How to Uncover Hidden Dimensions in Research with Lifetime Imaging

Roquemore, Liz

April 15, 2020 Article

How to Uncover Hidden Dimensions in Research with Lifetime Imaging

Enhance your research with FLIM

The vast majority of imaging experiments measure fluorescence intensity but there is another key property of fluorescence—its lifetime, which is information that comes “for free” with every experiment.

In this article we explore what lifetime imaging is, and how it can improve quality and add depth to your applications.

Authors

Liz Roquemore , PhD

Leica Microsystems

Topics & Tags

Confocal Microscopy

Unlike fluorescence intensity, lifetime is concentration-independent, so it can help you overcome or avoid imaging artifacts.

At the same time, the lifetime of fluorescent probes can be sensitive to environmental conditions, enabling you to monitor dynamic changes in temperature, pH, ion concentration, polarity and many other parameters and analytes that would be difficult or impossible to follow by conventional techniques.

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How to Uncover Hidden Dimensions in Research with Lifetime Imaging

Enhance your research with FLIM

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