Quantitative parameters derived from cross-correlation curve
The diffusion of double-marked dimers creates synchronized (i.e., correlated) signal fluctuations, while single marked monomers cause independent fluctuations. An increasing amplitude of the cross-correlation curve reflects an enlargement of the bound fraction. Additionally, like in FCS , information about particle concentration, particle mass, viscosity, and bound fraction can be achieved.
Sensitivity
FCCS detects high specifically only bound particles that travel together. In contrast to FCS , binding partner can have the same molecular mass.
