Paolo Bianchini
Paolo Bianchini, born in 1978, obtained his degree in Physics at University of Genoa, Italy, in 2004. In 2008 he received his Ph.D. in Material Sciences from the University of Genoa with a thesis titled “Multimodal characterization of biological/biocompatible systems by means of advanced microscopy methods”.
His research deals with the design, development and utilization of biophysical instrumentation oriented to the study of biological macromolecules‚ i.e. proteins‚ in thick tissues, in vitro and in vivo. In particular he developed an original setup for SW 2PE-STED (single wavelength two-photon excitation stimulated emission depletion) super-resolution microscopy and another for SHG (second harmonic generation) combined with 2PEF (2-photon excitation fluorescence) microscopy. Other interests are the study on biological molecules to address cell functioning using conventional, confocal microscopy, FLIM (Fluorescence Lifetime Imaging Microscopy), single molecule imaging, spectral microscopy, nanostructured model systems, bioimaging. He worked also on a nanotechnological approach for the realization of Nanobiorobot, hybrid nanostructured devices made by living cells and polylelctrolyte layers.
Paolo is active member of international scientific societies and has been selected as reviewer of different international scientific journals: Biophysical Journal, Journal of Biomedical Optics, Microscopy Research and Technique, Photochemical & Photobiological Sciences.
STED Nanoscopy: A Glimpse into the Future
The well-known saying of "Seeing is believing" became even more apt in biology when stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel laureate S. Hell and coworkers.…Read article
Nanoscale Protein Diffusion by STED-Based Pair Correlation Analysis
We describe for the first time the combination between cross-pair correlation function analysis (pair correlation analysis or pCF) and stimulated emission depletion (STED) to obtain diffusion maps at…Read articleSingle-Wavelength Two-Photon Excitation-stimulated Emission Depletion (SW2PE-STED) Superresolution Imaging
Two-photon microscopy, multiphoton microcopy and super-resolution imaging. We developed a new class of two-photon excitation–stimulated emission depletion (2PE-STED) optical microscope. In this…Read article
Order versus Disorder
In modern biomaterial design the generation of an environment mimicking some of the extracellular matrix features is envisaged to support molecular cross-talk between cells and scaffolds during tissue…Read article
Stem Cell Biology in Cancer Research
The comprehension of stem cell biology and its molecular basis is now acquiring paramount importance in cancer research. The need to look at a single, possibly living, cell makes fluorescence…Read article
The First Supercontinuum Confocal that Adapts to the Sample
Until now, biological and medical research fluorescence imaging in multi-user facilities or institutes has been limited by the type or number of dyes that could be excited. The Leica TCS SP5 X…Read article
Multi-photon Excitation Microscopy
Advanced microscopical techique for life science: multiphoton microscopy. Multi-photon excitation (MPE) microscopy plays a growing role among microscopical techniques utilized for studying biological…Read article