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Sebastian Bänfer

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Sebastian Bänfer studied Biomedical Science at the Philipps-University Marburg, Germany. During his studies he focused on Biochemistry and Cell Biology. After his bachelor thesis "Identification of endosomal compartments using super-resolution light microscopy" he was appointed in 2013 to the so-called "fast track"-program allowing him to start directly with the work on his PhD-project after four years of university studies. He is currently working at the Institute of Cytobiology and Cytopathology. He is also coordinator of the bioimaging facility of the Collaborative Research Center 593 "Mechanisms of Cellular Compartimentalization". His research is focused on intracellular transport mechanisms by the use of super-resolution light microscopy. Sebastian Bänfer is a member of the research group of Prof. Dr. Ralf Jacob.

  • The Force of the Dark Side – Embedding Media for GSDIM Super-Resolution Localization Microscopy

    Super-resolution microscopy such as Stimulated Emission Depletion (STED) and single-molecule based techniques rely on the same principle for breaking the diffraction limit: the unwanted fluorescence signals are switched off during the image acquisition process. Consequently, Ground State Depletion followed by Individual Molecule Return (GSDIM) microscopy and related techniques like PALM, STORM and dSTORM use metastable dark states of a fluorophore for temporal separation of single molecules.
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  • Three-Dimensional Super-Resolution GSDIM Microscopy

    With the new 3D GSDIM technique structures like the Golgi and the microtubular network are resolved not only laterally, but also in a third dimension. The principle is based on the use of optical astigmatism to determine the accurate lateral and axial position of individual fluorochromes.
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