This approach allowed us to image Ca2+ changes by live confocal microscopy using an inverted TCS SP8 confocal microscope upon acute perfusion. Spheroids of immortalized cells from human taste/tongue papillae (BRAIN Biotech AG) were stimulated with tastants or ATP. Analysing the responses in concentric spheroid regions showed that cells at the rim displayed larger and faster transients than those in the core. These regional differences were confirmed in analogous experiments with light-sheet microscopy, done with a TCS SP8 DLS, which allowed us to image approximately 100 μm in depth without losing spatio-temporal resolution.
Molitor E., Nürnberg E., Ertongur-Fauth T., Scholz P., Riedel K., Hafner M., Rudolf R. & Cesetti T.:
Analysis of Calcium Signaling in Live Human Tongue Cell 3D-Cultures upon Tastant Perfusion
Cell Calcium 87 (May 2020): 102164, DOI: 10.1016/j.ceca.2020.102164. https://www.sciencedirect.com/science/article/abs/pii/S0143416020300063