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IMBA – Institute of Molecular Biotechnology GmbH, Vienna, Austria

  • Immersion Freezing of Suspended Particles and Cells for Cryo-Electron Microscopy

    Immersion freezing of thin aqueous specimens is an essential preparation technique for cryo-transmission electron microscopy (cryo-TEM), aiming to preserve fragile biological structures such as molecules and cells in their hydrated environment for a close-to-native visualization. For successful experiments, vitreous ice must be produced, surface contamination must be avoided, and, most important, the natural state of the structure must be preserved.
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