FLIM for high-speed investigation of molecular interactions
FLIM image gallery
SP8 FALCON (FAst Lifetime CONtrast) is the fast and fully integrated platform for fluorescence lifetime imaging (FLIM) and analysis. It enables the observation of fast dynamic cellular events with lifetime contrasts between fluorescent molecules down to sub-second time scales. With the SP8 FALCON platform, it is now possible to measure molecular interactions with Förster Resonance Energy Transfer (FRET) by exploiting FLIM at confocal speed.
The lifetime of autofluorescence and of fluorescent dyes changes depending on the local environmental conditions, such as pH, ion concentration, binding to other molecules, etc. The SP8 FALCON platform can measure these fast changes and provide metabolic/functional imaging. The additional lifetime contrast SP8 FALCON allows fluorophore separation.
Topics & Tags
Differentiation of different structures in histological stainings.
Lifetime Movie of Sepal of Silverberry
Observation of different microenvironments in Kidney slice with Lifetime Imaging.
Differentiation of different structures in multiphoton images.
Observing physiological processes at increased speed, see here the movement of chloroplasts in life Egeria plant.
The Lifetime measurement of FRET-sensors allows fast, easy and robust observation of the binding and unbinding of metabolic molecules, in this example: cAMP fluctuations.
FLIM-FRET: Single Molecule FRET
Measuring interaction at the single molecule level.
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