How to Improve Live Cell Imaging with Leica Nano Workflow

HeLa Kyoto cells (HKF1, H2B-mCherry, alpha Tubulin, mEGFP). Left image: Maximum projection of a z-stack prior to ICC and LVCC. Right image: Maximum projection of a mosaic z-stack after ICC and LVCC. How_to_improve_live_cell_imaging_with_Leica_Nano_Workflow_teaser.jpg

For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on the advantages of working with a widefield system and which obstacles need to be overcome when using sapphire as a cell-culture substrate.

Key Advantages

  • Fast cellular events can be captured for subsequent examination by Cryo Electron Microscopy.
  • Benefit from the Leica innovative technology Computational Clearing.
  • Efficient removal of out-of-focus blur.
  • Accurate targeting of proteins or cellular structures of interest for downstream ultrastructural analysis.

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