Leica LMD6 & LMD7 Laser Microdissection Microscopes

Dissection Perfection

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Preserved striatal innervation maintains motor function despite severe loss of nigral dopaminergic neurons
Paß T, Ricke KM, Hofmann P, Chowdhury RS, Nie Y, Chinnery P, Endepols H, Neumaier B, Carvalho A, Rigoux L, Steculorum SM, Prudent J, Riemer T, Aswendt M, Liss B, Brachvogel B, Wiesner RJ
Brain. 2024 Apr 4:awae089. doi: 10.1093/brain/awae089.

Association between RMTg Neuropeptide Genes and Negative Effect during Alcohol Withdrawal in Mice
Fu Y, Li W, Mai Y, Guan J, Ding R, Hou J, Chen B, Cao G, Sun S, Tang Y, and Fu R
Int. J. Mol. Sci. 2024, 25(5), 2933; Doi: 10.3390/ijms25052933

Digital PCR for Single-Cell Analysis
Fang W, Liu X, Maiga M, Cao W, Mu Y, Yan Q, and Zhu Q
Biosensors 2024, 14(2), 64

DNA Research

Steroids-producing nodules: a two-layered adrenocortical nodular structure as a precursor lesion of cortisol-producing adenoma
Fukumoto T, Umakoshi H, Iwahashi N, Ogasawara T, Yokomoto-Umakoshi M, Kaneko H, Fujita M, Uchida N, Nakao H, Kawamura N, Matsuda Y, Sakamoto R, Miyazawa T, Seki M, Eto M, Oda Y, Suzuki Y, Ogawa S, Ogawa Y
EBioMedicine. 2024 Mar 29:105087. doi: 10.1016/j.ebiom.2024.105087

Proteogenomic analysis of enriched HGSOC tumor epithelium identifies prognostic signatures and therapeutic vulnerabilities
Bateman NW, Abulez T, Soltis AR, McPherson A, Choi S, Garsed DW, Pandey A, Tian C, Hood BL, Conrads KA, Teng PN, Oliver J, Gist G, Mitchell D, Litzi TJ, Tarney CM, Crothers BA, Mhawech-Fauceglia P, Dalgard CL, Wilkerson MD, Pierobon M, Petricoin EF, Yan C, Meerzaman D, Bodelon C, Wentzensen N, Lee JSH; APOLLO Research Network; Huntsman DG, Shah S, Shriver CD, Phippen NT, Darcy KM, Bowtell DDL, Conrads TP, Maxwell GL
NPJ Precis Oncol. 2024 Mar 13;8(1):68. doi: 10.1038/s41698-024-00519-8.

Can endometrial cytology identify patients who would benefit from immunotherapy?
Yanazume S, Kirita Y, Kobayashi Y, Kitazono I, Akahane T, Mizuno M, Togami S, Tanimoto A, Kobayashi H
Acta Cytol. 2024 Mar 12. doi: 10.1159/000538288.

RNA Research

The microbiota and T cells non-genetically modulate inherited phenotypes transgenerationally
Harris JC, Trigg NA, Goshu B, Yokoyama Y, Dohnalová L, White EK, Harman A, Murga-Garrido SM, Ting-Chun Pan J, Bhanap P, Thaiss CA, Grice EA, Conine CC, Kambayashi T
Cell Rep. 2024 Apr 3;43(4):114029. doi: 10.1016/j.celrep.2024.114029.

Synaptically-targeted long non-coding RNA SLAMR promotes structural plasticity by increasing translation and CaMKII activity
Espadas I, Wingfield JL, Nakahata Y, Chanda K, Grinman E, Ghosh I, Bauer KE, Raveendra B, Kiebler MA, Yasuda R, Rangaraju V, Puthanveettil S
Nat Commun. 2024 Mar 27;15(1):2694. doi: 10.1038/s41467-024-46972-8.

LCM-RNAseq highlights intratumor heterogeneity and a lncRNA signature from archival tissues of GH-secreting PitNETs
Cis L, Nanni S, Gessi M, Bianchi A, De Martino S, Pecci V, Bonvissuto D, Carlino A, Giacò L, Rindi G, Sette C, Grassi C, Gaetano C, Pontecorvi A, Farsetti A
Research Square, 19th March 2024

Highlights 2023: SPARCS (spatially resolved CRISPR screening)
Schmacke et al.
https://doi.org/10.1101/2023.06.01.542416

Proteomics, Lipidomics, Metabolomics

An electrostatic cluster guides Aβ40 fibril formation in sporadic and Dutch-type cerebral amyloid angiopathy
Fu Z, Crooks EJ, Irizarry BA, Zhu X, Chowdhury S, Van Nostrand WE, Smith SO
J Struct Biol. 2024 Apr 12:108092. doi: 10.1016/j.jsb.2024.108092.

Spatial omics reveals molecular changes in focal cortical dysplasia type II
Vermeulen I, Rodriguez-Alvarez N, François L, Viot D, Poosti F, Aronica E, Dedeurwaerdere S, Barton P, Cillero-Pastor B, Heeren RMA
Neurobiol Dis. 2024 Apr 2:106491. doi: 10.1016/j.nbd.2024.106491.

Mtb-Selective 5-Aminomethyl Oxazolidinone Prodrugs: Robust Potency and Potential Liabilities
Boshoff HIM, Young K, Ahn YM, Yadav VD, Crowley BM, Yang L, Su J, Oh S, Arora K, Andrews J, Manikkam M, Sutphin M, Smith AJ, Weiner DM, Piazza MK, Fleegle JD, Gomez F, Dayao EK, Prideaux B, Zimmerman M, Kaya F, Sarathy J, Tan VY, Via LE, Tschirret-Guth R, Lenaerts AJ, Robertson GT, Dartois V, Olsen DB, Barry CE 3rd
ACS Infect Dis. 2024 Apr 6. doi: 10.1021/acsinfecdis.4c00025.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Fluorescence Applications

DMHPpp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication
Tokizane K, Brace CS, Imai SI
Cell Metab. 2024 Jan 1:S1550-4131(23)00462-X. doi: 10.1016/j.cmet.2023.12.011.

Cancer Research

Quantitative proteomic analysis of HER2 protein expression in PDAC tumors
Randall J, Hunt AL, Nutcharoen A, Johnston L, Chouraichi S, Wang H, Winer A, Wadlow R, Huynh J, Davis J, Corgiat B, Bateman NW, Deeken JF, Petricoin EF, Conrads TP, Cannon TL
Clin Proteomics. 2024 Mar 20;21(1):24. doi: 10.1186/s12014-024-09476-7.

Neurosciences

Clinical/Pathology/Immunology Research Applications

Here, There, and Everywhere: Somatic Genomics Uncovers DNA Variants to Inspire Drug Discovery
Grinstein JD
GEN Biotechnology, Volume 3, Number 2, 2024 ©Mary Ann Liebert, Inc.

Normalizing granuloma vasculature and matrix improves drug delivery and reduces bacterial burden in tuberculosis-infected rabbits
Datta M, Via LE, Dartois V, Weiner DM, Zimmerman M, Kaya F, Walker AM, Fleegle JD, Raplee ID, McNinch C, Zarodniuk M, Kamoun WS, Yue C, Kumar AS, Subudhi S, Xu L, Barry CE 3rd, Jain RK
Proc Natl Acad Sci U S A. 2024 Apr 2;121(14):e2321336121. doi: 10.1073/pnas.2321336121.

Developmental Research, Epigenetics & Aging

PIBF1 regulates trophoblast syncytialization and promotes cardiovascular development
Lee JG, Yon JM, Kim G, Lee SG, Kim CY, Cheong SA, Kim HY, Yu J, Kim K, Sung YH, Yoo HJ, Woo DC, Rho JK, Ha CH, Pack CG, Oh SH, Lim JS, Han YM, Hong EJ, Seong JK, Lee HW, Lee SW, Lee KU, Kim CJ, Nam SY, Cho YS, Baek IJ
Nat Commun. 2024 Feb 19;15(1):1487. doi: 10.1038/s41467-024-45647-8.

Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling
Costes C, Navarro Sanz S, Calatayud C, Soriano A, Mameri H, Terrier N, Francin-Allami M
BMC Genom Data. 2024 Feb 7;25(1):14. doi: 10.1186/s12863-024-01198-x.

Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction
Gao M, Zhang T, Chen T, Chen Z, Zhu Z, Wen Y, Qin S, Bao Y, Zhao T, Li H, Liu L, Hao M, Wang J, Wang F, Wang H, Zhou B, Zhang H, Xia G, Wang C
Theranostics. 2024 Jan 27;14(4):1371-1389. doi: 10.7150/thno.89878. eCollection 2024.

Plant Research

Transcriptomic analyses to summarize gene expression patterns that occur during leaf initiation of Chinese cabbage
Sun XX, Liu Z, Liu R, Bucher J, Zhao J, Visser RGF, Bonnema G:
Horticulture Research, 2024

Cell-type proteomic and metabolomic resolution of early and late grain filling stages of wheat endosperm
Zhang S, Ghatak A, Mohammad Bazargani M, Kramml H, Zang F, Gao S, Ramšak Ž, Gruden K, Varshney RK, Jiang D, Chaturvedi P, Weckwerth W
Plant Biotechnol J. 2023 Dec 4. doi: 10.1111/pbi.14203.

Forensics

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J
BioMed Research International, Volume 2021 | Article ID 7269237

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics:
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Survival and rapid resuscitation permit limited productivity in desert microbial communities
Imminger S, Meier DV, Schintlmeister A, Legin A, Schnecker J, Richter A, Gillor O, Eichorst SA, Woebken D
Nat Commun. 2024 Apr 17;15(1):3056. doi: 10.1038/s41467-024-46920-6.

Laser Microdissection Protocols for Tissue and Cell Isolation
Multiple
Bio-protocol Selections | 2024

Accelerating Drug Development Using Spatial Multi-omics
Goodwin RJA, Platz SJ, Reis-Filho JS, Barry ST
Cancer Discov. 2024 Apr 4;14(4):620-624. doi: 10.1158/2159-8290.CD-24-0101.

Live Cell Culture

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416

An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022

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