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Depletion and Emission Beam Paths in STED Microscopy

Modern high-end microscopy requires a multitude of various types of microscope-techniques to be unified in a single system. This allows generating differently contrasted microscopic images. STED is one technology, and it requires already additional beam-routings as compared to pure fluorescence imaging.

The Leica TCS SP5 offers configurations with STED, confocal and multiphoton imaging in the very same instrument. This unique concept unites technologies of super-resolution fluorescence, multichannel confocal fluorescence, multiphoton-excited fluorescence and second-harmonic or higher order nonlinear image generation.

The various beam-paths are shown and explained in this tutorial.


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Read more about STED microscopy

  1. STED Super-Resolution Microscopy - Principles and Photophysics
  2. Beam Path of a Continuous Wave STED Microscope
  3. Stimulated Emission Depletion (STED): a Super-Resolution Technique for Many Uses
  4. Unlimited Resolution: STED
  5. Abstracts of the First European Super-Resolution User Club Meeting