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Jacco van Rheenen, PhD


Dr. Jacco van Rheenen obtained his PhD in the laboratory of Kees Jalink at the Netherlands Cancer Institute in Amsterdam, and during his postdoctoral studies in the laboratory of John Condeelis, he learned how to study tumour cell motility by intravital microscopy. In 2008, he became junior group leader (equals assistant professor) at the Hubrecht Institute in Utrecht, the Netherlands. In 2012, he became senior group leader (equals associate professor) and in 2014 he became full professor of Intravital Microscopy at the University of Utrecht. His laboratory uses intravital microscopy to study various hallmarks of cancer including the role of the immune system, migration, and stemness in the growth and metastasis of tumours.

  • Five Questions Asked: Prof. Dr. Jacco van Rheenen speaks about the most important considerations when imaging deep into mouse tissue

    When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to explain the significance of the pinhole for those, who did not want to spend too much time to dig into theory and details of confocal microscopy but wanted to have an idea about the effect of the pinhole.
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  • The Environment Makes the Stem Cell

    A recent publication in Nature shows that all stem cells divide and compete for niche space by passively "kicking out" others so that eventually one stem cell takes over the whole niche. Jacco van Rheenen and Saskia Ellenbroek talk about a new method of intravital imaging, which allows following the fate of individual stem cells over time in vivo and explains the new paradigm for stem cell development in the intestinal stem cell niche.
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  • Correcting Confocal Acquisition to Optimize Imaging of Fluorescence Resonance Energy Transfer by Sensitized Emission

    Imaging of fluorescence resonance energy transfer (FRET) between suitable fluorophores is increasingly being used to study cellular processes with high spatiotemporal resolution. The genetically encoded Cyan (CFP) and Yellow (YFP) variants of Green Fluorescent Protein have become the most popular donor and acceptor pair in cell biology. FRET between these fluorophores can be imaged by detecting sensitized emission. This technique, for which CFP is excited and transfer is detected as emission of YFP, is sensitive, fast, and straightforward, provided that proper corrections are made. In this study, the detection of sensitized emission between CFP and YFP by confocal microscopy is optimized.
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