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Augmented Reality (AR) Fluorescence Image Gallery
What Does 30,000:1 Magnification Really Mean?
Pursuing private foundation and corporate grant opportunities in Life Science Research
Writing the NIH Research (R01) grant: Strategies for Success
Image Gallery: THUNDER Imager
Plant Cell Development and Morphogenesis
How to prepare samples for Stimulated Raman Scattering
An Introduction to Computational Clearing
Exclusive Aesthetics of Nature
Beware of "Empty" Magnification

Article Sitemap Microscopy

SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM
Development of fluorescence lifetime imaging microscopy (FLIM) and its relevance for functional imaging
How FRET/FLIM Biosensors and Lifetime Imaging Helped Identify the Importance of Zinc for Human B-Cell Development
Single-residue bioorthogonal labeling of G protein-coupled receptors (GPCRs) in live cells for quantitative analysis
Phasor Analysis for FLIM (Fluorescence Lifetime Imaging Microscopy)
Researchers use Functional Imaging to Elucidate the Role of Cellular Zn²⁺ in Human Immunity
FLIM FRET and Biosensors: Versatile Tools for Biomedical Research
Lifetime – a Proper Alternative
Individual Macromolecule Motion in a Crowded Living Cell
FRAP with TCS SP8 Resonant Scanner
Correcting Confocal Acquisition to Optimize Imaging of Fluorescence Resonance Energy Transfer by Sensitized Emission
A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP Sensor as an Example
ATP Changes the Fluorescence Lifetime of Cyan Fluorescent Protein via an Interaction with His148
A mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range
A New FRAP/FRAPa Method for Three-Dimensional Diffusion Measurements Based on Multiphoton Excitation Microscopy
Fluorescence Recovery after Photobleaching with the Leica TCS SP2
Step by Step Guide for FRAP Experiments
FLCS – Advances in Fluorescence Correlation Spectroscopy
Widefield Calcium Imaging with Calcium Indicator Fura2
Fluorescence Recovery after Photobleaching (FRAP) and its Offspring
Förster Resonance Energy Transfer (FRET)
Quantitative Fluorescence
Label-free FLIM
FRET with FLIM
Fluorescence Correlation Spectroscopy
FRET Sensitized Emission Wizard Confocal
FRET Sensitized Emission Wizard Widefield
Perform Microscopy Analysis for Pathology Ergonomically and Efficiently
How to Sanitize a Microscope
Eyepieces, Objectives and Optical Aberrations
Immersion Objectives: Using Oil, Glycerol, or Water to Overcome some of the Limits of Resolution
Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps
Graphene-Based Microbots for Toxic Heavy Metal Removal and Recovery from Water
Collecting Light: The Importance of Numerical Aperture in Microscopy
Introduction to Widefield Microscopy
Milestones in Incident Light Fluorescence Microscopy
Optimization of the Interplay of Optical Components for Aberration free Microscopy
Factors to Consider When Selecting a Research Microscope
Microscope Resolution: Concepts, Factors and Calculation
Color Infidelity: Why Using a Light Source Incorrectly is Cheating on your Data
Video Talk by Kurt Thorn: The Abbe Diffraction Experiment
Micropolarimetric Analysis of Guard Cell Walls in M. Koenigii versus De Novo Localized Starch Granules in Freshly-Isolated Potato Tuber
Video Talk by Joseph Gall: Early History of Microscopy
Video Talk by Jeff Lichtman: Point Spread Function
Video Talk by Jeff Lichtman: Resolution in Microscopy – Wave Optics and the Diffraction Limit
Image Cytometry
Body in Balance
Video Tutorial: How to Change the Bulb of a Fluorescence Lamp Housing
Video Tutorial: How to Align the Bulb of a Fluorescence Lamp Housing
How to Clean Microscope Optics
Scientific Illustration and Animation for Research Scientists
Polarization Contrast
Photo Effects of Light
Optical Contrast Methods
Phase Contrast
Differential Interference Contrast (DIC)
Beware of "Empty" Magnification
How Sharp Images Are Formed
Integrated Modulation Contrast (IMC)
Optical Microscopes – Some Basics
How to prepare samples for Stimulated Raman Scattering
Step by Step Guide to the Molecular Basics of CARS Microscopy
Stimulated Raman Scattering Microscopy Probes Neurodegenerative Disease
Coherent Raman Scattering (CRS) Microscopy: A vast and growing range of applications revealed
Direct visualization of the arterial wall water permeability barrier using CARS microscopy
Chemical Basis for Alteration of an Intraocular Lens Using a Femtosecond Laser
Coupling of vinculin to F-actin requires Syndecan-4 proteoglycan
Unmask the Hidden without Probes: CARS
CARS Microscopy: Imaging Characteristic Vibrational Contrast of Molecules
Good Vibrations
CARS and Confocal
An Introduction to CARS Microscopy
Coherent Raman Scattering Microscopy Publication List
How FLIM Microscopy helps to detect microplastic pollution
A New World of Confocal Applications with the Next Generation White Light Lasers
Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry
Expanding the frontiers of confocal live cell imaging
Obtain maximum information from your specimen with LIGHTNING
Infection of the novel coronavirus SARS-CoV-2 can be blocked by a human monoclonal antibody
Nanoscopy meets Lifetime: Introducing τ-STED
Explore Innovative Techniques to Separate Fluorophores with Overlapping Spectra
Add an Extra Dimension to Confocal Results with TauSense
How to Uncover Hidden Dimensions in Research with Lifetime Imaging
Learn how to Remove Autofluorescence from your Confocal Images
Improve Workflow in Kidney Research with 3D STED Deep Nanoscopy
Improve 3D Cell Biology Workflow with Digital Light Sheet Microscopy
Pore Scale Visualization of Drainage in 3D Porous Media by Confocal Microscopy
Visualize developmental patterns in Drosophila
Visualize mechanical interactions of Cancer Cells
Zebrafish Brain - Whole Organ Imaging at High Resolution
Resolved Field Number (RFN)
What is a Hybrid Detector (HyD)?
What is a Field-of-View Scanner?
What is a Resonant Scanner?
What is a Spectral Detector (SP Detector)?
What is a Tandem Scanner?
Kaggle Competition for Multi-label Classification of Cell Organelles in Proteome Scale Human Protein Atlas Data
MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)
Which Sensor is the Best for Confocal Imaging?
mTORC1 Promotes Proliferation of Immature Schwann Cells and Myelin Growth of Differentiated Schwann Cells
What is FLIM - Fluorescence Lifetime Imaging?
SP FLIM - Spectral Fluorescence Lifetime Imaging
FLIM FRET - Förster Resonance Energy Transfer
FLCS - Fluorescence Lifetime Correlation Spectroscopy
FCCS - Fluorescence Cross-Correlation Spectroscopy
FCS - Fluorescence Correlation Spectroscopy
KChIP2 regulates the cardiac Ca²⁺ transient and myocyte contractility by targeting ryanodine receptor activity
Beam Splitting
Confocal Excitation
Spectral Imaging
Gates Open for Improved Confocal Fluorescence and Super-Resolution STED
Pinhole Effect in Confocal Microscopes
Five Questions Asked: Prof. Dr. Jacco van Rheenen speaks about the most important considerations when imaging deep into mouse tissue
Primary Beam Splitting Devices for Confocal Microscopes
Human NK Cell Development Requires CD56-mediated Motility and Formation of the Developmental Synapse
Testing the Münch Hypothesis of Long Distance Phloem Transport in Plants
Adeno-associated Viral Vectors do not Efficiently Target Muscle Satellite Cells
The Bimodally Expressed MicroRNA miR‐142 Gates Exit from Pluripotency
From Light to Mind: Sensors and Measuring Techniques in Confocal Microscopy
Switching Roles: The Functional Plasticity of Adult Tissue Stem Cells
Label-free in vivo Imaging of Myelinated Axons in Health and Disease with Spectral Confocal Reflectance Microscopy
Highly Selective Fluorescent and Colorimetric Probe for Live-cell Monitoring of Sulphide Based on Bioorthogonal Reaction
Progressive Glucose Stimulation of Islet Beta Cells Reveals a Transition From Segregated to Integrated Modular Functional Connectivity Patterns
"Leica is always flexible and dynamic" - Interview with Audrey Salles, Pasteur Institute, Paris
Video: High Speed Scanning – With two Scanners in one System
How to Choose the Right Confocal Microscope for Your Lab?
Apical Cargo Traverses Endosomal Compartments on the Passage to the Cell Surface
ICln: A New Regulator of Non-Erythroid 4.1R Localisation and Function
Smart Control for Resonant Galvo Scanners
Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis
Establishment and Characterization of an Omasal Epithelial Cell Model Derived from Dairy Calves for the Study of Small Peptide Absorption
Colonization of Potato Rhizosphere by GFP-Tagged Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44 Shown on Large Sections of Roots Using Enrichment Sample Preparation and Confocal Laser Scanning Microscopy
Genomic Survey, Gene Expression Analysis and Structural Modeling Suggest Diverse Roles of DNA Methyltransferases in Legumes
Evolutionary Morphology: Rostock Scientists Clarify Phylogenetic Relationships within the Most Diverse Taxon, Arthropoda
Functional Connectivity in Islets of Langerhans from Mouse Pancreas Tissue Slices
Glucose-Stimulated Calcium Dynamics in Islets of Langerhans in Acute Mouse Pancreas Tissue Slices
The Relationship Between Membrane Potential and Calcium Dynamics in Glucose-Stimulated Beta Cell Syncytium in Acute Mouse Pancreas Tissue Slices
Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
A Flip of the Mitotic Spindle has Disastrous Consequences for Epithelial Cells
Organ Regeneration: An Unlikely Fish Tale
Pinhole Geometry: Four Corners are Perfect
Brighter Fluorescence by Resonant Scanning
Characterization of Photosynthetic Biofilms from Roman Catacombs via 3D Imaging and Subcellular Identification of Pigments
Confocal Microscopy - Optical Path
Confocal Optical Section Thickness
Deep Tissue Imaging
Exploring the Concert of Neuronal Activities
From Molecules to Tissues
Lasers for Confocal
Obese and Slim Yeast Cells
Pinhole Controls Optical Slicing
Stem Cell Biology in Cancer Research
4D Photoactivation of pa-GFP in Living Cells Using Two-Photon Excitation Laser Scanning Microscopy
Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
New Standard in Electrophysiology and Deep Tissue Imaging
Mapping Billions of Synapses with Microscopy and Mathematics
Sniffing Out the Secrets of Social Behavior
The Patch-Clamp Technique
Novel Microscopy-Based Screening Method Reveals Regulators of Contact-Dependent Intercellular Transfer
"We can go home and the imaging is done automatically by the Leica HCS A Matrix Screener."
iMSRC: Converting a Standard Automated Microscope into an Intelligent Screening Platform
Fully Automated Tracking of Chloroplasts in Elodea Leaf Cells from 4D Image Data
Cellular Organization of Cortical Barrel Columns is Whisker-specific
Find the Needle in the Haystack
Mosaic Images
How to make a fast Z-stack
How to Adapt Grain Size Analysis of Metallic Alloys to Your Needs
Digital Microscopy with Versatile Illumination and Various Contrast Methods for More Efficient Inspection and Quality Control
What You Always Wanted to Know About Digital Microscopy, but Never Got Around to Asking
Transnational Telepathology Consultations Using a Basic Digital Microscope: Experience in the Italy-Slovenjia INTERREG Project "Patient Without Borders"
What Does 30,000:1 Magnification Really Mean?
Are Your Digital Microscope Measurements Accurate and Reliable?
Using Telecentric Optical Systems to Optimize Industrial Image Accuracy and Reproducibility
Digital Microscopy
3D Visualization of Surface Structures
Trends in Microscopy
How to improve your Biomarker Discovery Workflow with Laser Microdissection
Observing the effect on transcription factor signaling and chromatin landscapes in cells infected with the human coronavirus 229E
How to improve your Alzheimer Protein Analysis with Laser Microdissection
How to improve your RNA Analysis Workflow with Laser Microdissection
Medicinal Use of Qianhu Plant Roots
How to improve your DNA Mutation Analysis Workflow with Laser Microdissection
Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Nanodroplet Processing Platform for Deep and Quantitative Proteome Proﬁling of 10–100 Mammalian Cells
Correlation of Phenotype and Genotype – microscopic samples and their biomolecular backgrounds
MicroRNA Expression Profiling to Identify and Validate Reference Genes for the Relative Quantification of microRNA in Rectal Cancer
Workflows & Protocols: Plant Laser Microdissection
Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research
Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience
Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection
Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis
Total RNA Isolation after Laser-capture Microdissection of Human Cervical Squamous Epithelial Cells from Fresh Frozen Tissue
Identification of Molecular Pathways Facilitating Glioma Cell Invasion In Situ
The Basic Principles of Laser Microdissection
Glucose-Regulated Protein 78 (Grp78) Confers Chemoresistance to Tumor Endothelial Cells under Acidic Stress
Preparation of Laser Microdissected Plant Cell and Tissue Material for Microchemical Analysis
Sorbin and SH3 Domain‐Containing Protein 2 Is Released from Infarcted Heart in the Very Early Phase: Proteomic Analysis of Cardiac Tissues From Patients
Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
Laser Microdissection (LMD) and Fancy Applications
Video: How to Achieve Fast Precise Cutting Lines in Laser Microdissection
Quantitative One-Step Protocol to Detect Transcripts in Laser Microdissected Samples
Stem Cells in the Aging Process – the Signal Protein p53 as "Guardian of the Genome"
K-ATP Channels in Dopamine Substantia Nigra Neurons Control Bursting and Novelty-induced Exploration
Comparison of the RNA Quality of Native Tissue Samples with Tissue Samples after UV Laser Microdissection
Region-Specific Gene Expression in Adult Mouse CNS Tissues
TED Talk of Allan Jones: Mapping the Human Brain
An Introduction to Laser Microdissection
Applications of Laser Microdissection
Users Report on the Relevance of Laser Microdissection for Their Research Results
Laser Microdissection Publication List
The Mitochondrial Hypothesis of Ageing
The Morbus Parkinson Puzzle
Cell Cultures and Laser Microdissection
Consumables for Laser Microdissection
Writing the NIH Research (R01) grant: Strategies for Success
Pursuing private foundation and corporate grant opportunities in Life Science Research
Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex
How can PAULA help streamline the workflow for my wound healing assay?
Confluency Check with PAULA Cell Imager
Solvent immersion imprint lithography
Introduction to Mammalian Cell Culture
Real Time Observation of Neutrophil White Blood Cell Recruitment to Bacterial Infection In Vivo
How to do a Proper Cell Culture Quick Check
Immunotherapy to Combat Cancer: "Sleeping Beauty" – DNA Plasmid-based Gene Transfer System to Modify T Cells
Live-Cell Imaging Evolves to Find New Niches
Nobel Prize 2013 in Physiology or Medicine for Discoveries of the Machinery Regulating Vesicle Traffic
Direct In Vivo Evidence for Tumor Propagation by Glioblastoma Cancer Stem Cells
Imaging Enzymes at Work
Intravital Immunofluorescence for Visualizing the Microcirculatory and Immune Microenvironments in the Mouse Ear Dermis
Molecular Developmental Biology: Norwegian Marine Research Scientists Solve the Mysteries of Evolution
Integrative Open-Source Software for Image Analysis in Biology
Nobel Prize 2012 in Chemistry for Studies of G-Protein-Coupled Receptors
Image Processing for Widefield Microscopy
The New Repository on the Block
Live-cell Imaging Techniques
Nobel Prize 2012 in Physiology or Medicine for Stem Cell Research
Ratiometric Imaging
Introduction to Live-Cell Imaging
Challenges Faced When Manually Rating Non-Metallic Inclusions (NMIs) to Determine Steel Quality
Criteria to Consider When Choosing a Digital Solution for Steel Quality Rating
Top Issues Related to Standards for Rating Non-Metallic Inclusions in Steel
3-Dimensional Imaging of Macroscopic Defects in Aluminum Alloys
Leica is MAD about LIBS
Cleanliness Analysis with a 2-methods-in-1 solution
Rate the Quality of Your Steel: Free Webinar and Report
See the Structure with Microscopy - Know the Composition with Laser Spectroscopy
Metallography – an Introduction
Steel – It All Depends on What's Really Inside
Research for the Optimal Structure
Steel – the All-Rounder That Has to Pass Many Tests
Metallography with Color and Contrast
How to Quantify Label-free Blood Flow
Principles of Multiphoton Microscopy for Deep Tissue Imaging
Chat with Dr. Thomas Mathivet about In Vivo Cardiovascular Imaging of the Brain
Multiphoton imaging bolsters findings on in vitro differentiation of pluripotent stem cell-derived podocyte
High-Resolution 3D Imaging of Whole Organ after Clearing
Multiphoton Microscopy – a Satisfied Wish List
Mission Impossible Accomplished: Tunable Colors for Non-descanning Detection
Laser Beam Shaping for Multicolor Multiphoton Microscopy
Multiphoton Microscopy Publication List
Clearing of Fixed Tissue: A Review from a Microscopist’s Perspective
BABB Clearing and Imaging for High Resolution Confocal Microscopy: Counting and Sizing Kidney Cells in the 21st Century
Chronic Stress in Mice Remodels Lymph Vasculature to Promote Tumour Cell Dissemination
Third Harmonic Generation Microscopy – Label-Free 3D-Tissue Imaging and Blood Flow Characterization
The Environment Makes the Stem Cell
Tracking Glomerular Fate Over Long Time Distances
Developments in Multiphoton Excitation Microscopy
Multi-photon Excitation Microscopy
Order versus Disorder
What is an OPO?
Coronavirus Replication in Host Cells
Study synaptic dynamics with millisecond precision in neurobiology
Workflow Solutions for Industrial Research
Introduction to Ion Beam Etching with the EM TIC 3X
Ultrastructural Preservation and Improved Visualization of Membranes in Primary Bovine Chromaffin Cells
Studying the Microstructure of Natural Polymers in Fine Detail
Expert Knowledge on High Pressure Freezing and Freeze Fracturing in the Cryo SEM Workflow
Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation
Superior Ultrastructural Preservation and Structural Contrast in Drosophila Tissue
Cryo-SEM analysis of UV light stimulated sun screen lotion
High Resolution Array Tomography with Automated Serial Sectioning
Macroscale to Nanoscale Pore Analysis of Shale and Carbonate Rocks
Visualization of DNA Molecules
Interview with Dr. Shigeki Watanabe on Research in Synaptic Membrane Dynamics
Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions
Giardia lamblia - Sample Preparation for Cryo-SEM
Drosophila larvae - Sample Preparation for Cryo-SEM
Carbon Coating for Polymeric Materials
High-Resolution Carbon Coating: How much Carbon is too much?
Each Atom Counts: Protect Your Samples Prior to FIB Processing
Visualization of Membrane Dynamics with Millisecond Temporal Resolution
Ways to Reveal More from your Samples: Ultra-Thin Carbon Films
Ultra-thin Carbon Support Films for Improved STEM-EELS Analysis of Nanoparticles
Cross Sectioning of Oil Shale Rock
Cross Sectioning of Ni/Cu on Steel for EBSD
Cross Sectioning of a Multilayer System - Preparation of a Perfect Sample Surface for EBSD
Cross Section of Solar Cells
Cross Section of an Aluminium Sample for Electron Backscattered Diffraction (EBSD)
Maple (Acer saccharum) Leaves - High Pressure Freezing and Freeze Substitution for TEM
High-Pressure Freezing and Freeze Substitution of Hep-2 Cells Infected with Chlamydia pneumoniae
Commercially Available Hand Creams - Sample Preparation for Cryo-SEM
Freeze-Fracture Replication of Pyramidal Cells
Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) of Yeast
Cryo-SEM Imaging of Latex Paint
Thin Metal Foils with Coatings - Sample Preparation for SEM
Surface Modification of ZnAg Sample - Sample Preparation for SEM
"Shallow Trench Isolation" Structures - Sample Preparation for TEM
Removal of Surface Layers - Sample Preparation for SEM and TEM
Porous Ceramics - Sample Preparation for SEM
Paper Samples - Sample Preparation for SEM
Multilayer Systems with Widely Different Sputter Rates - Sample Preparation for TEM
Metal Films and Sheets - Sample Preparation for TEM
Contrast Enhancement of Polished Cross Sections of Semiconductor Structures - Sample Preparation for SEM
Ion Beam Polishing of Sample Surfaces - Sample Preparation for SEM
In-Containing Compound Semiconductors - Sample Preparation for TEM
Contrast Enhancement of Polycrystalline Metals - Sample Preparation for SEM
Cleaning of Smeared Sample Surfaces - Sample Preparation for SEM
Ceramics - Sample Preparation for TEM
Picea abies (L.) KARST - Sample Preparation for TEM
Cross Sectioning of Copper for Electron Backscattered Diffraction (EBSD)
Cross Sectioning of a Superconductive Wire
Cross Sectioning of CuSn Connector of a Solar Cell
Cross Sectioning of Rubber (Tire)
Cross Sectioning of Painted Concrete
Cross Sectioning of Cadmiumsulphide (CdS) for Cathodoluminescence
Cross Sectioning of Alumina
Structural Study of C. elegans
Cultured Rat Hippocampal Neurons
Wrinkled Giant Hyssop Leaf Protocol
Wall Cress Pod Protocol - Critical Point Drying of Arabidopsis thaliana for SEM
Tobacco Leaf - Critical Point Drying Protocol for SEM
Nematode E. dianae - Critical Point Drying Protocol for SEM
Clawed Frog Nuclear Envelope Protocol
Black Mould Protocol
Bacteria Protocol - Critical Point Drying of E. coli for SEM
Micro-CT of Insect Larva Protocol
Micro-Computed Tomography (micro-CT) of Insect Brain Protocol
Micro-Computed Tomography (micro-CT) of Book Scorpion Musculature
Water Flea Protocol
Human Blood Cells Protocol
Symmetric Synapse - Clathrin Coated Endocytosis Pit in the Postsynaptic Dendrite
Epoxy Resin Embedding of Animal and Human Tissues for Pathological Diagnosis and Research
Arabidopsis thaliana(L.) Accession Col
Immuno - Electron Microscopy of High Pressure Frozen and Freeze Substituted Mouse Heart
Cell Envelope Structure of a Gram-negative Thermotolerant y-Proteobacterium Acidithiobacillus sp., Strain HV2/2 and its Interaction with Pyrite
Electron Microscopy of High Pressure Frozen and Freeze Substituted Arabidopsis Thaliana Root Tips Cells
Cross Sectioning of Basalt Fibres
Improvement of Metallic Thin Films for HR-SEM by Using DC Magnetron Sputter Coater
High Pressure Freezing with Light Stimulation
Triple-beam Ar-Ion-Milling with a Rotary Stage to Decorate Grain Boundaries and Substructures in Rock Salt
TEM Sample Preparation Made Easy - Prepare TEM Specimen by Broad Beam Argon Ion Milling
Electron Microscopy Sample Preparation: “The Future is Cold, Dynamic and Hybrid”
Cryo-electron Microscopy of Tubular Arrays of HIV-1 Gag Resolves Structures Essential for Immature Virus Assembly
A Good Place for Materials Scientists and Mineralogists to prepare their EM Samples
Critical-point Drying for the Preparation of Biological Samples for MicroCT Analysis
High Quality Sample Preparation for EBSD Analysis by Broad Ion Beam Milling
Transmission Electron Microscopy Sample Preparation Protocols for the Ultrastructural Study of Cysts of Free-living Protozoa
Preparation of Small Disk-shaped Samples for Capacitance and Pyroelectric Current Measurements
Immersion Freezing for Cryo-Transmission Electron Microscopy: Applications
Immersion Freezing for Cryo-Transmission Electron Microscopy: Fundamentals
New Insights into Cilia and Flagella by Cryo-EM
Influence of Tissue and Plant Species in the Trafficking of a Recombinant Protein in Plant Cells
Video Tutorials: Filling and Assembling of Different Carriers for High-Pressure Freezing
Recovery of Recombinant Antibody is Affected by Endogenous Protein Interaction in Maize
Electron Tomography of Cryo-Immobilized Plant Tissue: A Novel Approach to Studying 3D Macromolecular Architecture of Mature Plant Cell Walls In Situ
Brief Introduction to Freeze Fracture and Etching
How to Clean a Coater
Brief Introduction to Specimen Trimming
Introduction to Ultramicrotomy
Glycerol Spraying/Platinum Low Angle Rotary Shadowing of DNA with the Leica EM ACE600 e-beam
Partner Lab for EM Sample Preparation in Paris
Analysis of Oil Shale as an Alternative Source of Energy
Triple Ion Beam Cutting of Diamond/Al Composites for Interface Characterization
Brief Introduction to Freeze Substitution
Freeze Substitution of Trypanosoma brucei
Protein Trafficking in Cereal Seeds
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
Brief Introduction to Contrasting for EM Sample Preparation
Thermodynamic Considerations Regarding the LN2 in a High Pressure Freezer
Rapid Immunohistochemical Diagnosis of Tobacco Mosaic Virus Disease by Microwave-assisted Plant Sample Preparation
From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron Microscopy
Brief Introduction to Glass Knifemaking for Electron and Light Microscope Applications
Brief Introduction to Coating Technology for Electron Microscopy
Carbon Thickness Evaluation in Electron Microscopy
Safe High Pressure Freezing of Infectious Microorganisms
Brief Introduction to High-Pressure Freezing
Immersion Freezing of Suspended Particles and Cells for Cryo-Electron Microscopy
Targeting of Peroxisomal Matrix Proteins in the Diatom Phaeodactylum Tricornutum
Brief Introduction to Critical Point Drying
Perusing Alternatives for Automated Staining of TEM Thin Sections
Capturing Neurotransmitter Receptors and Ion Channels
Atomic Force Microscopy Study of a Stretched Impact Copolymer
A Word on Cathodoluminescence
University of Wollongong Electron Microscopy Centre
Dry Ultrathin Sectioning Combined With High Pressure Freezing
How to Turn Microscope Workplaces Ergonomic
What do you Call a Drosophila who Likes to Drink? A Bar Fly!
Investing in Ergonomically Designed Microscope Workplaces Pays Off
Implementing Ergonomic Design for Microscope Workstations
How to create EDOF (Extended Depth of Focus) images
Coming to Grips with Biological Information Through Flexible Organic Electronics: Developing Bendable and Stretchable Biosensors and Device
Work Efficiently in Developmental Biology and Medical Research with Stereo Microscopy: Rodent and Small Animal Surgery
Work Efficiently in Developmental Biology with Stereo and Confocal Microscopy: C. elegans
Work More Efficiently in Developmental Biology With Stereo Microscopy: Zebrafish, Medaka, and Xenopus
Work More Efficiently In Developmental Biology With Stereo Microscopy: Fruit Flies (Drosophila Melanogaster)
Illumination (Lighting) Systems for Stereo Microscopes
Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
New Predatory Cockroaches (Insecta: Blattaria: Manipulatoridae fam.n.) from the Upper Cretaceous Myanmar Amber
Egg-in-Cube: Design and Fabrication of a Novel Artificial Eggshell with Functionalized Surface
Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury
Welcome to "Sandland" – How Microscopy Uncovers the Beauty of Sand
Ground Beetles Shed Light on the Environmental History of High Mountain Regions
Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae
Higher Motivation, Longer Concentration – Ergonomics as a Competitive Advantage
Detection and Characterization of Hepatic Engraftment of Embryonic Stem Derived Cells by Fluorescent Stereomicroscopy
Stereo microscopes with TripleBeam Technology
Taking the Long View
AntWeb Documents the World of Ants
Stereo microscopes in the EU’s Plant Inspection
Even Insect Fragments Throw Light on How Crimes Are Committed: How Forensic Biologist Dr. Mark Benecke Gains Insights
FusionOptics – Combines high resolution and depth of field for ideal 3D optical Images
In the Footsteps of Linnaeus – Microscopic Imaging in Marine Biodiversity Research
One of Our Last Horizons
Key Factors to Consider When Selecting a Stereo Microscope
Exclusive Aesthetics of Nature
Precision That Saves Eyesight
Intraocular Lenses
The History of Stereo Microscopy – Part I
The History of Stereo Microscopy – Part II
The History of Stereo Microscopy – Part III
Getting Sharper 3D Images of Thick Biological Specimens with Widefield Microscopy
New Light Shed on the Nanodomain Organization of the Endoplasmic Reticulum (ER)
Extending Nanoscopy Possibilities with STED and exchangeable fluorophores
Simultaneously Measuring Image Features and Resolution in Live-Cell STED Images
Dynamic Endoplasmic Reticulum
CNSI Publication List
Observing Malaria Infection at the Right Spot in the Human Host
STED Nanoscopy at the forefront of cancer research
Researchers Find a “Digital” Mechanism Behind Neuronal Changes from Learning
Basal Body Positioning and Anchoring in the Multiciliated Cell Paramecium Tetraurelia: Roles of OFD1 and VFL3
Abstracts of the 7th European Super-Resolution User-Club Meeting
Axial Tubule Junctions Control Rapid Calcium Signaling in Atria
Mirror-Enhanced Super-Resolution Microscopy
The Molecular Architecture of Hemidesmosomes as Revealed by Super-Resolution Microscopy
Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex
Co-Orientation: Quantifying Simultaneous Co-Localization and Orientational Alignment of Filaments in Light Microscopy
Visualizing Tropoelastin in a Long-Term Human Elastic Fibre Cell Culture Model
Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles
Abstracts of the 6th European Super-Resolution User-Club Meeting
Practical Guide for Excellent GSDIM Super-Resolution Images
Measuring the 3D STED-PSF with a new Type of Fluorescent Beads
Super-Resolution Optical Microscopy of Lipid Plasma Membrane Dynamics
STED-FLCS: An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics
TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion
The Actin Cytoskeleton Modulates the Activation of iNKT Cells by Segregating CD1d Nanoclusters on Antigen-Presenting Cells
Actin-Dependent Vacuolar Occupancy of the Cell Determines Auxin-Induced Growth Repression
Translation Microscopy (TRAM) for Super-Resolution Imaging
Two-Photon Excitation STED Microscopy with Time-Gated Detection
Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent
4Pi-RESOLFT Nanoscopy
How to Combine STED and CLARITY
Video: Fluorescence is a State of Mind
Super-Resolution Microscopy of the Synaptic Active Zone
Lytic Immune Synapse Function Requires Filamentous Actin Deconstruction by Coronin 1A
Cross-strand Binding of TFAM to a Single mtDNA Molecule Forms the Mitochondrial Nucleoid
Probes that FIT RNA
A Straightforward Approach for Gated STED-FCS to Investigate Lipid Membrane Dynamics
Cortical Actin Networks Induce Spatio-temporal Confinement of Phospholipids in the Plasma Membrane – A Minimally Invasive Investigation by STED-FCS
Localization of HDAC1 Using Super-Resolution STED Microscopy
STED Nanoscopy with Fluorescent Quantum Dots
Quantifying the Resolution of a Leica SR GSD 3D Localization Microscopy System with 2D and 3D Nanorulers
Abstracts of the 5th European Super-Resolution User-Club Meeting
Gated STED Microscopy with Time-gated Single-photon Avalanche Diode
Encoding and Decoding Spatio-Temporal Information for Super-Resolution Microscopy
Interview with Dr. Gertrude Bunt and Prof. Fred S. Wouters on the FOM 2015
Four questions for Professor Stefan Hell on the subject of FOM 2015
STED Nanoscopy: A Glimpse into the Future
Multi-protein Assemblies Underlie the Mesoscale Organization of the Plasma Membrane
A Lipid Bound Actin Meshwork Organizes Liquid Phase Separation in Model Membranes
Pathways to Optical STED Microscopy
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
Universal PAINT – Dynamic Super-Resolution Microscopy
Super-Resolution – On a Heuristic Point of View About the Resolution of a Light Microscope
Multi-Images Deconvolution Improves Signal-to-Noise Ratio on Gated Stimulated Emission Depletion Microscopy
Detailed Morphological Characterisation of Hendra Virus Infection of Different Cell Types Using Super-Resolution and Conventional Imaging
A Bright Dye for Live-Cell STED Microscopy
Multilayered Polyelectrolyte Microcapsules: Interaction with the Enzyme Cytochrome C Oxidase
Interview with Stefan Hell – For me, Pioneering is …
A New Probe for Super-Resolution Imaging of Membranes Elucidates Trafficking Pathways
3D STED (Stimulated Emission Depletion) Microscopy
Nobel Prize in Chemistry for Achievements in Super-Resolution Microscopy
Super-Resolution Microscopy – Get Your Free e-Book for Download
Correlated Optical and Isotopic Nanoscopy
JC Virus Inclusions in Progressive Multifocal Leukoencephalopathy: Scaffolding Promyelocytic Leukemia Nuclear Bodies Grow With Cell Cycle Transition Through an S-to-G2–Like State in Enlarging Oligodendrocyte Nuclei
Abstracts of the 4th European Super-Resolution User-Club Meeting
STED Microscopy of Living Cells – New Frontiers in Membrane and Neurobiology
Nanoscale Protein Diffusion by STED-Based Pair Correlation Analysis
Video Interview with Jean-Luc Vonesch
Video Interview with William Hughes
Video Interview with Timo Zimmermann
Video Interviews with Kees Jalink
Super-Resolution Microscopy Helped to Create the First 3D Model of a Synapse
Super-resolution Molecular and Functional Imaging of Nanoscale Architectures in Life and Materials Science
Video Interview with Stefan Hell, the Inventor of Super-Resolution
Interview with Dr. Yasushi Okada
Video Talk on Super-Resolution: Overview and Stimulated Emission Depletion (STED) Microscopy
Focus on Microscopy 2014 – Abstracts of the Presentations Online Available
Synaptic Vesicle Recycling: Steps and Principles
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
A New Filtering Technique for Removing Anti-Stokes Emission Background in Gated CW-STED Microscopy
GSDIM Publication List
Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips
The Force of the Dark Side – Embedding Media for GSDIM Super-Resolution Localization Microscopy
Super-Resolution Microscopy Gives New Insights into Nuclear Pore Complex Organization
Super-resolution Microscopy and the Third Dimension
Interview with Stefan Hell: Breaking Nanoscale Boundaries
STED Microscopy: A New Chapter in Light Imaging
Towards Real-time Image Deconvolution: Application to Confocal and STED Microscopy
Watching Molecule Movements in Live Cells
Three-Dimensional Super-Resolution GSDIM Microscopy
New Labeling Tools Can Help to Realize the Full Potential of Super-Resolution Microscopy
Nanoscopy in a Living Multicellular Organism Expressing GFP
Single-Wavelength Two-Photon Excitation-stimulated Emission Depletion (SW2PE-STED) Superresolution Imaging
The Fate of Synaptic Vesicle Components upon Fusion
Natural Killer Cell Lytic Granule Secretion Occurs through a Pervasive Actin Network at the Immune Synapse
Dual Channel STED Nanoscopy of Lytic Granules on Actin Filaments in Natural Killer Cells
STED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects
STED Nanoscopy of Actin Dynamics in Synapses deep inside Living Brain Slices
Two-color STED Microscopy of Living Synapses using a Single Laser-beam Pair
In Vivo Labeling Method Using a Genetic Construct for Nanoscale Resolution Microscopy
STED and GSDIM: Diffraction Unlimited Resolution for all Types of Fluorescence Imaging
Mitochondrial DNA Molecules are Packaged Individually
Tubulin Modifications Affect Monolayer Formation and Apical Trafficking in Epithelial Cells
Abstracts of the 3rd European Super-Resolution User-Club Meeting
Abstracts of the 2nd European Super-Resolution User-Club Meeting
The Guide to STED Sample Preparation
Widefield Super-Resolution with GSDIM
Visualization of the Natural Killer Cell Immune Synapse by Super-Resolution Nanoscopy
Sharp Live Images from the Mouse Brain
Videos on STED Microscopy and its Inventor Stefan Hell
A Guide to Super-Resolution Fluorescence Microscopy
Confocal Nanoscopy Goes Multicolor
Super-Resolution GSDIM Microscopy
Restless Receptors
Video Tutorial: How to Optimize Sample Preparation for GSD Microscopy
The Missing Link to the Nanocosm of Life
Subcellular Localization of AKT and Tubulin using Super-Resolution Microscopy
How Can Immunofluorescence Aid Virology Research?
IL-18 cytokine derived from the enteric nervous system is important for intestinal immunity
Microscopy in Virology
Cholesterol Homeostasis Modulates Platinum Sensitivity in Human Ovarian Cancer
Imaging Intracellular Temperature using Fluorescence Lifetime Imaging Microscopy (FLIM)
The Fundamentals and History of Fluorescence and Quantum Dots
Multispectral Phloem-Mobile Probes: Properties and Applications
Chronic Inflammation Under the Microscope
Investigating the Influence of Hormone Signaling Pathways on Mammary Gland Development and the Onset of Breast Cancer
Fluorescent Proteins Illuminate Cell Biology
How to Prepare Your Specimen for Immunofluorescence Microscopy
Dengue Virus Infection of the Aedes aegypti Salivary Gland and Chemosensory Apparatus Induces Genes that Modulate Infection and Blood-Feeding Behavior
Defect Transport Mechanism Leads to Shortened Chromosome Ends
Spectral and Structural Comparison Between Bright and Dim Green Fluorescent Proteins in Amphioxus
Influencing Factors and Applicability of the Viability EMA-qPCR for a Detection and Quantification of Campylobacter Cells from Water Samples
Video Talk by Roger Tsien: Fluorescent Protein Indicators
Video Talk by Roger Tsien: Fluorescent Proteins
Novel Fluorescent Carbonic Nanomaterials for Sensing and Imaging
Multi-Wavelength Epi-Illumination in Fluorescence Microscopy
Handbook of Optical Filters for Fluorescence Microscopy
Photoactivatable, photoconvertible, and photoswitchable Fluorescent Proteins
Fluorescent Dyes
Basic Principles of Luminescence
Fluorescent Proteins – Introduction and Photo Spectral Characteristics
Modern Fluorescent Proteins and their Biological Applications
Fluorescence in Microscopy
An Introduction to Fluorescence
Improved tree-ring visualization using autofluorescence
Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy
TIRF Publication List
Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results
Total Internal Reflection Fluorescence (TIRF) Microscopy
TIRF Microscopy Explores Cellular Transport Processes
Exploring Cell Logistics
TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells
Applications of TIRF Microscopy in Life Science Research
Nanoscale or Microscale Structures Formed in Polymers Containing Nanotubes Greatly Enhance the Electrical Conductivity: Potential Applications for Photovoltaic Devices
Characterization of Thin Films Using High Definition Confocal Microscopy
Anti-slip Treatment on Glazed Ceramic Tiles: Microstructural and Topographic Modifications
Automated Digital 3D Topography Measurement with the Leica DCM 3D Dual Core Measuring Microscope
An Innovative Non-contact Method to Determine Surface Free Energy on Micro-areas
Surface Roughness of Flowable Resin Composites eroded by Acidic and Alcoholic Drinks
The Effects of Peri-implantitis Decontamination Treatments on the Surface Roughness and Chemistry of a Titanium Alloy Used for Dental Implants: Implications for Bone Reintegration
Brief Introduction to Surface Metrology
No Defect Goes Undetected - Inspection of Micro-optic Components