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Institut Pasteur, Unit of Dynamic Neuronal Imaging, Department of Neuroscience, Paris, France

Institut Pasteur
Unit of Dynamic Neuronal Imaging
Department of Neuroscience
25 rue du Dr. Roux
75015 Paris

  • Gated STED Microscopy with CW-STED Lasers

    Among the major steps in the development of Stimulated Emission Depletion (STED) microscopy, the demonstration of the use continuous wave lasers (CW-STED) was certainly contributing the most to a wide dissemination of the method due to the affordability and elegant simplicity of this implementation. Nevertheless, CW-STED was so far not reaching the same spatial resolution as pulsed-lasers STED configurations. A recent investigation on the time-course of the fluorescence emission probability in CW-STED has revealed the benefit of using a gated fluorescence detection (gSTED) to improve further the resolution of C-S
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