Gaining Insights into Colorectal Cancer Metastasis

Following metastasis with intravital multiphoton microscopy

To follow cancer metastasis at the point of occurrence intravital multiphoton microscopy is the tool of choice. Intravital microscopy allows researchers to obtain image data from a living animal, even repeatedly over long periods of time. By implanting a window into a part of the animal’s anatomy, such as the abdomen, repeated fluorescence imaging can be accomplished over days and weeks. In this study, the authors used intravital multiphoton microscopy to show how colorectal cancer metastasis is driven by the presence of cells which are negative for the Lgr5 protein (Lgr5- cells). In addition, they found that long term metastatic growth is enabled by Lgr5 being present in the cells (Lgr5+). Results for this study were obtained using a Leica multiphoton microscope. For more details, refer to the publication.



Video: Time-lapse intravital images of a migratory field showing primary tumor cell migration of Lgr5− and cancer cells (ROI, region of interest 1) and Lgr5+ CSCs (ROI2). Dashed lines highlight the migratory cells, continuous lines mark the migratory tracks overtime.

Read the full article:

A. Fumagalli, K.C. Oost, L. Kester, J. Morgner, L. Bornes, L. Bruens, L. Spaargaren, M. Azkanaz, T. Schelfhorst, E. Beerling, M.C. Heinz, D. Postrach, D. Seinstra, A.M. Sieuwerts, J.W.M. Martens, S. van der Elst, M. van Baalen, D. Bhowmick, N. Vrisekoop, S.I.J. Ellenbroek, S.J.E. Suijkerbuijk, H.J. Snippert, J. van Rheenen:

Plasticity of Lgr5-Negative Cancer Cells Drives Metastasis in Colorectal Cancer

Cell Stem Cell (2020) vol. 26, iss. 4, pp. 569-578.E7, DOI: 10.1016/j.stem.2020.02.008.


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