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Correlating Cryofixed Biological Samples in the Light- and Electron Microscope Leica EM Cryo CLEM

  • New hardware and workflowss for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography 
    http://www.sciencedirect.com/science/article/pii/S1047847716301356
  • New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy tomography, Martin Schorba, b, Leander Gaechterc, Ori Avinoama, Frank Sieckmannd, Mairi Clarkea, Cecilia Bebeacuaa, e, Yury S. Bykova, Andreas F.-P. Sonnena, f, Reinhard Lihlg, John A.G. Briggsa, e, f,, a Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany, b Electron Microscopy Core Facility, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany, c Leica Microsystems (Schweiz) AG, Max Schmidheiny-Strasse 201, 9435 Heerbrugg, Switzerland, d Leica Microsystems GmbH, Am Friedensplatz 3, 68165 Mannheim, Germany, e Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany, f Molecular Medicine Partnership Unit, EMBL/Universitätsklinikum Heidelberg, Heidelberg, Germany, g Leica Mikrosysteme GmbH, Hernalser Hauptstraße 219, 1170 Vienna, Austria, http://www.sciencedirect.com/science/article/pii/S1047847716301356
  • Correlative cryo-fluorescence and cryo-scanning electron microscopy as a straightforward tool to study host-pathogen interactions, Martin Strnad1,2, Jana Elsterová1,2,3, Jana Schrenková1,2, Marie Vancová1,2, Ryan O. M. Rego1,Libor Grubhoffer1,2 & Jana Nebesářová1,2,4, 1Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice CZ- 37005, Czech Republic. 2Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760, České Budějovice CZ-37005, Czech Republic. 3Department of Virology, Veterinary Research Institute, Brno CZ-62100, Czech Republic. 4Faculty of Science, Charles University in Prague, Viničná 1594/7, Praha CZ-12800, Czech Republic. Correspondence and requests for materials should be addressed to M.S. (email: martin.strnad.cze(at)gmail(dot)com), 
    https://www.nature.com/articles/srep18029