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Super-Resolution Microscopes and Nanoscopes

Super-resolution microscopes and nanoscopes overcome the diffraction limit of light and allow investigators to study subcellular structures in greater detail than achieved with a standard confocal microscope. With the possibility of resolutions down to 30 nm with STED as well as sub-cellular dynamics can be studied at the nanoscale. Sub-diffraction colocalization analysis reveals interactions unprecedented detail. Enabling novel discoveries to be made in the fields of virology, immunology, neuroscience and cancer, super-resolution is on its way to becoming the new gold standard in light microscopy.

Your entry has never been easier than today with HyVolution 2 and Leica TCS SP8 STED ONE, STED 3X.

Explore life’s true nature in unprecedented detail.

Need Assistance?

Contact a local specialist for advice on the right super-resolution or nanoscopy system for your needs.

HyVolution 2: fast super-resolution for every task

HyVolution 2 is super-resolution for every specimen and every experiment. Study the rapid dynamics of living cells, colocalization, image multiple fluorophores simultaneously, or capture intracellular details with resolution down to 140 Nanometer.

Multiple colors in up to five channels simultaneously

Unlike super-resolution systems that use a single external detection channel HyVolution 2 integrates seamlessly with all confocal imaging modalities. Therefore, you can record all five channels simultaneously – without the need for sequential scanning, which would only slow down the acquisition speed.

Seamless integration with Huygens by SVI: you have the freedom to use fast parallel processing on GPU. HyVolution 2 thus becomes part of the image acquisition process making it the fastest confocal super-resolution system in every respect.

Developed with our partners from SVI.

Pulling the veil from your images: HyVolution 2 reveals minute detail in this 4-channel recording of Cos-7 cells, BodipyFL Phallacidin (Actin), Alexa Fluor 568 F(ab')2 (Tubulin), Alexa Fluor 647 F(ab')2 (Mitochondria), DAPI (nuclei). Sample courtesy of Dr. Jana Döhner, Center for Microscopy and Image Analysis, University of Zürich.

Live cell STED imaging with the new HC PL APO 93X/1.30 GLYC motCORR – STED WHITE. Cytoskeleton in living HeLa cell. Xyz stack with 62 slices confocal/STED planes. SiR tubulin (available from Depth color-coded.

Leica STED: your first choice for nanoscopy

Molecular interaction occurs on the nanoscale, so it is intrinsically difficult to discover with traditional confocal imaging. STED opens the door to the nanoscale, thus providing new insights into molecular interactions. Colocalization, a pre-requisite for the existence of molecular interactions, can be quantified reliably.

The fully integrated STED (STimulated Emission Depletion) systems by Leica Microsystems meet the requirements of daily research and open the door to fast, intuitive, and purely optical access to structural details far beyond the diffraction limit. Resolution becomes tunable in x,y and z with the Leica TCS SP8 STED 3X and TCS SP8 STED ONE.

Nanoscopy in 3D

  • Tunable, direct nanoscopy in x, y and z
  • Multicolor colocalization studies beyond limits
  • Live cell imaging with standard fluorescent proteins like GFP
  • Intuitive software control with Smart STED Wizard
  • Real turnkey due to one-click auto alignment

You decide which level of detail answers your question

Driven by your research: twelve years of innovation towards nanoscopy

True to the credo of Ernst Leitz ‘with the user, for the user’, Leica Microsystems has pioneered super-resolution/nanoscopy right form the beginning by collaborating closely with leading scientists such as the Nobel Prize laureate Stefan Hell, as well as partners in industry. This approach continuously improves the performance of our systems and has won multiple awards (e.g. 2014: R&D 100 and The Scientist Top Ten Innovations awards for TCS SP8 STED 3X; the Microscopy TODAY Innovation Award for SR GSD 3D). With well over a decade of providing solutions in this field, Leica Microsystems today offers not only excellent products but also high standards of service and support.


11. October 2017 White Blood Cell Recruitment to Bacterial Infection

Zebrafish In Vivo Study Using Leica Fluorescence Microscopes

Tile scan image of Mouse brain tissue section with multicolor immunofluorescent labeling

23. May 2017 Find the Missing Links in Advanced Live Cell Research - Leica launches DMi8 S live cell imaging solution

Wetzlar, Germany – Leica introduces the DMi8 S, a new complete solution designed to give researchers the ultimate tool for fast, versatile microscopic imaging of living cells allowing scientists to find, observe and interact with living cells like...

11. April 2017 Full Spectral Freedom for Multi-color Deep Tissue In Vivo Imaging

Leica Microsystems launches SP8 DIVE – The World's First Tunable Deep Imaging Solution

01. November 2016 Explore life’s true nature with super-resolution and nanoscopy

With HyVolution 2 and the Leica TCS SP8 STED ONE, Leica Microsystems paves the way to access imaging beyond the diffraction limit

21. May 2015 GSD Around the World – A Travel Diary on Facebook

Tamara Straube and Peter Laskey will take you on a journey around the world: Together with a team of specialists, they visited 10 countries, organized 11 workshops for GSD super-resolution microscopy, imaging and sample preparation, and met more than...

13. March 2015 EMBL Advanced Course – Intensive Super-Resolution Microscopy Course for Localization Microscopy and STED

From 20th to 25th July 2015 the EMBL (European Molecular Biology Laboratory), Heidelberg will be holding a six-day intensive course on the subject of super-resolution microscopy in cooperation with Leica Microsystems.

19. January 2015 New Leica TCS SP8 at Bates College: „Second of its kind in Maine, new microscope is a game-changer“

According to Bates College, the confocal offers new imaging capabilities and supports research in biology, neuroscience, nanotechnology and photophysics.

03. December 2014 Free Infographic: Which Super-Resolution Microscopy Method is Right for You?

This illustrated guide to super-resolution microscopy helps you compare technologies to best fulfill your individual research needs.

The Leica TCS SP8 STED 3X was chosen among The Scientist Top 10 Innovations 2014

01. December 2014 The Scientist Top 10 Innovations Award for Leica TCS SP8 STED 3X

Leica Microsystems’ STED Super-Resolution Microscope Again Awarded for its Innovative Technology

Leica HyD SMD – the universal detector for SMD and imaging.

19. November 2014 Universal Hybrid Detector for Single Molecule Detection and Imaging at SfN and ASCB

Leica HyD SMD - the Optimal Detector for Precise and Reliable SMD data

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