Fast 2 colour live cell dynamics with TauSTED gentle super-resolution

TauSTED: pushing STED beyond its limits with lifetime

 tausted-cell-dynamics.jpg

TauSTED, with low photon budget and the fast detection scheme in our STELLARIS confocal platform, enables the tracking of highly dynamic processes in live cells. We can see in this example the dynamics of the cytoskeleton and membrane vesicles. 

Two-color, live-cell low power TauSTED 775;  cytoskeleton (SPY-620 tubulin, glow) and vesicles (WGA-594, cyan). Scale bar, 5 and 2 μm while zoomed in. Fast dynamics captured at 1 frame/s. Montage shows a selection of time points from the inset; TrackMate Fiji plugin* traces three individual vesicles (red, green and blue). SPY probes available from Spirochrome. 

Images acquired on STELLARIS STED with motCorr 86X water STED WHITE objective lens and Power HyD X detectors.

*Tinevez, J. Y. et al. TrackMate: an open and extensible platform for single particle tracking. Methods 115, 80–90 (2017).

pubmed.ncbi.nlm.nih.gov/27713081/

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Further reading

Application note in nature.com: Pushing STED beyond its limits with TauSTED

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