How to Successfully Implement the Leica Nano Workflow

 How_to_successfully_implement_the_Leica_Nano_workflow_teaser.jpg

Many electron microscope (EM) workflows start with sample fixation followed by sample preparation and EM imaging. However, samples displaying interesting behavior tend to be rare and finding the “right cell” can be time-consuming and tedious. The live-cell  CLEM workflow allows you to capture dynamic information related to a relevant biological process as it happens and put these observations into their ultrastructural context. The Leica Nano workflow streamlines this process to optimize your performance and increase your productivity. In this webinar, we will demonstrate the Leica Nano workflow using an example which shows how reproducible results can be obtained without compromising sample quality.

Speakers

Dr. Julia Koenig

Julia Koenig studied Biology at Dresden University of Technology. After finishing her PhD in 2015, she joined the Electron Microscopy Facility of Lucy Collinson at the Francis Crick Institute in London, working on a wide variety of projects as well as methods and workflow development. In January 2018, she joined Leica Microsystems as a product manager for EM sample preparation for cryo workflows.

Dr. Robert Kirmse

Robert Kirmse studied biology at the University of Heidelberg. After finishing his PhD at the German Cancer Research Center (DKFZ) in 2007 and staying at the DKFZ as a postdoctoral fellow he joined the group of Andreas Hoenger at the University of Colorado at Boulder in 2009. He then changed into industry in 2012 as a product manager and application expert for correlative microscopy at ZEISS. In 2019 he joined Leica to head the product management team for sample preparation. Robert has a broad experience in light and electron microscopy methods including correlative and cryo workflows.

In this webinar you will learn

  • How to maintain physiological conditions from cell culture until cryo fixation
  • How to optimize your live-cell imaging for live-cell CLEM applications
  • Easy transfer from the THUNDER Imager Nano to the EM ICE high pressure freezer
  • Retrieve your cell of interest in the resin block

Register to watch the webinar

*
*
*
*
*
*
*
*
By clicking on the SUBMIT button, I confirm that I have reviewed and agree with Leica Microsystems GmbH Terms of Use and their Privacy Policy. I also understand my privacy choices as they pertain to my personal data, as detailed in the aforementioned Privacy Policy under ‘’Your Privacy Choices’’.

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting?

  • Leica Microsystems Inc.
    1700 Leider Lane
    Buffalo Grove, IL 60089 United States
    Office Phone : +1 800 248 0123
    Service Phone : 1 800 248 0223
    Fax : +1 847-236-3009

You will find a more detailed list of local contacts here.