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Falk Schlaudraff, Dr.

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Falk Schlaudraff was born in Lüneburg, Germany. After his Master in Molecular Life Science at the University of Lübeck, where he also absolved the undergraduate studies of Computer Science, he moved to Marburg (Phillipps-University Marburg, Germany) and later to Ulm (University of Ulm, Germany), where he worked on and finished his Ph.D. thesis on Parkinsons Disease at the Laboratory of Prof. Birgit Liss. Afterwards he worked as Scientist R&D for Qiagen (Hilden, Germany), before he started at Leica Microsystems as Product Manager in May 2011.

Falk.Schlaudraff(at)leica-microsystems(dot)com

http://schlaudraff.moltkeplatz.de

http://schlaudraff.privat-server.net/joomla/index.php/cv-falk-eng

  • Laser Microdissection Publication List

    This monthly updated reference list demonstrates the major application fields for laser microdissection in life science research.
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  • Correlation of Phenotype and Genotype – microscopic samples and their biomolecular backgrounds

    Seeing is believing, this is what makes microscopes, imaging systems, or box-type imagers so important for researchers in many different fields like pathology research, cancer research, neuroscience, and developmental research. Correlation of phenotype (what you can see) and genotype (genetic background) is highly desired but often biased by whole tissue approaches as no real solution is available to reliably separate the single cells of interest and surrounding tissue. Thus, results of DNA mutation analysis (sequencing), gene expression profiling (quantitative real-time PCR, qPCR, microarray, digital PCR), next generation sequencing (NGS) or mass spectrometry (MS) approaches can give mixed results as no pure starting material for the biomolecular methods of choice is available.
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  • Workflows & Protocols: Plant Laser Microdissection

    During Leica workshops for LMD users in Brazil, hosted by the Federal University of Paraná/UFPR (UFPR) at the Centro de Energia Nuclear na Agricultura/USP (CENA), the power of laser microdissection using the Leica LMD systems was demonstrated. One special focus was on plant dissection which needs a high laser power.
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  • Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research

    Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we report the reason why laser microdissection is a perfect tool for this purpose and how this was taught in the course of a workshop held in Brazil. With the Leica LMD system pure tumor material can be selected and dissected for downstream analysis to ensure 100% pure starting material without any risk of cross contamination with healthy cells.
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  • Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience

    The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is a suitable techniques for brain investigation as it allows to separate distinct brain layers or even to isolate individual neurons.
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  • Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection

    During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome. Another topic was the dissection of individual chromosomes from chromosome spreads. Leica specialists held a short training session with the LMD. After this, new LMD users were able to run the system and practiced how to dissect chromosomes and collect single chromosomes for downstream analysis.
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  • Webinar: Leica Microsystems Laser Microdissection – Dissection Perfection

    Laser Microdissection (LMD) is a microscopic technique for isolating homogeneous, specific and pure targets from heterogeneous samples for downstream analysis (DNA, RNA & proteins). In this webinar you will learn about techniques for precise, contamination-free isolation of specific cell types and obtain an overview of the scientific and practical considerations for obtaining highly pure material for further molecular analysis in the field of Parkinson's disease and plant research.
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  • Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis

    Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or sequencing techniques. Here we describe the successful combination of the Leica Microsystems LMD system and Qiagen kits for purification of nucleic acids even from little amounts.
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  • The Basic Principles of Laser Microdissection

    In contrast to other systems which use a fixed laserfocus for dissection, Leica Microsystems' LMD systems guide the laserfocus for dissection. This unique feature allows highly precise laser dissection independent of the stage accuracy. This tutorial describes the basic principles of the laser guidance and how the laser can be adjusted for perfect cutting results.
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  • The Morbus Parkinson Puzzle

    A characteristic sign of M. Parkinson is the deterioration of dopaminergic neurons in the mid-brain, specifically in the substantia nigra (SN, black substance). Different causes and forms of this disease have been identified. In the case of the genetic familial form, for example, it has been possible to identify various genes that have a causal influence for M. Parkinson.
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  • K-ATP Channels in Dopamine Substantia Nigra Neurons Control Bursting and Novelty-induced Exploration

    Phasic activation of the dopamine (DA) midbrain system in response to unexpected reward or novelty is critical for adaptive behavioral strategies. This activation of DA midbrain neurons occurs via a synaptically triggered switch from low-frequency background spiking to transient high-frequency burst firing. We found that, in medial DA neurons of the substantia nigra (SN), activity of ATP-sensitive potassium (K-ATP) channels enabled NMDA-mediated bursting in vitro as well as spontaneous in vivo burst firing in anesthetized mice.
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