Brains were imaged with the Digital LightSheet (DLS) from Leica Microsystems. It allows the user to choose between several detection objectives and mounting frames enabling high-resolution visualization. Users can also take full advantage of all the structural details revealed within the 3D cleared insect brains.
Bekkouche B.M.B., Fritz H.K.M., Rigosi E. & O'Carroll D.C.:
Comparison of Transparency and Shrinkage During Clearing of Insect Brains Using Media With Tunable Refractive Index
Front. Neuroanat., 20 November 2020, https://doi.org/10.3389/fnana.2020.599282