IIT – Italian Institute of Technology, Nanophysics, Genoa, Italy
The Italian Institute of Technology (IIT) is a foundation established jointly by the Italian Ministry of Education, Universities and Research and the Ministry of Economy and Finance to promote excellence in basic and applied research and to contribute to the economic development of Italy. The primary goals of the IIT are the creation and dissemination of scientific knowledge as well as the strengthening of Italy's technological competitiveness. To achieve these two goals, the IIT will cooperate with both academic institutions and private organizations, fostering through these partnerships scientific development, technological advances and training in high technology.
http://www.iit.it/en/home.html
The Nanophysics unit, established at the Italian Institute of Technology and abbreviated to "NANOPHYS", aims to design, realize and utilize advanced methodologies and instrumentations within the framework of optical spectroscopy and microscopy, scanning force microscopy and optical nanoscopy.
The unit is oriented to the study and characterization of nanostructured, biological and hybrid materials at the nanoscale, i.e. having at least one of the here spatial dimensions controllable at the nanometric or subnanometric scale.
Time-resolved STED microscopy
Introduced more than 30 years ago, stimulated emission depletion (STED) microscopy has raised to a standard and widely used method for imaging in the life sciences. Thanks to continuous technological…Read articleSTED-FLCS: An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics
Heterogeneous diffusion dynamics of molecules play an important role in many cellular signaling events, such as of lipids in plasma membrane bioactivity. However, these dynamics can often only be…Read articleAbstracts of the 5th European Super-Resolution User-Club Meeting
The 5th Super-Resolution User Club Meeting was held in collaboration with Professor Kees Jalink and The Netherlands Cancer Institute (NKI) in Amsterdam. Having the meeting at a location where…Read articleSTED Nanoscopy: A Glimpse into the Future
The well-known saying of "Seeing is believing" became even more apt in biology when stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel laureate S. Hell and coworkers.…Read articleNanoscale Protein Diffusion by STED-Based Pair Correlation Analysis
We describe for the first time the combination between cross-pair correlation function analysis (pair correlation analysis or pCF) and stimulated emission depletion (STED) to obtain diffusion maps at…Read articleAbstracts of the 4th European Super-Resolution User-Club Meeting
The 4th Super-Resolution User Club Meeting was held in collaboration with Christian Eggeling and the Weatherall Institute of Molecular Medicine in Oxford, UK. Here we present the abstracts of the…Read articleSingle-Wavelength Two-Photon Excitation-stimulated Emission Depletion (SW2PE-STED) Superresolution Imaging
Two-photon microscopy, multiphoton microcopy and super-resolution imaging. We developed a new class of two-photon excitation–stimulated emission depletion (2PE-STED) optical microscope. In this…Read articleAbstracts of the 3rd European Super-Resolution User-Club Meeting
The 3rd meeting of the Leica Super-Resolution User Club was held from June 17th to 19th, 2013 in collaboration with Alberto Diaspro and the Italian Institute of Technology (IIT) in Genoa. Confocal and…Read articleSTED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects
In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This…Read articleAbstracts of the 2nd European Super-Resolution User-Club Meeting
The 2nd meeting of the Leica Super-resolution User club was held from September 25 to 27, 2012 in collaboration with the Science for Life Laboratory at the Karolinska Institute, Stockholm, Sweden. …Read articleDevelopments in Multiphoton Excitation Microscopy
Basics, history, and applications of multiphoton microscopy. Honouring Goeppert-Mayer’s prediction of simultaneous two-photon absorption by an atom or molecule reported in the 1930s in her PhD…Read articleOrder versus Disorder
In modern biomaterial design the generation of an environment mimicking some of the extracellular matrix features is envisaged to support molecular cross-talk between cells and scaffolds during tissue…Read articleStem Cell Biology in Cancer Research
The comprehension of stem cell biology and its molecular basis is now acquiring paramount importance in cancer research. The need to look at a single, possibly living, cell makes fluorescence…Read articleHigh Data Output and Automated 3D Correlative Light–Electron Microscopy Method
Correlative light/electron microscopy (CLEM) allows the simultaneous observation of a given subcellular structure by fluorescence light microscopy (FLM) and electron microscopy. The use of this…Read article