29. doi: 10.1007/s12264-025-01486-1.

Multi-omic profiling of intraductal papillary neoplasms of the pancreas reveals distinct patterns and potential markers of progression
Wang Y, Lih TM, Lee JW, Ohtsuka T, Hozaka Y, Mino-Kenudson M, Adsay NV, Luchini C, Scarpa A, Maker AV, Kim GE, Paulino J, Chen L, Woo J, Jiao L, Sun Z, Goodman D, Pflüger MJ, Roberts NJ, Matthaei H, Goggins MG, Wood LD, Furukawa T, Zhang H, Hruban RH
Cancer Cell. 2025 Aug 26:S1535-6108(25)00332-0. doi: 10.1016/j.ccell.2025.08.001.

Atypical mesenchyme in congenital pulmonary airways malformation: a promising new focus
Azar P, Avila Y, Hiltbrunner A, Delacourt C, Rougemont AL, Vidal I, Bochaton-Piallat ML, Ruchonnet-Metrailler I
Respir Res. 2025 Aug 26;26(1):262. doi: 10.1186/s12931-025-03332-4.

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Highlight 2025: 

Rosenberger FA, et al.
Nature (2025 Apr 16)
https://www.nature.com/articles/s41586-025-08885-4

Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2

Highlights 2024: 

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Deep Visual Proteomics (DVP) related

Integration of cell-type resolved spatial proteomics and transcriptomics reveals novel mechanisms in early ovarian cancer
Metousis A, Kenny HA, Shimizu A, Schweizer L. Ben-Moshe S, Bilecz A, Krishnan R, Zhang J, Alcazar I, Kelliher L, Ravi M, Samantaray T, Richter S, Li Y, Wang J, Steigerwald S, Theis FJ, Rosenberger FA, Nordmann TM, Yamada SD, Lastra R, Mann M, and Lengyel E
medRxiv, 28 Aug 2025

Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Kenny HA, Krishnan R, Kelliher L, Bilecz AJ, Heide J, Donle L, Shimizu A, Metousis A, Mendoza R, Nordmann TM, Rauch S, Richter S, Yan L, Rosenberger FA, Strauss MT, Kurnit KC, Thielert M, Rodriguez E, Müller-Reif JB, Yamada SD, Theis FJ, Mund A, Lastra RR, Mann M, and Lengyei E
Cancer Cell, 2025 Aug 11;43(8):1495-1511.e7. doi: 10.1016/j.ccell.2025.06.004.

AI-powered Deep Visual Proteomics reveals critical molecular transitions in pancreatic cancer precursors
Min J, Schweizer L, Zonderland G, Selvanesan BC, Oldenburg L, Bae S-W, Kim BJ, Swanson BJ, Klute KA, Caffrey TC, Grandgenett PM, Hollingsworth MA, Ummat I, Strauss MT, Mund A, Maitra A
bioRxiv, 7 Jul 2025

Fluorescence Applications

Temporal transcriptional regulation of mitochondrial morphology primes activity-dependent circuit connectivity
Mohylyak I, Andriatsilavo M, Bengochea M, Pascual-Caro C, Asfogo N, Fonseca-Topp S, Danda N, Cassar M, Marie C, Atak ZK, De Waegeneer M, Aerts S, Corti O, de Juan-Sanz J, Hassan BA
Nat Commun. 2025 Sep 1;16(1):8173. doi: 10.1038/s41467-025-62908-2.

SERBP1-PCIF1 complex-controlled m6Am modification in glutamatergic neurons of the primary somatosensory cortex is required for neuropathic pain in mice
Huang Y, Ma G, Xie S, Wei R, Liu Y, Zeng Y, Zhao Y, Wang Q, Yang L, Huang H, Hao L, Zhao X, Wang H, Shen W, Wong SSC, Cao JL, Tao YX, Pan ZQ
Nat Commun. 2025 Aug 5;16(1):7225. doi: 10.1038/s41467-025-62565-5.

A new paradigm of islet adaptations in human pregnancy: insights from immunohistochemistry and proteomics.
Seedat F, Holden K, Davis S, Fischer R, Bancroft J, Drydale E, Kandzija N, Todd JA, Vatish M, Stefana MI
Nat Commun. 2025 Jul 21;16(1):6687. doi: 10.1038/s41467-025-61852-5.

Cancer Research

Integration of cell-type resolved spatial proteomics and transcriptomics reveals novel mechanisms in early ovarian cancer
Metousis A, Kenny HA, Shimizu A, Schweizer L. Ben-Moshe S, Bilecz A, Krishnan R, Zhang J, Alcazar I, Kelliher L, Ravi M, Samantaray T, Richter S, Li Y, Wang J, Steigerwald S, Theis FJ, Rosenberger FA, Nordmann TM, Yamada SD, Lastra R, Mann M, and Lengyel E
medRxiv, 28 Aug 2025

Multi-omic profiling of intraductal papillary neoplasms of the pancreas reveals distinct patterns and potential markers of progression
Wang Y, Lih TM, Lee JW, Ohtsuka T, Hozaka Y, Mino-Kenudson M, Adsay NV, Luchini C, Scarpa A, Maker AV, Kim GE, Paulino J, Chen L, Woo J, Jiao L, Sun Z, Goodman D, Pflüger MJ, Roberts NJ, Matthaei H, Goggins MG, Wood LD, Furukawa T, Zhang H, Hruban RH
Cancer Cell. 2025 Aug 26:S1535-6108(25)00332-0. doi: 10.1016/j.ccell.2025.08.001.

Proteogenomic characterization of invasive breast tumors in young women
Raj-Kumar PK, Liu J, Soltis AR, Bateman NW, Chen Q, Sturtz LA, Deyarmin B, Pierobon M, Abulez TA, Praveen-Kumar A, Zhang X, Nguyen T, Yan C, Hu Y, Guion K, Hooke JA, Kovatich AJ, Fantacone-Campbell L, Mostoller B, Kvecher L, Somiari S, Steeg PS, Rajagopal PS, Darcy KM, Lee JSH, Dalgard CL, Conrads TP, Petricoin EF, Meerzaman D, Wilkerson MD; APOLLO Research Network; Lin X, Shriver CD, Lipkowitz S, Hu H
NPJ Breast Cancer. 2025 Aug 18;11(1):94. doi: 10.1038/s41523-025-00793-0.

Neurosciences

Temporal transcriptional regulation of mitochondrial morphology primes activity-dependent circuit connectivity
Mohylyak I, Andriatsilavo M, Bengochea M, Pascual-Caro C, Asfogo N, Fonseca-Topp S, Danda N, Cassar M, Marie C, Atak ZK, De Waegeneer M, Aerts S, Corti O, de Juan-Sanz J, Hassan BA
Nat Commun. 2025 Sep 1;16(1):8173. doi: 10.1038/s41467-025-62908-2.

Spatially Resolved Lipid Composition of the Human Brain Cortical Layers
Senko D, Zavolskova M, Efimova O, Osetrova M, Stekolshchikova E, Vladimirov G, Nikolaev E, Khaitovich P
Neurosci Bull. 2025 Aug 29. doi: 10.1007/s12264-025-01486-1.

ALS/FTD-linked TBK1 deficiency in microglia induces an aged-like microglial signature and drives social recognition deficits in mice
Lenoel I, Ribon M, Lorenc F, Diebold A, Philibert CE, Robaldo D, Badsi M, Perronnet J, Lameth J, Berriat F, Misawa H, Coutelier M, Cassel R, Sarrazin N, Jost-Mousseau C, Bohl D, Millecamps S, Mallat M, Brenner D, Weishaupt JH, Boillée S, Lobsiger CS
Nat Commun. 2025 Aug 26;16(1):7951. doi: 10.1038/s41467-025-63211-w.

Clinical/Pathology/Immunology Research Applications

Uncovering molecular markers of the microvascular endothelial response in sepsis-associated acute kidney injury: a translational study in mice and humans
van der Aart TJ, Molema G, Jongman RM, Bouma HR, Koeze J, Moser J, van Londen M, Hackl M, Diendorfer AB, Ter Maaten JC, van Meurs M, Luxen M
Intensive Care Med Exp. 2025 Sep 2;13(1):92. doi: 10.1186/s40635-025-00801-4.

Adipose tissue macrophage-derived PPBP exacerbates psoriasis-associated atherosclerosis by inducing mitochondrial dysfunction in aortic endothelial cells
Zhu L, Du L, Wu J, Ding D, Wang J, Wang R, Liu Z, Kuzmina N, Wang H, Yang Y, Ståhle M, Landén NX, Li S, Li D
J Invest Dermatol. 2025 Aug 29:S0022-202X(25)02396-6. doi: 10.1016/j.jid.2025.05.041.

Gallbladder amyloidosis is often unexpected and may have systemic implications
Hagen CE, Dasari S, Theis JD, Rech K, Dao L, Howard M, Larson DP, Nasr SH, Dispenzieri A, Chiu A, Dalland J, Gertz M, Kourelis T, Muchtar E, Vrana JA, McPhail ED
Am J Clin Pathol. 2025 Aug 29:aqaf090. doi: 10.1093/ajcp/aqaf090.

Atypical mesenchyme in congenital pulmonary airways malformation: a promising new focus
Azar P, Avila Y, Hiltbrunner A, Delacourt C, Rougemont AL, Vidal I, Bochaton-Piallat ML, Ruchonnet-Metrailler I
Respir Res. 2025 Aug 26;26(1):262. doi: 10.1186/s12931-025-03332-4.
 

Developmental Research, Epigenetics & Aging

Single-cell and spatial multiomics identifies heterogeneous xylem development driven by mechanical stress in Populus
Hsieh JA, Liou PC, Lin CC, Dai X, Hu CW, Wang IF, Dang JF, Ho YC, Cheng KW, Xu W, Kuo SC, Kao CT, Yang DX, Wang R, Xiao K, Lin JS, Hsu CC, Hsu CC, Mai TL, Cheng Y, Tseng MC, Li W, Chen YL, Lin YJ
Dev Cell. 2025 Jul 21:S1534-5807(25)00436-8. doi: 10.1016/j.devcel.2025.06.038.

A new paradigm of islet adaptations in human pregnancy: insights from immunohistochemistry and proteomics.
Seedat F, Holden K, Davis S, Fischer R, Bancroft J, Drydale E, Kandzija N, Todd JA, Vatish M, Stefana MI
Nat Commun. 2025 Jul 21;16(1):6687. doi: 10.1038/s41467-025-61852-5.

Maternal smoking in early pregnancy disrupts placental function through syncytiotrophoblast and macrophage dysregulation
Valdes DS, Nimo J, Nonn, O, Ulrich J, Forstner D, Haider S, Ressler M, Pignitter M, Muller DN, Dechend R, Gauster M, Coscia F, Herse F
bioRxiv, July 15, 2025.

Plant Research

A cell isolation method from Ligusticum chuanxiong Hort. suitable for obtaining high-quality RNA for Smart-seq
Li R, Wu M, Chen S, Huang L, Wang C, Yu Z, Huang F, Liu X, Zhu N, Song C, Jiang G, Yin X
Plant Methods. 2025 Aug 10;21(1):109. doi: 10.1186/s13007-025-01425-1.

Plant defense-directed discovery of a natural anti-psoriasis agent targeting GAPDH
Zhou TT, Zheng Y, Zhang MW, Gong LH, Guo K, He XP, Liu YC, Gershenzon J, Liu Y, Li SH
Sci Adv. 2025 Jul 25;11(30):eadw2578. doi: 10.1126/sciadv.adw2578.

Comprehensive analysis of imprinted genes in citrus endosperm and their contributions to seed development
He JJ, Hu G, Shen MY, Fan YJ, Shi XS, Wu XM, Guo WW, Xia QM, Xie KD
Plant J. 2025 Jun;122(6):e70290. doi: 10.1111/tpj.70290.

Single-cell and spatial multiomics identifies heterogeneous xylem development driven by mechanical stress in Populus
Hsieh JA, Liou PC, Lin CC, Dai X, Hu CW, Wang IF, Dang JF, Ho YC, Cheng KW, Xu W, Kuo SC, Kao CT, Yang DX, Wang R, Xiao K, Lin JS, Hsu CC, Hsu CC, Mai TL, Cheng Y, Tseng MC, Li W, Chen YL, Lin YJ
Dev Cell. 2025 Jul 21:S1534-5807(25)00436-8. doi: 10.1016/j.devcel.2025.06.038.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Spatial proteomics in the age of artificial intelligence: exploring the impact on cancer research
Hagan J
BioTechniques, 1–5. 2025

Single-cell multi-omics in cancer immunotherapy: from tumor heterogeneity to personalized precision treatment
Le J, Dian Y, Zhao D, Guo Z, Luo Z, Chen X, Zeng F, Deng G
Mol Cancer. 2025 Aug 25;24(1):221. doi: 10.1186/s12943-025-02426-3.

One Shock, Not One Cure: Electroporation Reveals Disease-Specific Constraints in Hepatocyte Gene Editing Therapy
Clark C, Pokhrel M, Arthur B, Suresh P, Ates I, Gibson J, Dhungana A, Mehlem R, Boysia A, Padalkar MV, Pokhrel A, Echesabal-Chen J, Vonada A, Stamatikos A, Savinova OV, Grompe M, and Cottle RN
Biology 2025, 14(8), 1091; doi.org/10.3390/biology14081091

Live Cell Culture

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416