Jochen J. Sieber, PhD
Jochen Sieber, Product Manager Superresolution Technologies, Leica Microsystems Mannheim, studied biochemistry in Leipzig, Germany and in Glasgow, UK. He acquired his doctor’s degree at the neurobiology department of the Max-Planck-Institute for biophysical chemistry in Göttingen. There, he was exemplarily characterizing the Syntaxin 1-Cluster to acquire further knowledge about the architecture and dynamics of the plasma membrane. The co-operation with Prof. Stefan Hell provided Sieber with the opportunity to determine size, number and density of this nanostructure via STED-Microscopy. In 2007, he became Application Developer Focus Superresolution before becoming Product Manager Superresolution in 2010.
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Measuring the 3D STED-PSF with a new Type of Fluorescent Beads
A new type of fluorescent bead is presented by GATTAquant. These beads, called GATTA-Beads, are characterized by a small diameter (23 nm), high intensity and size uniformity. In combination with state-of the-art STED microscopes such as the Leica TCS SP8 STED 3X and high-end image restoration methods available in the Huygens Software, it is shown that these new beads can be used for accurate STED PSF characterization in 3D. Furthermore, it is shown that the measured 3D STED-PSF can be used to improve image restoration quality in combination with STED deconvolution methods available in the Huygens Software.Read article -
STED Publication List
It is already 20 years ago that STED microscopy was first described by Stephan Hell. In the meantime it has become an important tool to study subcellular structures beyond the diffraction limit. This reference list gives an overview about the technological development of STED nanoscopy and also shows selected publications performed on a Leica STED instrument.Read article -
Depletion and Emission Beam Paths in STED Microscopy
STED is one technology, and it requires already additional beam-routings as compared to pure fluorescence imaging. The Leica TCS SP5 offers configurations with STED, confocal and multiphoton imaging in the very same instrument. This unique concept unites technologies of super-resolution fluorescence, multichannel confocal fluorescence, multiphoton-excited fluorescence and second-harmonic or higher order nonlinear image generation. The various beam-paths are shown and explained in this tutorial.Read article -
Stimulated Emission Depletion (STED)
Among the new super-resolution technologies, stimulated emission depletion (STED) is the most versatile concept. Whether the sample is tissue, e.g. muscle striation details, classical cytoskeletons, nuclear proteins, yeast or bacterial details: STED serves for understanding structure and function by showing the very finest details.Read article -
Beam Paths of a Continuous Wave STED Microscope
This tutorial gives on overview of the excitation of an detection light path with a continuous wave (CW) STED microscope.Read article -
STED Super-Resolution Microscopy (Nanoscopy) - Principles and Photophysics
This tutorial focusses on the principles of STED super-resolution microscopy. The underlying photo physical processes are explained - e.g. with the help of a Jablonski diagram - and the integration into a confocal laser scanning microscope.Read article -
Confocal Nanoscopy Goes Multicolor
Scientists strive to understand the architecture of life. They want to learn how biological structures are arranged in respect to one another. Multicolor superresolution imaging allows fundamental questions to be addressed by far-field fluorescence microscopy in unprecedented detail.Read article