Fission Yeast Actomyosin Ring Integrity

Stress-activated MAPK signaling controls it by modulating formin For3 levels


In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging is presented in detail. Cytosolic Ca2+ levels are maintained at low nanomolar concentrations and disruption of Ca2+ homeostasis is associated with cell/tissue damage. Methods have been developed to accurately assess cellular Ca2+ levels, where each has intrinsic advantages and disadvantages. This Fura 2 ratiometric Ca2+ measurement minimizes possible artifacts. The protocol allows evaluation of the immediate effect of chemical addition. Results for this work were obtained using a Leica widefield inverted and fluorescence microscope. For more details, refer to figure 1 and the publication.

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E. Gómez-Gil, R. Martín-García, J. Vicente-Soler, A. Franco, B. Vázquez-Marín, F. Prieto-Ruiz, T. Soto, P. Pérez, M. Madrid, J. Cansado

Stress-activated MAPK signaling controls fission yeast actomyosin ring integrity by modulating formin For3 levels

eLife (2020) vol. 9, e57951, DOI: 10.7554/eLife.57951.

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