Fission Yeast Actomyosin Ring Integrity
Stress-activated MAPK signaling controls it by modulating formin For3 levels
In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging is presented in detail. Cytosolic Ca2+ levels are maintained at low nanomolar concentrations and disruption of Ca2+ homeostasis is associated with cell/tissue damage. Methods have been developed to accurately assess cellular Ca2+ levels, where each has intrinsic advantages and disadvantages. This Fura 2 ratiometric Ca2+ measurement minimizes possible artifacts. The protocol allows evaluation of the immediate effect of chemical addition. Results for this work were obtained using a Leica widefield inverted and fluorescence microscope. For more details, refer to figure 1 and the publication.
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