"People really interact during the sessions – this is really important and helps to create a nice atmosphere. I look forward to participating in the 3rd meeting", said Giuseppe Vicidomini, one of the speakers at this year's meeting, who specializes in gated confocal super-resolution techniques at the IIT, Genoa, Italy.
"The meeting enables users of Leica Microsystems ground-breaking super-resolution systems to meet and share experiences – we are happy to provide a platform for our customers to do that, and to continue to support the work they do in research science" says Baba Awopetu, European Marketing Director, Leica Microsystems.
Talk abstract: Insights into plasma membrane organization using STED microscopy
Christian Eggeling, University of Oxford, Weatherall Institute of Molecular Medicine, Oxford, UK
Lipid-lipid and lipid-protein plasma membrane interactions such as the formation of lipid nanodomains (often denoted "rafts") or restrictions of molecular plasmamembrane diffusion by cortical cytoskeleton compartments are considered to play a functional part in a whole range of membrane associated processes. However, the direct and non-invasive observation of such structures in living cells is impeded by the resolution limit of >200 nm of a conventional far-field optical microscope. With the superior spatial resolution of STED nanoscopy with effective focal spot sizes down to 20–30 nm in living cells, it is now possible to directly resolve such membrane heterogeneities, e.g. by imaging and investigating protein nanoclusters on the membrane surface . On the other hand, the combination of STED nanoscopy with tools such as fluorescence correlation spectroscopy (