CPD300 auto program
Coating
Mount the dried samples on stubs containing carbon adhesives
Platinum / Palladium coating: 6 nm
Black Mould Protocol
Application Note for Leica EM CPD300 - Life Science Research
Introduction
Species: Black mould (Aspergilus niger)
Critical point drying of Black mould with subsequent platinum / palladium coating and SEM analysis to detect conidiospores
Procedure
Sample Holder: Sample was inserted into a filter disc (Pore size: 16 - 40 μm) and placed into the filter discs and porous pots holder
Cultivation
Cultivate fungi on agar containing growth medium for 3 days
Fixation and Dehydration
| 3% Glutaraldehyde in PBS, pH 7.3 at 4°C | 18 h |
| Distilled water | 3x10 min. |
| 1% aqueous OsO4, 4°C | 18 h |
| Distilled water | 3x10 min. |
| Ethanol series: 30%, 50%, 70%, 80%, 90%, 96%, 100% at 25°C | 1x10 min. |
| 1% DMP in Acetone series: 30%, 50%, 100% | 3x30 min. |
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