A new type of fluorescent bead is presented by GATTAquant. These beads, called GATTA-Beads, are characterized by a small diameter (23 nm), high intensity and size uniformity. In combination with state-of the-art STED
microscopes such as the Leica TCS
3X and high-end image restoration methods available in the Huygens Software, it is shown that these new beads can be used for accurate STED
PSF characterization in 3D. Furthermore, it is shown that the measured 3D STED
-PSF can be used to improve image restoration quality in combination with STED
deconvolution methods available in the Huygens Software.