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Leica Microsystems

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Leica Microsystems is a world leader in microscopes and scientific instruments. Founded as a family business in the nineteenth century, the company’s history was marked by unparalleled innovation on its way to becoming a global enterprise.

Its historically close cooperation with the scientific community is the key to Leica Microsystems’ tradition of innovation, which draws on users’ ideas and creates solutions tailored to their requirements. At the global level, Leica Microsystems is organized in three divisions, all of which are among the leaders in their respective fields: Life Science, Industry and Medical.

 

The company is represented in over 100 countries with 6 manufacturing facilities in 5 countries, sales and service organizations in 20 countries, and an international network of dealers. The company is headquartered in Wetzlar, Germany.

 

http://www.leica-microsystems.com/

  • Which Sensor is the Best for Confocal Imaging?

    The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.
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  • STED Nanoscopy at the forefront of cancer research

    Alison Dun is the postdoctoral facility manager for the Edinburgh Super-Resolution Imaging Consortium (ESRIC), Heriot-Watt University, Edinburgh, UK. She has used a large range of microscope techniques during her PhD work. In a video published by BBC News, Alison Dun explains how STED (stimulated emission depletion) microscopes help researchers in the fight against cancer by getting a better understanding of the processes that go wrong in the human body, thus giving hope for future treatments.
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  • Cleanliness Analysis with a 2-methods-in-1 solution

    This report explains how a 2-in-1 materials analysis solution, combining optical microscopy and chemical analysis by laser induced breakdown spectroscopy (LIBS), leads to an overall more efficient and cost-effective cleanliness workflow.
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  • Laser Microdissection Publication List

    This monthly updated reference list demonstrates the major application fields for laser microdissection in life science research.
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  • What Does 30,000:1 Magnification Really Mean?

    One important criterion concerning the performance of an optical microscope is magnification. This report will offer digital microscopy users helpful guidelines to determine the useful range of magnification values.
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  • Coherent Raman Scattering Microscopy Publication List

    CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their molecules. The two most important CRS techniques are Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS). The biochemical image contrast of CRS is in many ways complementary to the molecular contrast obtained in fluorescence microscopy. A second crucial advantage of these methods is that they preserve the specimen/sample in a near pristine state. This reference list presents current and basic papers on CRS microscopy.
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  • 3-Dimensional Imaging of Macroscopic Defects in Aluminum Alloys

    The investigation of macroscale defects in aluminum (Al) alloys with a rapid 3-dimensional (3D) imaging approach is described in this report. Aluminum (Al) alloys play an important role in the production of aircraft and vehicles, as well as products in other industries. Defects present in the Al alloy used for the production of aircraft, vehicles, or other products can have a significant effect on their quality, performance, and lifetime.
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  • See the Structure with Microscopy - Know the Composition with Laser Spectroscopy

    The advantages of a 2-in-1 materials analysis solution combining optical microscopy and laser induced breakdown spectroscopy (LIBS) for simultaneous visual and chemical inspection are described in this report. The basic principles of the 2-in-1 solution and a comparison between it and other common materials analysis methods, such scanning electron microscopy (SEM), are explained to demonstrate how a rapid, efficient workflow is achieved. A 2-in-1 analysis solution can reduce significantly the cost and time for obtaining material image and composition data. Such data are instrumental in assuring quality and reliability to make confident decisions quickly during production, quality control, failure analysis, and research and development in industries and fields, such as automotive and metallurgy.
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  • FLIM FRET and Biosensors: Versatile Tools for Biomedical Research

    Fluorescence Lifetime Imaging (FLIM) in combination with Förster Resonance Energy Transfer (FRET) has proven to be very beneficial for investigations in biomedical research for a wide range of structural elements and dynamic changes in cells. FRET allows to monitor molecular interactions, as the FRET signal depends strongly on the distance of the two FRET partners. This allows to investigate interaction of molecules, like ligand-receptor pairs, protein-protein interactions or interactions of effectors with DNA.
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  • Researchers Find a “Digital” Mechanism Behind Neuronal Changes from Learning

    Neurons react to learning and memory by activating synaptic connections. The mechanisms behind this fundamental process are complex and poorly understood. Researchers at Thomas Jefferson University have found that neuron plasticity operates in a “digital” fashion through nanomodules of discrete size that multiply and strengthen neuronal connections upon stimulation. This breakthrough was published on April 23rd in the journal Nature Neuroscience.
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  • Visualization of DNA Molecules

    Precise low angle rotary shadowing with heavy metals (like platinum) can be used in transmission electron microscopy (TEM) to observe molecular details of objects previously absorbed on a thin, low grain and electron-transparent carbon film. To achieve the highest contrast and better image quality, it is essential that the coating is directional, and it is given at a precise angle toward the sample. The fine grain of the metal layers and the homogeneous thickness of the coating material all over the sample surface are also crucial requirements to achieve high quality TEM images. This requires the method of e-beam evaporation a stream of evaporated material which is very directional, extremely homogeneous, cool and fine grained.
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  • Leica is MAD about LIBS

    When analyzing material for cleanliness testing, users normally just want a simple way to know whether the particle under scrutiny is normal debris or something more risky. Users can now “LIBS” it to acquire rapidly the composition and then move onto the next manufacturing step.
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  • Free Webinar-on-Demand: Basics of Microscope Optics

    Microscopy has become easier than ever before. Leica Microsystems offers state-of-the-art microscopes that allow to start microscopy work without a lot of training and expert skills.
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  • FCS - Fluorescence Correlation Spectroscopy

    FCS is a fluorescence-based measurement method. Fluorescent molecules passing through a strongly focused, fixed laser beam are excited for fluorescence emission. After passing a confocal pinhole, the emitted photons are registered using very sensitive detectors.
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  • FLIM FRET - Fluorescence Resonance Energy Transfer

    A typical application of FLIM is FLIM-FRET. FRET is a well-established technique to study molecular interactions. It scrutinizes protein binding and estimates intermolecular distances on an Angström scale as well. The SP8 FALCON system together with the integrated FRET analyzer provides FRET-efficiency and binding maps.
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  • Lifetime – a Proper Alternative

    „Way too complicated!“ - the notorious feedback when it comes to fluorescence lifetime measurements. This will change now! New technologies and new concepts for data evaluation, all implemented in the new Leica SP8 FALCON, render fluorescence lifetime imaging (FLIM) as fuss-free as ordinary confocal imaging. And by the way: with Leica FALCON you can record frames 10 times faster compared to the classical standard. And three (or more) dimensional image stacks or time series are generated in a snap. Four channels simultaneously? No problem! And of course there are tunable excitation wavelength both visible with white light lasers (WLL) and infrared (the latter for multiphoton microscopy). That should be reason enough to delve into fluorescence lifetime imaging. The picture shows a lifetime image of a mouse embryo. Recorded in 722 stitched tiles and fitted for four separate characteristic times. Recording time ca 1 hour – compared to ca 1 day with the classical approach.
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  • FLCS - Fluorescence Lifetime Correlation Spectroscopy

    Essentially, FCS can be performed with a continuous-wave laser. Using pulsed lasers allows even more sophisticated analysis possibilities, such as time-gated FCS or Fluorescence Lifetime Correlation Spectroscopy (FLCS). Both methods make use of the additional information obtained by the simultaneous measurement of the fluorescence lifetime.
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  • SP FLIM - Spectral Fluorescence Lifetime Imaging

    The SP8 FALCON is the ideal tool for spectral FLIM detection. No emission filters in front of the FLIM detectors are necessary. This grants a much higher flexibility to the experimental design.
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  • FLIM - Fluorescence Lifetime Imaging

    The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
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  • FCCS - Fluorescence Cross-Correlation Spectroscopy

    FCCS (Fluorescence Cross-Correlation Spectroscopy) can be measured using the Leica TCS SP8 FCS system. Similar to FCS , it analyzes fluorescence intensity fluctuations derived from a small observation volume.
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  • Rating the Quality of Steel

    This report describes optimal microscopy solutions for rating steel quality in terms of non-metallic inclusions and reviews the various international and regional standards concerning rigorous quality assessment methods, e.g., EN 10247, ASTM E45, DIN 50602, and ISO 4967.
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  • The Future of Fluorescence in Vascular Neurosurgery

    In vascular neurosurgery, surgical microscopes are used to provide a magnified and illuminated view of the surgical field. Although surgeons benefit greatly from the superb image quality and optical magnification offered by the microscope, current limitations of the microscope mean that surgeons have to rely on their color acuity to differentiate between different tissue types.
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  • Free Webinar on-Demand: AirTeach Software

    Wi-Fi Education solutions help teachers to make class more interactive and inspiring. With network capable microscope cameras and the Leica AirLab App learning content gets transferred directly to students’ viewing devices. This promotes teamwork and makes sharing easy in the classroom and beyond.
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  • Free Webinar-on-Demand: Mechanical pre-preparation and ion milling for SEM observation

    See how the unique combination of pre-preparation system and ion milling system makes fast site specific sample preparation for Scanning Electron Microscopy or optical microscopy possible.
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  • Free Webinar On-Demand: New Cleanliness Workflow from Leica and Pall

    Obtaining cleanliness results rapidly, accurately, and reliably is a significant advantage for manufacturers and component suppliers. For this reason, Pall Corporation and Leica Microsystems have joined efforts to offer a new, unique workflow solution customizable to your individual needs. Join our free webinar to get a sneak preview on Leica Microsystems' new fully implemented LIBS (Laser Induced Breakdown Spectroscopy) system. This new landmark technology enables you to easily conduct fast and reliable optical and chemical cleanliness analysis with only one system. See for yourself how Leica Microsystems' new software and system solution facilitates and accelerates the identification of nature and source of particle contamination on components.
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