Changes in kidney substructures reveal renal pathologies
The podocyte cell layer in the glomerular filtration barrier plays a central role in kidney disease. Alterations in the architecture of the podocytes are linked to the onset of renal failure. Therefore, the ability to image this layer at high resolution is key to identify healthy and unhealthy tissue.
The size of the podocyte layer requires imaging techniques with a resolution well below the diffraction limit. This has been typically addressed with electron microscopy techniques, demanding complex sample preparation protocols and where three dimensional information is missing.
3D STED Deep Nanoscopy opens a new door for kidney research. The combination of 3D STED Nanoscopy with the Leica TCS SP8 STED 3X and optical clearing enables imaging the kidney with unprecedented three-dimensional detail in light microscopy, and with protein specificity.
Visualize the ultrastructure of the glomerular filtration barrier by 3D STED Deep Nanoscopy
Even deeper than 150 µm within the tissue, the ultrastructural features of the specimen come to light powered by the new STED WHITE glycerol objective lens. This is achieved not only at 23°C, but also under live cell imaging conditions at 37°C with a comfortable working distance of 300 µm.
Forget the hassle of physical sectioning. Enjoy spectral freedom and keep up with the growing palette of fluorescent biomarkers.
Be at the forefront of clinical research.
3D STED Deep Nanoscopy Workflow
For Cleared Kidney Samples
Nanoscopy with the TCS SP8 STED system equipped with the HC PL