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Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and intracellular bacterial pathogens, such as Salmonella typhimurium and Mycobacterium marinum. Micro-injection of bacteria and multi-color fluorescence imaging are essential techniques involved in the application of zebrafish embryo infection models.

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Benard EL, van der Sar AM, Ellett F, Lieschke GJ, Spaink HP, and Meijer AH:

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

J. Vis. Exp. 61: e3781 (2012); doi: 10.3791/3781

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