Rolf T. Borlinghaus, Dr.
Rolf Borlinghaus was born 1956 in Grötzingen, Germany. After his diploma in Biology he worked on electrogenic steps of the Na/K-ATPase by laser-induced release of ATP from a caged compound at Peter Läuger’s Laboratory in the Biophysics Department, University Konstanz, Germany from where he was promoted to Dr.rer.nat. in 1988. He started working as a Product Manager for research Fluorescence and confocal Microscopes with Carl Zeiss, Oberkochen in 1990 and continued to tackle this challenge at Leica in 1997 (at that time Leica Lasertechnik, Heidelberg). For personal insights, in 2007, Rolf Borlinghaus dispensed his managerial responsibilities and is now supporting the confocal marketing group as senior scientist in a half-time position. The other half is dedicated to relations, food and botany.
Springer publications:
2017 Die Lichtblattmikroskopie (DE)
2017 The White Confocal (EN)
2016 Konfokale Mikroskopie in Weiß (DE)
2016 Unbegrenzte Lichtmikroskopie (DE)
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Which Sensor is the Best for Confocal Imaging?
The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.Read article -
FLIM FRET and Biosensors: Versatile Tools for Biomedical Research
Fluorescence Lifetime Imaging (FLIM) in combination with Förster Resonance Energy Transfer (FRET) has proven to be very beneficial for investigations in biomedical research for a wide range of…Read article -
Lifetime – a Proper Alternative
„Way too complicated!“ - the notorious feedback when it comes to fluorescence lifetime measurements. This will change now! New technologies and new concepts for data evaluation, all implemented in…Read article -
Multiphoton Microscopy – a Satisfied Wish List
The colorful picture shows colon tumor cells, fluorescently labelled and lineage traced from a multicolor tracer. The gray color codes for the second harmonic generation (SHG) signal from Collagen 1.…Read article -
Mission Impossible Accomplished: Tunable Colors for Non-descanning Detection
Leica Microsystems’ 4Tune detector, the key component of the SP8 DIVE Deep In Vivo Explorer, provides spectrally tunable image recording with non-descanning detection. An innovative solution for…Read article -
Laser Beam Shaping for Multicolor Multiphoton Microscopy
Multiphoton Microscopy is one of the current hot topics in life science research. The new Leica TCS SP8 DIVE from Leica Microsystems presents a series of beneficial new innovations, including a freely…Read article -
Primary Beam Splitting Devices for Confocal Microscopes
Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device performing this separation is a color-dependent beam…Read article -
Pinhole Effect in Confocal Microscopes
When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to…Read article -
HyVolution – Super-Resolution Imaging with a Confocal Microscope
Since the invention of the microscope, there has been continual discussion about the possibility of showing more detailed features of specimens as compared to just magnifying them. In this article we…Read article -
HyVolution – the Smart Path to Confocal Super-Resolution
Super-resolution refers to any device or method that can resolve better than the classical Abbe limit. Apart from infinite super-resolution techniques such as STED (stimulated emission depletion) and…Read article -
From Light to Mind: Sensors and Measuring Techniques in Confocal Microscopy
This article outlines the most important sensors used in confocal microscopy. By confocal microscopy, we mean "True Confocal Scanning", i.e. the technique that illuminates and measures one single…Read article -
Confocal and Digital Light Sheet Imaging
Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the…Read article -
Super-Resolution – On a Heuristic Point of View About the Resolution of a Light Microscope
Since super-resolution has become one of the most favored methods in biomedical research, the term has become increasingly popular. Still, there is much of confusion about what is super-resolution and…Read article -
Clearing Procedures for Deep Tissue Imaging
Multi-channel multiphoton microscopy with dedicated optics for CLARITY. Why clearing? Curiosity is human nature. And nothing attracts as much curiosity as the inside of living organisms. While in…Read article -
Smart Control for Resonant Galvo Scanners
High time-resolution confocal microscopy (HTRCLSM) requires fast scanning devices. Whereas non-resonant galvo scanners allow full position control, but only at slow speed, resonant scanners allow…Read article -
Super-resolution Microscopy and the Third Dimension
Optical imaging devices have a finite depth of field and diffraction limited resolution. The depth of field problem was tackled first with confocal microscopes, diffraction unlimited resolution is…Read article -
Acousto Optics in True Confocal Spectral Microscope Systems
Acousto-optical elements have successfully replaced planar filters in many positions. The white confocal, regarded as the fully spectrally tunable confocal microscope, was not possible without this…Read article -
Spectral Detection – How to Define the Spectral Bands that Collect Probe-specific Emission
To specifically collect emission from multiple probes, the light is first separated spatially and then passes through a device that defines a spectral band. Classically, this is a common glass-based…Read article -
Multiple Microscopy Modes in a Single Sweep with Supercontinuum White Light
Lasers have been critical to the advancement on confocal microscopy, and the white light laser (WLL) offers particular advantages. Finessing WLL output for bioimaging is a complex task, though, and…Read article -
Pinhole Geometry: Four Corners are Perfect
Square and hexagonal pinholes provide identical image signal levels, if the geometries are compared in a sensible manner. The amount of light passing the pinhole depends on the area of that aperture,…Read article -
Unmask the Hidden without Probes: CARS
Fluorescence microscopy assumed a pivotal role in cell biology once it was possible to stain cell components selectively by fluorescing dyes. One of the first explorers of targeted stainings, Paul…Read article -
STED and GSDIM: Diffraction Unlimited Resolution for all Types of Fluorescence Imaging
This article gives an overview of two different types of superresolution techniques. Stimulated emission depletion (STED) microscopy is a versatile and fast method that is based on point scanning…Read article -
The White Confocal – Spectral Gaps Closed
This article summarizes the development and differences in design and functionality of confocal technology as far as spectral properties are concerned, from classical filter-based excitation and…Read article -
The White Confocal
Modern biomedical research is currently dominated by imaging and measuring with optical microscopes. One branch of the microscopy technology is confocal microscopy. For correlation purposes,…Read article -
Detectors for Sensitive Detection: HyD
This article discusses detectors (more precisely: sensors), that are employed in single point, i.e. true confocal scanning microscopes. The sensors in such systems are usually photomultiplier tubes.…Read article