Emily M. Mace , Ph.D.
Dr. Emily Mace studies human natural killer cell development, particularly with quantitative image analysis and cell biological approaches. This includes the use of highly spatially and temporally resolved and super-resolution microscopy to understand interactions between NK cell precursors and the microenvironment. She also identifies novel requirements for human NK cell development through the identification and study of rare patients with NK cell deficiencies. This has included the characterization of NK cell functional and cell biological phenotypes associated with MCM10, GATA2, IRF8 and Coronin 1A deficiencies. She is an Assistant Professor of Pediatrics at Columbia University Irving Medical Center in New York. Her work is funded by the National Institutes of Health-National Institute of Allergy and Infectious Diseases.
Regulators of Actin Cytoskeletal Regulation and Cell Migration in Human NK Cells
Dr. Mace will describe new advances in our understanding of the regulation of human NK cell actin cytoskeletal remodeling in cell migration and immune synapse formation derived from confocal and…Read article
Human NK Cell Development Requires CD56-mediated Motility and Formation of the Developmental Synapse
While distinct stages of natural killer (NK) cell development have been defined, the molecular interactions that shape human NK cell maturation are poorly understood. Here we define intercellular…Read article
Lytic Immune Synapse Function Requires Filamentous Actin Deconstruction by Coronin 1A
Natural killer (NK) cells are cytolytic effector cells of the innate immune system. Here, we show that deconstruction of synaptic cortical filamentous (F)-actin by Coronin 1A (Coro1A) is required for…Read article
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse…Read article
Visualization of the Natural Killer Cell Immune Synapse by Super-Resolution Nanoscopy
Natural killer (NK) cells are innate immune effectors that recognize and kill virally infected and tumorigenic cells. Central to their effector function is the formation of an immunological synapse…Read article
Dual Channel STED Nanoscopy of Lytic Granules on Actin Filaments in Natural Killer Cells
Natural killer (NK) cells are innate immune effectors that eliminate diseased and tumorigenic targets through the directed secretion of specialized secretory lysosomes, termed lytic granules. This…Read article