Two-color, live-cell low power TauSTED 775; cytoskeleton (SPY-620 tubulin, glow) and vesicles (WGA-594, cyan). Scale bar, 5 and 2 μm while zoomed in. Fast dynamics captured at 1 frame/s. Montage shows a selection of time points from the inset; TrackMate Fiji plugin* traces three individual vesicles (red, green and blue). SPY probes available from Spirochrome.
Images acquired on STELLARIS STED with motCorr 86X water STED WHITE objective lens and Power HyD X detectors.
*Tinevez, J. Y. et al. TrackMate: an open and extensible platform for single particle tracking. Methods 115, 80–90 (2017).