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IL-18 cytokine derived from the enteric nervous system is important for intestinal immunity

Interleukin 18 (IL-18) is a proinflammatory cytokine, which induces cell-mediated immune reaction upon bacterial infection. In the intestine, it is known that IL-18 is produced in immune and epithelial cells. The researchers, Jarrett et al., discovered that there is also IL-18 derived from the enteric nervous system. Moreover, it turns out that this enteric-nervous-system-derived IL-18 is required for intestinal immunity. First, the researchers could localize IL-18 in the enteric neurons, using immunofluorescence microscopy. Second, they showed that IL-18 is produced in enteric neurons, using single molecule fluorescence in situ RNA hybridization (smFISH). For this, they used the THUNDER Imager 3D Live Cell with Computational Clearing.

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Enteric Nervous System-Derived IL-18 Orchestrates Mucosal Barrier Immunity

A. Jarret, R. Jackson, C. Duizer, M.E. Healy, J. Zhao, J.M. Rone, P. Bielecki, E. Sefik, M. Roulis, T. Rice, K.N. Sivanathan, T. Zhou, A.G. Solis, H. Honcharova-Biletska, K. Vélez, S. Hartner, J. Siong Low, R. Qu, M.R. de Zoete, N.W. Palm, A.M. Ring, A. Weber, A.E. Moor, Y. Kluger, R. Nowarski, R.A. Flavell, CELL (2020) vol. 180, iss. 1, E12, pp. 50-63, DOI: 10.1016/j.cell.2019.12.016