Gražvydas Lukinavicius studied Biochemistry at the University of Vilnius in the group of Prof. Saulius Klimašauskas. During PhD studies he investigated the catalytic mechanism of DNA methyltransferases and successfully developed of a new sequence specific DNA labelling method - methyltransferase-directed transfer of activated groups (mTAG). Later, he became interested in protein labelling methods and joined Prof. Kai Johnsson’s group at École Polytechnique Fédérale de Lausanne (EPFL) to undertake postdoctoral research. He, together with chemists Keitaro Umezawa and Luc Reymond, have improved protein and DNA labelling technologies by developing a new biocompatible fluorophore – silicon-rhodamine (SiR). During these developments, he realized the importance of Super-Resolution fluorescence microscopy for studying living cells and started collaborating with Prof. Stefan W. Hell and Leica microsystems to perform his first Super-Resolution microscopy experiments of living cells. Currently, he is working in the Max Planck Institute for Biophysical Chemistry at the department of Nanobiophotonics, where he continues to develop new probes for nanoscopy.