Rolf T. Borlinghaus , Dr.
Rolf Borlinghaus was born 1956 in Grötzingen, Germany. After his diploma in Biology he worked on electrogenic steps of the Na/K-ATPase by laser-induced release of ATP from a caged compound at Peter Läuger’s Laboratory in the Biophysics Department, University Konstanz, Germany from where he was promoted to Dr.rer.nat. in 1988. He started working as a Product Manager for research Fluorescence and confocal Microscopes with Carl Zeiss, Oberkochen in 1990 and continued to tackle this challenge at Leica in 1997 (at that time Leica Lasertechnik, Heidelberg). For personal insights, in 2007, Rolf Borlinghaus dispensed his managerial responsibilities and is now supporting the confocal marketing group as senior scientist in a half-time position. The other half is dedicated to relations, food and botany.
Springer publications:
2017 Die Lichtblattmikroskopie (DE)
2017 The White Confocal (EN)
2016 Konfokale Mikroskopie in Weiß (DE)
2016 Unbegrenzte Lichtmikroskopie (DE)
Rolf Borlinghaus has retired in December 2020.
Getting Sharper 3D Images of Thick Biological Specimens with Widefield Microscopy
Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…Read article
Drosophila Testis Niche Stem Cells – Three Color Computational Clearing
Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only…Read article
Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex
Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the…Read article
Cellular Motility: Microtubules, Motor Proteins and Tau-Proteins
Cellular motility is based on motor-proteins that can bind to filamentous scaffold proteins and – under consumption of ATP – can “crawl” on these filaments. This note is about proteins connected to…Read article
Alzheimer Plaques: fast Visualization in Thick Sections
More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…Read article
Visualize developmental patterns in Drosophila
The marvelous complexity of life is perhaps the greatest source of fascination within the biological sciences. Among the most complex subjects within biology is the development of multicellular life.…Read article
Visualize mechanical interactions of Cancer Cells
Cells interact with their environment. Not only on the base of chemical signals, but also by sensing and modifying mechanical properties of the extracellular matrix. The research goal of Dr. Bo Sun’s…Read article
Zebrafish Brain - Whole Organ Imaging at High Resolution
Structural information is key when one seeks to understand complex biological systems, and one of the most complex biological structures is the vertebrate central nervous system. To image a complete…Read article
What is a Resonant Scanner?
A resonant scanner is a type of galvanometric mirror scanner that allows fast image acquisition with single-point scanning microscopes (true confocal and multiphoton laser scanning). High acquisition…Read article
What is a Spectral Detector (SP Detector)?
The SP detector from Leica Microsystems denotes a compound detection unit for point scanning microscopes, in particular confocal microscopes. The SP detector splits light into up to 5 spectral bands.…Read article
Resolved Field Number (RFN)
The field number (FN) for optical microscopes indicates the field of view (FOV). It corresponds to the area in the intermediate image that is observable through the eyepieces. Although, we cannot…Read article
What is a Hybrid Detector (HyD)?
A HyD is a sensor for detecting light (photons). It is a compound (hybrid) of two technologies: vacuum tubes, like those used in a photomultiplier tube (PMT), and semiconductor microelectronics, like…Read article
What is a Field-of-View Scanner?
A field-of-view scanner is an assembly of galvanometric scanning mirrors used in single-point confocal microscopes that offer the correct optical recording of large field sizes. The field-of-view…Read article
What is a Tandem Scanner?
A Tandem Scanner is an assembly of two different types of scanning together in one system for true confocal point scanning. The Tandem Scanner consists of a three-mirror scanning base with the…Read article
Which Sensor is the Best for Confocal Imaging?
The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.Read article