FCCS - Fluorescence Cross-Correlation Spectroscopy

The FCCS Method

FCCS (Fluorescence Cross-Correlation Spectroscopy) can be measured using the Leica TCS SP8 FCS system. Similar to FCS , it analyzes fluorescence intensity fluctuations derived from a small observation volume. In FCCS potential binding partners are marked with spectrally different fluorophores. Their emitted photons are detected in two separate channels.


Topics & Tags

Quantitative parameters derived from cross-correlation curve

The diffusion of double-marked dimers creates synchronized (i.e., correlated) signal fluctuations, while single marked monomers cause independent fluctuations. An increasing amplitude of the cross-correlation curve reflects an enlargement of the bound fraction. Additionally, like in FCS , information about particle concentration, particle mass, viscosity, and bound fraction can be achieved.


FCCS detects high specifically only bound particles that travel together. In contrast to FCS , binding partner can have the same molecular mass.

Related Pages

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting?

  • Leica Microsystems Inc.
    1700 Leider Lane
    Buffalo Grove, IL 60089 United States
    Office Phone : +1 800 248 0123
    Service Phone : 1 800 248 0223
    Fax : +1 847-236-3009

You will find a more detailed list of local contacts here.